90 minutes for up to 2mm thickness using K3000 or VIP5. I do not have a VIP6
Steve A. McClain, MD McClain Laboratories, LLC 45 Manor Road Smithtown, NY 11787 631 361-4000 631 361-4037fax -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of histonet-requ...@lists.utsouthwestern.edu Sent: Thursday, April 21, 2011 12:51 PM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 89, Issue 24 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-requ...@lists.utsouthwestern.edu You can reach the person managing the list at histonet-ow...@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Cassette labeler (Martin, Gary) 2. 'Deplasticizing' before staining? (Mahesh Polavarapu) 3. Re: Request help on finding a part for Leitz 1512 (Henry Chidgey) 4. RELIA Histology Job Alert. Excellent opportunity on Long Island Day shift NY Lic required. (Pam Barker) 5. RE: Re: Request help on finding a part for Leitz 1512 (Weems, Joyce) 6. Re: Re: Request help on finding a part for Leitz 1512 (Grantham, Andrea L - (algranth)) 7. Part time Histo Tech position in Phoenix AZ (jco...@yahoo.com) 8. Unsubscribe (Tate Maria) 9. Re: Unsubscribe (Mollie Hannon) 10. Graduating in June from histo program (Teresa Moore) 11. RE: whole retinal staining (Abbey Mortimer) 12. How to Unsubscribe (Lee & Peggy Wenk) 13. trichrome/ frozen cardiac tissue (Molinari, Betsy) 14. UCP1 Antibody (John Shelley) 15. IHC validation (Troutman, Kenneth A) 16. cyto prep (Cynthia Pyse) 17. processing times for skin specimens (Carol Bryant) 18. IHC menu (Houston, Ronald) 19. Re: cyto prep (Sheila Haas) 20. Re: Unsubscribe (Emily Sours) 21. RE: cyto prep (Weems, Joyce) 22. Re: Unsubscribe (Merced Leiker) 23. eosin bleeding on frozen sections (Christi Cosby) 24. Neutralizing Formalin (Evans, Andria B) 25. RE: eosin bleeding on frozen sections (sgoe...@mirnarx.com) 26. (no subject) (SHANE NELSON) ---------------------------------------------------------------------- Message: 1 Date: Wed, 20 Apr 2011 10:12:34 -0700 From: "Martin, Gary" <gmar...@marshallmedical.org> Subject: [Histonet] Cassette labeler To: <histonet@lists.utsouthwestern.edu> Message-ID: <6ED9D4252F278841A0593D3D788AF24C0D480110@mailsvr.MARSHMED.local> Content-Type: text/plain; charset="us-ascii" We have just received a Lamb Micro Writer (model # E22.01MWI) single shoe. The problem is that we did not receive an operator manual. Does anyone know where I can get a manual? ------------------------------ Message: 2 Date: Wed, 20 Apr 2011 12:24:55 -0500 From: Mahesh Polavarapu <polavarapu.mah...@gmail.com> Subject: [Histonet] 'Deplasticizing' before staining? To: histonet <histonet@lists.utsouthwestern.edu> Message-ID: <BANLkTim6mC2iuyvCLeX5G=9fwspcjrq...@mail.gmail.com> Content-Type: text/plain; charset=ISO-8859-1 Hi, I have 50uM thick sections of soft tissue embedded in MMA (cold cured Technovit 9100). Do these need to be 'deplasticized' before they will take up stain? Thanks in advance! - Mahesh ------------------------------ Message: 3 Date: Wed, 20 Apr 2011 12:31:10 -0500 From: "Henry Chidgey" <hchid...@earthlink.net> Subject: [Histonet] Re: Request help on finding a part for Leitz 1512 To: <histonet@lists.utsouthwestern.edu> Message-ID: <016f01cbff80$bef19030$3cd4b090$@net> Content-Type: text/plain; charset="us-ascii" I was asked by a member to share the solution so here it is : Call Klaus Dern - 706-635-8840 - Microprecision Instruments - Ellijay, GA. He will probably have some or can make you some. Great guy and very knowledgeable. Thanks Joyce for giving me this informationJ Henry ------------------------------ Message: 4 Date: Wed, 20 Apr 2011 13:36:45 -0400 From: "Pam Barker" <rel...@earthlink.net> Subject: [Histonet] RELIA Histology Job Alert. Excellent opportunity on Long Island Day shift NY Lic required. To: "'Histonet'" <histonet@lists.utsouthwestern.edu> Message-ID: <DCFA9EE40E204519866E4B9E75992D67@ownerf1abaad51> Content-Type: text/plain; charset="iso-8859-1" Hi Histonetters!! I have a great new opportunity that I want to tell you about. I am working with a Laboratory located on Long Island that is in need of a NY licensed histology tech. This is a dayshift full time permanent position. My client offers a very competitive salary, excellent benefits, a great place to work and they are ready to interview and hire right away. If you or anyone you know might be interested in this position please contact me. I can be reached toll free at 866-607-3542 or rel...@earthlink.net. Thanks and have a great day!! Thank You! Pam Barker President RELIA Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 E-mail: rel...@earthlink.net www.facebook.com search Pam Barker RELIA www.linkedin.com/reliasolutions www.myspace.com/pamatrelia www.twitter.com/pamatrelia ------------------------------ Message: 5 Date: Wed, 20 Apr 2011 14:54:58 -0400 From: "Weems, Joyce" <jwe...@sjha.org> Subject: RE: [Histonet] Re: Request help on finding a part for Leitz 1512 To: "hchid...@earthlink.net" <hchid...@earthlink.net>, "histonet@lists.utsouthwestern.edu" <histonet@lists.utsouthwestern.edu> Message-ID: <92ad9b20a6c38c4587a9febe3a30e164081f4bb...@chexcms10.one.ads.che.org> Content-Type: text/plain; charset="us-ascii" He worked in the Leica factory and is truly an expert. -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Henry Chidgey Sent: Wednesday, April 20, 2011 13:31 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: Request help on finding a part for Leitz 1512 I was asked by a member to share the solution so here it is : Call Klaus Dern - 706-635-8840 - Microprecision Instruments - Ellijay, GA. He will probably have some or can make you some. Great guy and very knowledgeable. Thanks Joyce for giving me this informationJ Henry _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. ------------------------------ Message: 6 Date: Wed, 20 Apr 2011 12:51:48 -0700 From: "Grantham, Andrea L - (algranth)" <algra...@email.arizona.edu> Subject: Re: [Histonet] Re: Request help on finding a part for Leitz 1512 To: "hchid...@earthlink.net" <hchid...@earthlink.net> Cc: "histonet@lists.utsouthwestern.edu" <histonet@lists.utsouthwestern.edu> Message-ID: <daebdd23-2a85-4f2a-84d3-11a82925d...@email.arizona.edu> Content-Type: text/plain; charset="us-ascii" I second getting in touch with Klaus - he knows the 1512 inside and out. Andi Grantham On Apr 20, 2011, at 10:31 AM, Henry Chidgey wrote: > I was asked by a member to share the solution so here it is : > > Call Klaus Dern - 706-635-8840 - Microprecision Instruments - Ellijay, GA. > He will probably have some or can make you some. > > Great guy and very knowledgeable. Thanks Joyce for giving me this > informationJ > > Henry > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ------------------------------ Message: 7 Date: Wed, 20 Apr 2011 13:08:11 -0700 (PDT) From: jco...@yahoo.com Subject: [Histonet] Part time Histo Tech position in Phoenix AZ To: "Histonet@Lists. Utsouthwestern. Edu" <histonet@lists.utsouthwestern.edu> Message-ID: <58759.56388...@web161609.mail.bf1.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Part time Histo Tech position open with Southwest Skin Specialists, Ltd., located in Phoenix AZ. Responsibilities for this position include: grossing and fixation, processing, embedding, sectioning and staining. Education Requirements: Associate Degree in Applied Science/Histology and/or Histologic Certificate and HT or HTL ASCP Certification. If interested please send resume to miver...@swskin.net. Jill Cox, HT ASCP ------------------------------ Message: 8 Date: Wed, 20 Apr 2011 16:36:01 -0700 (PDT) From: Tate Maria <spanish_fly1...@yahoo.com> Subject: [Histonet] Unsubscribe To: histonet@lists.utsouthwestern.edu Message-ID: <383929.85727...@web125706.mail.ne1.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 please remove from this mailing list --- On Thu, 4/7/11, ti fei <ti...@foxmail.com> wrote: From: ti fei <ti...@foxmail.com> Subject: [Histonet] Unsubscribe To: "Vanessa Avalos" <vava...@allergydermatology.com>, "HISTONET LISTS" Date: Thursday, April 7, 2011, 9:26 AM Hi please remove me from the mailing list ------------------ Original ------------------ From: "Vanessa Avalos"<vava...@allergydermatology.com>; Date: Thu, Apr 7, 2011 05:54 AM To: "HISTONET LISTS"<histonet@lists.utsouthwestern.edu>; Subject: RE: [Histonet] Slides sent out for outside consult. Our practice does not bill the patient. Before sending they sign a waiver giving us permission to send out. On this waiver it states that if this is something their insurance does not cover then they may revieve a bill for $-$ range. We have an agreement with our consulting lab that they will only accept what the individual insurance covers. Our patients were getting ridiculous bills for consulting and our office was getting angry phone calls. After talking to the lab they made this agreement. -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Mike Pence Sent: Wednesday, April 06, 2011 2:36 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Slides sent out for outside consult. How is everyone handling a case that you send out to an outside consultant as far as billing the patient for the consult. Right now if I send out a case they bill me back an 88321. I am being told that I can not pass this same 88321 on to the patient. Having the outside consultant do the billing is not an option. Thanks, Mike _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -----Inline Attachment Follows----- _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 9 Date: Wed, 20 Apr 2011 20:21:18 -0400 From: Mollie Hannon <mol...@phylogenyinc.com> Subject: Re: [Histonet] Unsubscribe To: histonet@lists.utsouthwestern.edu Cc: histonet@lists.utsouthwestern.edu Message-ID: <81bcdb6f-2770-4c4b-833c-a55143e09...@phylogenyinc.com> Content-Type: text/plain; charset=us-ascii Please remove me from this list. Thank you. On Apr 20, 2011, at 7:36 PM, Tate Maria wrote: > > please remove from this mailing list > --- On Thu, 4/7/11, ti fei <ti...@foxmail.com> wrote: > > From: ti fei <ti...@foxmail.com> > Subject: [Histonet] Unsubscribe > To: "Vanessa Avalos" <vava...@allergydermatology.com>, "HISTONET LISTS" > Date: Thursday, April 7, 2011, 9:26 AM > > Hi please remove me from the mailing list > ------------------ Original ------------------ > From: "Vanessa Avalos"<vava...@allergydermatology.com>; > Date: Thu, Apr 7, 2011 05:54 AM > To: "HISTONET LISTS"<histonet@lists.utsouthwestern.edu>; > > Subject: RE: [Histonet] Slides sent out for outside consult. > > > Our practice does not bill the patient. Before sending they sign a waiver > giving us permission to send out. On this waiver it states that if this is > something their insurance does not cover then they may revieve a bill for > $-$ range. We have an agreement with our consulting lab that they will only > accept what the individual insurance covers. Our patients were getting > ridiculous bills for consulting and our office was getting angry phone > calls. After talking to the lab they made this agreement. > > -----Original Message----- > From: histonet-boun...@lists.utsouthwestern.edu > [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Mike Pence > Sent: Wednesday, April 06, 2011 2:36 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Slides sent out for outside consult. > > How is everyone handling a case that you send out to an outside > consultant as far as billing the patient for the consult. Right now if I > send out a case they bill me back an 88321. I am being told that I can > not pass this same 88321 on to the patient. Having the outside > consultant do the billing is not an option. > > Thanks, > Mike > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -----Inline Attachment Follows----- > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 10 Date: Wed, 20 Apr 2011 22:30:18 -0400 From: Teresa Moore <tmoor...@gmail.com> Subject: [Histonet] Graduating in June from histo program To: histonet@lists.utsouthwestern.edu Message-ID: <banlktim2wail9nkw2dqwfzd3vyr6lqe...@mail.gmail.com> Content-Type: text/plain; charset=ISO-8859-1 I will be graduating from the histology program at Columbus State Community College in June. Looking for a histotechnician position in or near Columbus, Ohio. Any advice or leads are appreciated. Thank you. Teresa Moore ------------------------------ Message: 11 Date: Thu, 21 Apr 2011 12:48:46 +1000 From: Abbey Mortimer <a.morti...@latrobe.edu.au> Subject: [Histonet] RE: whole retinal staining To: "David A. Wright" <d...@uchicago.edu>, "histonet@lists.utsouthwestern.edu" <histonet@lists.utsouthwestern.edu> Message-ID: <e44363415887e640b905f07380b6872f1a1fb9b...@exch2007.ltu.edu.au> Content-Type: text/plain; charset="iso-8859-1" Thank you all so very much! Abbey Mortimer PhD Candidate School of Psychological Science La Trobe University Bundoora, Victoria, 3083 AUSTRALIA Phone: (03) 9479 2470 Email: a.morti...@latrobe.edu.au ________________________________________ From: David A. Wright [d...@uchicago.edu] Sent: Wednesday, 20 April 2011 7:41 AM To: histonet@lists.utsouthwestern.edu Cc: Abbey Mortimer Subject: whole retinal staining Dear Abbey & Histonet I agree with René Buesa: I would try to stain whole retinae by a 'floating section' method i.e. hand processing in a dipping tray or mesh baskets (brush transfer can be done too but is laborious). Then mount them at the end. Floating allows reagents to penetrate from both sides of the retina - extra valuable because your incubation times are going to be very long with material this thick. (I assume you are not just interested in superficial layers.) A microwave processor would help with penetration, but I have no direct experience. -David == David A. Wright, Ph.D. University of Chicago Section of Neurosurgery ORIGINAL POST: Histonet Digest, Vol 89, Issue 21 Message: 7 Date: Tue, 19 Apr 2011 19:00:27 +1000 From: Abbey Mortimer <a.morti...@latrobe.edu.au> Subject: [Histonet] Tissue adhesion to slides Hello out there! I was wondering if any of you have experience in attaching human retinae (~250-300 microns thick) to slides for manual immunohistochemistry. Starfrost does not seem adequate. Any advice would be greatly appreciated! Regards, Abbey Abbey Mortimer PhD Candidate School of Psychological Science La Trobe University Bundoora, Victoria, 3083 AUSTRALIA Phone: (03) 9479 2470 Email: a.morti...@latrobe.edu.au ------------------------------ Message: 12 Date: Thu, 21 Apr 2011 05:48:41 -0400 From: "Lee & Peggy Wenk" <lpw...@sbcglobal.net> Subject: [Histonet] How to Unsubscribe To: "Histonet" <Histonet@lists.utsouthwestern.edu> Message-ID: <1EA881612BC34D52BB4B1C32ADA42CCE@HP2010> Content-Type: text/plain; charset="iso-8859-1" It's a do-it-yourself. - Scroll to bottom of any Histonet email. - Click on the link that has the word "lists" in it. - Scroll to the bottom of that page, where there is information on how to unsubscribe. - Follow directions. Peggy Wenki ------------------------------ Message: 13 Date: Thu, 21 Apr 2011 12:25:24 +0000 From: "Molinari, Betsy" <bmolin...@heart.thi.tmc.edu> Subject: [Histonet] trichrome/ frozen cardiac tissue To: "'histonet@lists.utsouthwestern.edu'" <histonet@lists.utsouthwestern.edu> Message-ID: <d0902470cecc5a45aec43366c474b62c349...@thiexch02.thi2.com> Content-Type: text/plain; charset="us-ascii" Hi, I have some frozen house hearts that were perfused with 4% paraformaldehyde. I found protocols for FF, but not fixed frozen. I tried staining them with my usual Gomori I use on my paraffin blocks but the doc thinks they picked up too much red, not enough green. Any suggerstions..I need to get them done today, they were brought up to RT this morning. Thanks. Betsy Molinari HT(ASCP) Texas Heart Institute Cardiovascular Pathology 6770 Bertner Ave MC1-283 Houston,TX 77030-2607 832-355-6524 832-355-6812 ------------------------------ Message: 14 Date: Thu, 21 Apr 2011 09:13:39 -0400 From: John Shelley <jshel...@sanfordburnham.org> Subject: [Histonet] UCP1 Antibody To: "histonet@lists.utsouthwestern.edu" <histonet@lists.utsouthwestern.edu> Message-ID: <a0e1854154c4bf4c9ef42a735fd19c741587e6f...@ln-mail07.ln.burnham.org> Content-Type: text/plain; charset="iso-8859-1" Hi Histonetters, I was hoping those of you who may have worked with UCP1 antibody might be willing to share how you optimized this antibody. I am using UCP1 antibody (ab10983) from Abcam. I have followed the datasheet and have used both protease and proteinase k and at varying times with no results. I am using brown fat from a wild type mouse and this is the control tissue that should work. I am running it overnight in a 4 ° to see if that might help since the Post Doc said that their western blot worked better that way. Any help or ideas how to proceed would be appreciated. Thanks in advance! Kind Regards! John J Shelley ------------------------------ Message: 15 Date: Thu, 21 Apr 2011 08:16:39 -0500 From: "Troutman, Kenneth A" <ashley.trout...@vanderbilt.edu> Subject: [Histonet] IHC validation To: "Histonet@lists.utsouthwestern.edu" <Histonet@lists.utsouthwestern.edu> Message-ID: <7b310892042da74cb3590053f424cfe613d63cd...@its-hcwnem06.ds.vanderbilt.edu> Content-Type: text/plain; charset="us-ascii" Hematoxylin should not affect the immunoreactivity (since counterstaining occurs following the IHC reaction), so no, changing the hematoxylin would not require a revalidation. Just make sure you are not obscuring the immunostain with an overly-dark counterstain. The point is to provide contrast between the DAB (brown) or fast red chromagen and to offer the reader a sense of context of the surrounding morphology of the tissue being stained. Ashley Troutman BS, HT(ASCP) QIHC Immunohistochemistry Supervisor Vanderbilt University Histopathology 1301 Medical Center Drive TVC 4531 Nashville, TN 37232 Message: 10 Date: Tue, 19 Apr 2011 14:04:32 -0500 From: "Ring, Mary L" <mary.l.r...@healthpartners.com> Subject: [Histonet] IHC validation To: "histonet@lists.utsouthwestern.edu" <histonet@lists.utsouthwestern.edu> Message-ID: <2594d39b69223047b6e2f4adf1e62db9010ffb487...@hpemx3.healthpartners.int> Content-Type: text/plain; charset="iso-8859-1" Does a change in the type of hematoxylin counterstain require any re-validation of IHC stains? Thanks for your help! Mary Ring, HT, QIHC Regions Hospital, St Paul, Mn ------------------------------ Message: 16 Date: Thu, 21 Apr 2011 09:51:35 -0400 From: "Cynthia Pyse" <cp...@x-celllab.com> Subject: [Histonet] cyto prep To: "'Histonet'" <histonet@lists.utsouthwestern.edu> Message-ID: <001501cc002b$3a929360$afb7ba20$@com> Content-Type: text/plain; charset="us-ascii" Good Morning Histonetters For those of you who have pap smears in their facility's, who do you have preparing the pap smears. Lab assistants, lab tech, or lab technologists? I am having a discussion with my general manager on who can prep pap smears. I decided to take a quick poll to see what everyone else is doing. The lab is in NYS so we fall under their guidelines, which I have checked. Just want as much info as possible from as many sources as I can. Thanks in advance for the responses. Cindy Pyse, CLT, HT (ASCP) Laboratory/Histology Supervisor X-Cell Laboratories 716-250-9235 ext. 232 e-mail cp...@x-celllab.com ------------------------------ Message: 17 Date: Thu, 21 Apr 2011 10:10:02 -0400 From: Carol Bryant <cb...@lexclin.com> Subject: [Histonet] processing times for skin specimens To: "'histonet@lists.utsouthwestern.edu'" <histonet@lists.utsouthwestern.edu> Message-ID: <50DA0C6B72976B4AB3A0FCA04CC73DBF05C77A9C7F@EXCHANGESB> Content-Type: text/plain; charset="us-ascii" Hello everyone! What is the shortest cycle you may be using for processing skin specimens? Can skin biopsies, shaves, and punches be processed well on a short run on the VIP 6? I need to find a successful protocol if possible to run skins on a 6 hour process, if possible. Thank you in advance for any input. Carol Carol Bryant, CT (ASCP) Cytology/Histology Manager Pathology Services Lexington Clinic Phone (859) 258-4082 Fax (859) 258-4081 cb...@lexclin.com NOTICE OF CONFIDENTIALITY This message, including any attachments, is intended only for the sole use of the addressee and may contain confidential or privileged information that is protected by the State of Kentucky and/or Federal regulations. If you are not the intended recipient, do not read, copy, retain or disseminate this message or any attachment. If you have received this message in error, please call the sender immediately at (859)258-4000 and delete all copies of this message and any attachment. Any unauthorized review, use, disclosure, copying or distribution is strictly prohibited. Neither the transmission of this message or any attachment, nor any error in transmission or misdelivery shall constitute waiver of any applicable legal privilege. ------------------------------ Message: 18 Date: Thu, 21 Apr 2011 10:00:22 -0400 From: "Houston, Ronald" <ronald.hous...@nationwidechildrens.org> Subject: [Histonet] IHC menu To: "histonet@lists.utsouthwestern.edu" <histonet@lists.utsouthwestern.edu> Message-ID: <e02e1309b208f94c83b968e45781001a2350036...@nchexmbx01.columbuschildrens.net> Content-Type: text/plain; charset="us-ascii" Would anyone be willing to share their IHC menu with us? I want to see how we compare with other top-class facilities. Obviously I am more interested in pediatric facilities, but we are constantly evaluating antibodies for research studies, and what may have applications in pediatric pathology from the adult world. I'd be happy to send our menu on return Thanks Ronnie Houston, MS HT(ASCP)QIHC Anatomic Pathology Manager ChildLab, a Division of Nationwide Children's Hospital www.childlab.com 700 Children's Drive Columbus, OH 43205 (P) 614-722-5450 (F) 614-722-2899 ronald.hous...@nationwidechildrens.org<mailto:ronald.hous...@nationwidechildrens.org> www.NationwideChildrens.org<http://www.NationwideChildrens.org> "One person with passion is better than forty people merely interested." ~ E.M. Forster ----------------------------------------- Confidentiality Notice: The following mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. The recipient is responsible to maintain the confidentiality of this information and to use the information only for authorized purposes. If you are not the intended recipient (or authorized to receive information for the intended recipient), you are hereby notified that any review, use, disclosure, distribution, copying, printing, or action taken in reliance on the contents of this e-mail is strictly prohibited. If you have received this communication in error, please notify us immediately by reply e-mail and destroy all copies of the original message. Thank you. ------------------------------ Message: 19 Date: Thu, 21 Apr 2011 07:23:36 -0700 (PDT) From: Sheila Haas <micropathl...@yahoo.com> Subject: Re: [Histonet] cyto prep To: Cynthia Pyse <cp...@x-celllab.com>, Histonet <histonet@lists.utsouthwestern.edu> Message-ID: <104166.87242...@web161712.mail.bf1.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 We have lab assistants running thin preps. Our cytotechs screen and do HPV. Sheila Haas Laboratory Supervisor MicroPath Laboratories, Inc. ________________________________ From: Cynthia Pyse <cp...@x-celllab.com> To: Histonet <histonet@lists.utsouthwestern.edu> Sent: Thu, April 21, 2011 9:51:35 AM Subject: [Histonet] cyto prep Good Morning Histonetters For those of you who have pap smears in their facility's, who do you have preparing the pap smears. Lab assistants, lab tech, or lab technologists? I am having a discussion with my general manager on who can prep pap smears. I decided to take a quick poll to see what everyone else is doing. The lab is in NYS so we fall under their guidelines, which I have checked. Just want as much info as possible from as many sources as I can. Thanks in advance for the responses. Cindy Pyse, CLT, HT (ASCP) Laboratory/Histology Supervisor X-Cell Laboratories 716-250-9235 ext. 232 e-mail cp...@x-celllab.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 20 Date: Thu, 21 Apr 2011 10:25:03 -0400 From: Emily Sours <talulahg...@gmail.com> Subject: Re: [Histonet] Unsubscribe To: Mollie Hannon <mol...@phylogenyinc.com>, spanish_fly1...@yahoo.com Cc: histonet@lists.utsouthwestern.edu Message-ID: <BANLkTim1Lp4=wws0eocoozzl5wsvpvm...@mail.gmail.com> Content-Type: text/plain; charset=UTF-8 Jupiter's thunder!!!! two people in a row? really?!!?!? LOOK AT THE BOTTOM OF THE EMAIL. YOU UNSUBSCRIBE ON THE SAME PAGE THAT YOU SUBSCRIBED ON. Both of you are officially banned from the internet. Forever. Don't use it again. And don't EVER join a mailing list. EVER. Emily, who would love to unsubscribe you so I didn't have to get your ridiculous emails about how you can't do it your damn self. A great book should leave you with many experiences, and slightly exhausted. You should live several lives while reading it. -William Styron ------------------------------ Message: 21 Date: Thu, 21 Apr 2011 10:31:13 -0400 From: "Weems, Joyce" <jwe...@sjha.org> Subject: RE: [Histonet] cyto prep To: Cynthia Pyse <cp...@x-celllab.com>, 'Histonet' <histonet@lists.utsouthwestern.edu> Message-ID: <92ad9b20a6c38c4587a9febe3a30e164081f4bb...@chexcms10.one.ads.che.org> Content-Type: text/plain; charset="us-ascii" We no longer do Gyn, but when we did we had lab assistants do the prep. Have lab asst for non-Gyn prep currently. J Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 678-843-7376 - Phone 678-843-7831 - Fax -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Cynthia Pyse Sent: Thursday, April 21, 2011 09:52 To: 'Histonet' Subject: [Histonet] cyto prep Good Morning Histonetters For those of you who have pap smears in their facility's, who do you have preparing the pap smears. Lab assistants, lab tech, or lab technologists? I am having a discussion with my general manager on who can prep pap smears. I decided to take a quick poll to see what everyone else is doing. The lab is in NYS so we fall under their guidelines, which I have checked. Just want as much info as possible from as many sources as I can. Thanks in advance for the responses. Cindy Pyse, CLT, HT (ASCP) Laboratory/Histology Supervisor X-Cell Laboratories 716-250-9235 ext. 232 e-mail cp...@x-celllab.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. ------------------------------ Message: 22 Date: Thu, 21 Apr 2011 10:34:44 -0400 From: Merced Leiker <lei...@buffalo.edu> Subject: Re: [Histonet] Unsubscribe To: Emily Sours <talulahg...@gmail.com>, Mollie Hannon <mol...@phylogenyinc.com>, spanish_fly1...@yahoo.com Cc: histonet@lists.utsouthwestern.edu Message-ID: <91f17a8ea5a1afda3fbca...@cdywxp1931.ad.med.buffalo.edu> Content-Type: text/plain; charset=us-ascii; format=flowed **Listserv master: Some people are reporting problems with managing their subscription as they have submitted their unsubscription multiple times in the prescribed manner and are still getting Histonet emails. This was reported to me by one such (failed) unsubscriber.** --On Thursday, April 21, 2011 10:25 AM -0400 Emily Sours <talulahg...@gmail.com> wrote: > Jupiter's thunder!!!! > two people in a row? really?!!?!? > LOOK AT THE BOTTOM OF THE EMAIL. YOU UNSUBSCRIBE ON THE SAME PAGE THAT > YOU SUBSCRIBED ON. > > Both of you are officially banned from the internet. Forever. Don't use > it again. > And don't EVER join a mailing list. EVER. > > Emily, who would love to unsubscribe you so I didn't have to get your > ridiculous emails about how you can't do it your damn self. > > A great book should leave you with many experiences, and slightly > exhausted. You should live several lives while reading it. > -William Styron > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > Merced M Leiker Research Technician III Cardiovascular Medicine 348 Biomedical Research Building State University of New York at Buffalo 3435 Main St, Buffalo, NY 14214 Ph: (716) 829-6118 Fx: (716) 829-2665 No trees were harmed in the sending of this email. However, many electrons were severely inconvenienced. ------------------------------ Message: 23 Date: Thu, 21 Apr 2011 11:18:23 -0500 From: Christi Cosby <christi.co...@gmail.com> Subject: [Histonet] eosin bleeding on frozen sections To: histonet@lists.utsouthwestern.edu Message-ID: <BANLkTi=1qmkuzfnq4s9ktpnndfroeyo...@mail.gmail.com> Content-Type: text/plain; charset=ISO-8859-1 Hi, In our lab we are having trouble with the eosin bleeding off the section after coverslipping. We are performing a rapid H&E stain on frozen sections. Any advice would be greatly appreciated! ------------------------------ Message: 24 Date: Thu, 21 Apr 2011 12:19:14 -0400 From: "Evans, Andria B" <aeva...@lghealth.org> Subject: [Histonet] Neutralizing Formalin To: "histonet@lists.utsouthwestern.edu" <histonet@lists.utsouthwestern.edu> Message-ID: <4182fdf23d7c9948bc41c4c082c3a54f021557b3a...@mail-ag-cluster.lha.org> Content-Type: text/plain; charset="iso-8859-1" Is anyone out there in Histoland Neutralizing their formalin before discarding it down the drain? We are looking into doing this because of our water authorities and we are finding that it is very costly. Has anyone found a cost efficient way to do this? I would need to know what supplier you are purchasing it from and the name of the product. Thank you in advance!! Andria B Evans HTL(ASCP)CM Lancaster General Hospital 555 North Duke Street Lancaster, PA 17604 (717)544-5511 ext: 77329 This email was sent securely from the LGHealth Email Service Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. ------------------------------ Message: 25 Date: Thu, 21 Apr 2011 11:26:56 -0500 From: <sgoe...@mirnarx.com> Subject: RE: [Histonet] eosin bleeding on frozen sections To: <christi.co...@gmail.com>, <histonet@lists.utsouthwestern.edu> Message-ID: <d957f2a7d21959488c492a2680f9920a223...@svrexch.asuragen.us> Content-Type: text/plain; charset="us-ascii" This happened to me a couple weeks ago. You got water on the slide after the eosin staining. Just run it back through alcohols, and rinse for about five minutes. Change out the alcohols, and re-stain. Sarah Goebel, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Christi Cosby Sent: Thursday, April 21, 2011 11:18 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] eosin bleeding on frozen sections Hi, In our lab we are having trouble with the eosin bleeding off the section after coverslipping. We are performing a rapid H&E stain on frozen sections. Any advice would be greatly appreciated! _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 26 Date: Thu, 21 Apr 2011 09:36:46 -0700 (PDT) From: SHANE NELSON <nelsonr...@verizon.net> Subject: [Histonet] (no subject) To: Histonet@lists.utsouthwestern.edu Message-ID: <193223.55791...@web84301.mail.re1.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Hi from Southern Cali, We neutralize our formalin with a product called Neutralex. It comes in powder form and after 30 mins we can dump it down the drain. We also keep records for QC purposes. Product is purchased from a company called American MasterTech. Hope this gives you a starting place. THANK YOU, PATTI RUBEN-NELSON H.T.(ASCP) PNP LABORATORY CONSULTANT SUPERVISOR/DESERT GASTROENTEROLOGY CONSULTANTS P.O. BOX 412 CABAZON, CA. 92230 cell (909) 841-9761 nelsonr...@verizon.net ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 89, Issue 24 **************************************** _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
