You should be able to get protocols directly from Ventana. I tried out their K and L ISH probes and they gave us the protocols. They also supplied us with the protocol we use for EBER ISH.
Linda A. Sebree University of Wisconsin Hospital & Clinics IHC/ISH Laboratory DB1-223 VAH 600 Highland Ave. Madison, WI 53792 (608)265-6596 -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Steven Wilkes Sent: Thursday, March 31, 2011 3:10 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Kappa/Lambda ISH on Ventana Hi Histonet! I have been tasked with validating Kappa and Lambda on our Ventana Benchmark XT machines. Does anyone who uses this platform have suggestions as to which Ventana protease works best (1, 2 or 3)? Additionally, any other information concerning the optimization (suggested incubation times, RNA regions etc.) would be greatly appreciated. Thanks in advance. Steven srwil...@gmail.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet