Are you talking about GMA or MMA plastic? I have not tried to do pentachrome on GMA but I have a great TC for plastics which I have used for years for bone and cartilage development. Nothing beats a pentachrome for arteries though so it might be worth pursuing, if I get a chance I will look over the protocol for pentachrome and offer some suggestions to you for GMA (that is what I use) sections. Contact me directly to remind me please.
Regards, Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech 12635 Montview Blvd. Ste.215 Aurora, CO 80045 720-859-4060 fax 720-859-4110 www.ihctech.net www.ihcrg.org -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Herrick, James L. (Jim) Sent: Friday, April 08, 2011 11:10 AM To: histonet Subject: [Histonet] Movat's Pentachrome Question Happy Friday everyone!! I am trying to find a good Modified Movat's pentachrome stain protocol for plastic embedded iliac arteries and also bone tissue (animal model). Everything I have been able to find so far, is for paraffin embedded tissue and a mile long. There may not be a fix for the length of the protocol but if anyone would happen to have a technique that works well with plastic, I would greatly appreciate it. Thank you much. Have a great weekend!! Jim _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet