Here we just process starting in 70% EtOH, cut the section on a bath of 70% EtOH (watch out, it wrinkles very bad) and stain them in an Eosin only stain (which is the H&E with all of the aqueous parts taken out). The pathologist looks at them with a polarizing filter. Make sure that they stay away from water! A good article that talks about this is: Evaluation of Crystals in Formalin-Fixed, Paraffin-Embedded Tissue Sections for the Differential Diagnosis of Pseudogout, Gout, and Tumoral Calcinosis Shidham et. al. Mod Pathol 2001;14(8):806–810 Good luck!
On Mon, May 16, 2011 at 5:46 AM, Diana McCaig <dmcc...@ckha.on.ca> wrote: > Does anyone have a method for identifying uric acid crystals in gout on > a tissue sample. > > > > Diana > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Patrick Laurie HT(ASCP)QIHC CellNetix Pathology & Laboratories 1124 Columbia Street, Suite 200 Seattle, WA 98104 plau...@cellnetix.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet