Hi, I am trying to get AKT working on formalin fixed paraffin embedded human surgical pathology tissue. I have tried two vendors (Cell Signalling and Epitomics) and I'm having terrible difficulty getting anything out of it. I was wondering if anyone else is using AKT (from anywhere) and is getting decent results. The PI here is getting really upset about it and I feel like I've done everything but stand on my head while doing the test.
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