Dear Miss Margaret Blount: Congratulations on your retirement!! May you enjoy good health and many years doing the things you WANT to do!! God Bless!
-----Original Message----- From: histonet-request <histonet-requ...@lists.utsouthwestern.edu> To: histonet <histonet@lists.utsouthwestern.edu> Sent: Mon, May 23, 2011 1:00 pm Subject: Histonet Digest, Vol 90, Issue 28 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet r, via email, send a message with subject or body 'help' to histonet-requ...@lists.utsouthwestern.edu You can reach the person managing the list at histonet-ow...@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific han "Re: Contents of Histonet digest..." oday's Topics: 1. Immuno controls (Carrie Disbrow) 2. This is goodbye (Margaret Blount) 3. In vitro mouse embryo culture (Carmen Maria Garcia Pascual) 4. Re: Immuno controls (Rene J Buesa) 5. RE: Histonet Digest, Vol 90, Issue 27 (Barone, Carol ) 6. RE: Histonet Digest, Vol 90, Issue 27 (Barone, Carol ) 7. Freezing bath solutions (Scott, Allison D) 8. p63 (Joanne Clark) --------------------------------------------------------------------- Message: 1 ate: Sun, 22 May 2011 22:00:05 -0400 rom: Carrie Disbrow <cdisb...@msn.com> ubject: [Histonet] Immuno controls o: <histonet@lists.utsouthwestern.edu> essage-ID: <snt117-w41c388eec39a185424d7e1d0...@phx.gbl> ontent-Type: text/plain; charset="iso-8859-1" i all, fter consideration I disagree with the consensus and also agree with Curt's athologist. More supplies and labor will be involved as cutting more controls ill be neccessay to meet the requests of the pathologist. The patient tissue is ut on two controls slides. One slide is used as the negative control and has oth the patient tissue and the known positive control which should both be egative after the run. The other slide with the known positive control and the atient tissue in question is also stained with the positive control staining ositive. hank you! ------------------------------ Message: 2 ate: Mon, 23 May 2011 09:04:54 +0100 rom: "Margaret Blount" <ma...@medschl.cam.ac.uk> ubject: [Histonet] This is goodbye o: <histonet@lists.utsouthwestern.edu> essage-ID: <e2d19a5c4ef4c14fb2b012db6eebc36304778...@me-mail1.medlan.cam.ac.uk> ontent-Type: text/plain; charset="US-ASCII" To everyone in histoland, This is my last full week at work. From next Tuesday I will be retiring rom my job at Cambridge University. I have thoroughly enjoyed my chosen rofession as a research technician specialising in histology. I realise hat because I have always worked in research my experience is somewhat imited: I haven't been asked for the same range of techniques that you ind in a routine clinical lab. However, I have enjoyed my work and have earnt and devised some techniques for a range of interesting samples. ow it's time to learn something new! I hope to spend more time with my lace pillow and learn to do it roperly, maybe learn to do Northamptonshire lace which I believe is a ariation on Bucks point. (I come originally from Northants) I have had go at Bedfordshire and will learn more, it is very pretty. I also plan o travel; my next trip is to New Zealand in November. I won't go on. I just want to say this: You are all great guys and irls; you share your experience and friendship with great generosity, hich I have always felt is what a scientist should do. Thank you for our comradeship and help over the years. Maybe I'll get to meet some of ou by chance during my travels. Best wishes to you all and a great big Thank You, From Margaret Miss Margaret Blount Histology Manager Metabolic Research Laboratories Level 4 Institute of Metabolic Science Box 289, Addenbrooke's Hospital Hills Road, Cambridge, CB2 0QQ Tel 01223 769061/336079 ------------------------------ Message: 3 ate: Mon, 23 May 2011 10:35:20 +0200 (CEST) rom: "Carmen Maria Garcia Pascual"<carmen.m.gar...@uv.es> ubject: [Histonet] In vitro mouse embryo culture o: histonet@lists.utsouthwestern.edu essage-ID: <6395165382carma...@uv.es> ontent-Type: text/plain; charset="ISO-8859-1" Good Moorning to everyone: I am Carmen Garcia a PhD student from Valencia, we are know interesting n make in vitro culture of mouse embryos to do toxicity tests and in ur lab we have not experience. someone can give me some advise? or ecommend me some bibliography? ow long can I have the embryos in culture? hank you so much!!! Best regards, armen ------------------------------ Message: 4 ate: Mon, 23 May 2011 07:48:50 -0700 (PDT) rom: Rene J Buesa <rjbu...