We do a lot of IHC staining on bone and cartilage samples and we use 10% formic acid for decalcification for immunohistochemistry. I'm afraid you may not be able to turnaround this in a week. The tissue needs to be adequately fixed prior to decalcification, and properly decaled. I would trim the tissue to about 4 mm thick if you can on a saw you might be able to turn this around in your time frame, but I have learned from experience if you rush decalcification you have a greater chance of running into problems.
Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC PO Box 18592 Boulder, Colorado 80308 office (303) 682-3949 fax (303) 682-9060 www.premierlab.com Ship to Address: 1567 Skyway Drive, Unit E Longmont, Colorado 80504 -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Nejat Yilmaz Sent: Wednesday, April 20, 2011 8:12 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] decalcification of articular cartilage Dear Members, We're planning to study sheep knee articular cartilage with histochemistry and immunohistchemistry. Proximal parts of the tibia bones fixing in formaldehyde at the moment. We need an effective, quick and cartilage tissue protective decalcification method. We'll perform only light microscopic examination in this study and we have to complete the study within one week. In these circumstances what are your advices? Thanks in advance. Dr. Necat Yilmaz University of Mersin School of Medicine __________ ESET NOD32 Antivirus Akıllı Güvenlik tarafından sağlanan bilgiler, virüs imza veritabanı sürümü: 6057 (20110420) __________ İleti ESET NOD32 Antivirus Akıllı Güvenlik tarafından denetlendi. http://www.nod32.com.tr _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet