We take frozen section from formalin fixed tissue and placed them in 30% sucrose overnight before mounting on chucks.
But never tried the other way round frozen tissue then fixed formalin, i heard that it helps in falling of the tissue. But no experience. Amita From: "Sherwood, Margaret " <msherw...@partners.org> To: <histonet@lists.utsouthwestern.edu> Date: 19/05/11 01:21 AM Subject: Re: [Histonet] Formalin fixed frozen sections Sent by: histonet-boun...@lists.utsouthwestern.edu Does anyone fix their frozen tissue first in formalin before embedding and sectioning? I know one investigator in our lab did that previously, but was not sure of the strength of formalin (I don't believe it was 10%). Another investigator inquired about doing that. I would appreciate hearing from fellow histonetters. Thanks! Peggy Peggy Sherwood Lab Associate, Photopathology Wellman Center for Photomedicine (EDR 214) Massachusetts General Hospital 55 Fruit Street Boston, MA 02114-2696 617-724-4839 (voice mail) 617-726-6983 (lab) 617-726-1206 (fax) msherw...@partners.org The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
