This is most likely caused by inadequate fixation in formalin which would protect the tissues from tissue processing. With such fast turn around times we are seeing this more and more. Put formalin on your tissue processor and use any extra time you may have up front in formalin rather than tissue processing. Ideally it takes 24 hrs to properly fix tissues so they are not damaged by processing and embedding in paraffin, but nobody fixes that long anymore.
Regards, Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech, LLC Fitzsimmons BioScience Park 12635 Montview Blvd. Suite 215 Aurora, CO 80010 P-720-859-4060 F-720-859-4110 wk email pru...@ihctech.net web site www.ihctech.net This email is confidential and intended solely for the use of the Person(s) ('the intended recipient') to whom it was addressed. Any views or opinions presented are solely those of the author. It may contain information that is privileged & confidential within the meaning of applicable law. Accordingly any dissemination, distribution, copying, or other use of this message, or any of its contents, by any person other than the intended recipient may constitute a breach of civil or criminal law and is strictly prohibited. If you are NOT the intended recipient please contact the sender and dispose of this e-mail as soon as possible. -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Thursday, May 12, 2011 1:41 PM To: Johnson, Nacaela; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] nuclear bubbling Nacaela: Heat by itself is not the cause, but the heat applied to sections that have not been properly drained and still have water left between them and the slide, is. You have to drain the sections properly before heating them. Using formalin is not the cause. René J. From: "Johnson, Nacaela" <nacaela.john...@usoncology.com> To: histonet@lists.utsouthwestern.edu Sent: Thursday, May 12, 2011 12:51 PM Subject: [Histonet] nuclear bubbling Has anyone one had problems with nuclear bubbling? I have read about two different causes. (1) Formalin itself is known to cause the issue and (2) heating the tissue at a high temp (70 degrees C or above) while drying. The latter is definitely not happening, but I do use formalin. I am in the process of changing the type of formalin that is used during collection. Does anyone have any other suggestions? Has this problem occurred for any other reason than I have already pointed out? It seems to be worse in the surgical needle biopsies than in the bone marrow biopsies. Thanks, Nacaela Johnson, B.S. HTL (ASCP)CM Histotechnologist KCCC Pathology 12000 110th St., Ste. 400 Overland Park, KS 66210 Office: 913-234-0576 Fax: 913-433-7639 Email: nacaela.john...@usoncology.com </pre>The contents of this electronic mail message and any attachments are confidential, possibly privileged and intended for the addressee(s) only.<br>Only the addressee(s) may read, disseminate, retain or otherwise use this message. If received in error, please immediately inform the sender and then delete this message without disclosing its contents to anyone.</pre> _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet