Hi Folks.. Looking for a bit of help. Is there anyone out there who are doing their immuno's manually? Our Pathologist has just informed us that our immuno's are ALL too weak. He states that they always have been, as he compares them to slides that we get back from the reference labs that we use for the antibodies that we don't do in-house. If you are doing them manually...what companies are you using for antibodies, detection kit and chromogen? And are your stains really dark? I informed our Pathologist that I know for a fact that the reference labs that we use, do the immuno's on stainers, and naturally these stains will be consistently be stronger. I am trying to see if I can keep costs down by trying not to have to buy a stainer and continue to do the immuno's manually. If you all know of a wet-workshop or of a immuno. company who would send someone to our facility to teach us ( the latter is preferred, since my section chief is going to retire in 1 1/2 weeks, I will be taking over her duties and this is going to leave us with only myself and one other tech, until we can get a replacement for my position). Any help or advice that can be given will be greatly appreciated. Thanks so much. Valerie Hannen, MLT(ASCP),HTL,SU (FL) Parrish Medical Center Titusville,Florida
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