Sharon, I do a CK34BE12 & P63 cocktail for basal cells & charge for only one stain. That being said, you can absolutely charge for 2 as P63 in nuclear & the CK34BE12 is cytoplasmic, so the pathologist can discern one from the other. I am considering setting it up as a double charge in the future if our volume for it continues to climb as it has been lately.
Glen Dawson BS, HT(ASCP) & QIHC IHC Manager Milwaukee, WI -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sebree Linda A Sent: Wednesday, March 30, 2011 11:10 AM To: Sharon.Davis-Devine; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Ventana's Basal Cell Cocktail Sharon, We're actually doing a triple stain of HMWCK + p63 + p504S but charging as a single stain. I am advocating charging for at least 2 of the 3 stains (done in 2 separate runs) since one can easily differentiate between 2 different chromogen colors. The jury's still out. Linda A. Sebree University of Wisconsin Hospital & Clinics IHC/ISH Laboratory DB1-223 VAH 600 Highland Ave. Madison, WI 53792 (608)265-6596 -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sharon.Davis-Devine Sent: Wednesday, March 30, 2011 9:42 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Ventana's Basal Cell Cocktail To all of you Histonetter's out there I need to know for those of you that are using the new Ventana antibody, CK34BE12 + p63, Basal Cell Cocktail, are you charging these as as one charge or two? Any information will be greatly appreciated. Thanks. Sharon Davis-Devine, CT (ASCP) Cytology-Histology Supervisor Carle Foundation Hospital Laboratory and Pathology Services 611 West Park Street Urbana, Illinois 61801 217-383-3572 sharon.davis-dev...@carle.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