@yahoo.com> ubject: Re: [Histonet] Immuno controls o: Carrie Disbrow <cdisb...@msn.com>, "histonet@lists.utsouthwestern.edu" <histonet@lists.utsouthwestern.edu> essage-ID: <87688.31423...@web65714.mail.ac4.yahoo.com> ontent-Type: text/plain; charset=iso-8859-1 Also Sprach Zarathustra!!!! en� J. From: Carrie Disbrow <cdisb...@msn.com> o: histonet@lists.utsouthwestern.edu ent: Sunday, May 22, 2011 10:00 PM ubject: [Histonet] Immuno controls i all, fter consideration I disagree with the consensus and also agree with Curt's athologist. More supplies and labor will be� involved as� cutting more controls ill be neccessay to meet the requests of the pathologist. The patient tissue is ut on two controls slides. One slide is used as the negative control and has oth the patient tissue and the known positive control which should both be egative after the run. The other slide with the known positive control and the atient tissue in question is also stained with the positive control staining ositive. hank you! ��� ��� ��� � ��� ��� � _______________________________________________ istonet mailing list isto...@lists.utsouthwestern.edu ttp://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 5 ate: Mon, 23 May 2011 11:02:16 -0400 rom: "Barone, Carol " <cbar...@nemours.org> ubject: [Histonet] RE: Histonet Digest, Vol 90, Issue 27 o: <histonet@lists.utsouthwestern.edu> essage-ID: <da71f2870e979549818d426efd7a37ad855...@wlmmsx02.nemours.org> ontent-Type: text/plain; charset="us-ascii" -----Original Message----- rom: histonet-boun...@lists.utsouthwestern.edu mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of istonet-requ...@lists.utsouthwestern.edu ent: Sunday, May 22, 2011 1:01 PM o: histonet@lists.utsouthwestern.edu ubject: Histonet Digest, Vol 90, Issue 27 Send Histonet mailing list submissions to To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet r, via email, send a message with subject or body 'help' to histonet-requ...@lists.utsouthwestern.edu You can reach the person managing the list at histonet-ow...@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than Re: Contents of Histonet digest..." oday's Topics: 1. to Rene J Buesa (Maxim Peshkov) --------------------------------------------------------------------- Message: 1 ate: Sun, 22 May 2011 13:43:07 +0400 rom: Maxim Peshkov <maxim...@mail.ru> ubject: [Histonet] to Rene J Buesa o: Rene J Buesa <rjbu...@yahoo.com> c: histonet@lists.utsouthwestern.edu essage-ID: <1585823684.20110522134...@mail.ru> ontent-Type: text/plain; charset=windows-1251 I am sorry, that uses histonet for personal goals. Dear Rene! ast 3 my answers for you are returned back to me. our e-mails I got successfully. upport service of mail.ru wrote that problem is at yahoo.com server. lease check your postmaster service. Here is my post-address: axim Peshkov, l. Mikhailovskaya, 90-A, 47909, aganrog, ussia. Sincerely, axim. mailto:maxim...@mail.ru ----------------------------- _______________________________________________ istonet mailing list isto...@lists.utsouthwestern.edu ttp://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 90, Issue 27 *************************************** ------------------------------ Message: 6 ate: Mon, 23 May 2011 11:15:34 -0400 rom: "Barone, Carol " <cbar...@nemours.org> ubject: [Histonet] RE: Histonet Digest, Vol 90, Issue 27 o: <histonet@lists.utsouthwestern.edu> essage-ID: <da71f2870e979549818d426efd7a37ad855...@wlmmsx02.nemours.org> ontent-Type: text/plain; charset="us-ascii" Histonetters: Needed to stain xenograft with "Bunny"-IgG-direct, that ad already been in formailin two weeks (was FFPE). Normally would do n a frozen 1:40? ....We have background everywhwere on first un...suggestions? Neg is also shining??? Crazy researcher's! Got to ove them...when will they learn to ask first? We are diluting primary in plan two, and will stain over-night at 4 egrees....but, this thing is lit-up like the SUN! was expecting low signal? Go figure? ----Original Message----- rom: histonet-boun...@lists.utsouthwestern.edu mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of istonet-requ...@lists.utsouthwestern.edu ent: Sunday, May 22, 2011 1:01 PM o: histonet@lists.utsouthwestern.edu ubject: Histonet Digest, Vol 90, Issue 27 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet r, via email, send a message with subject or body 'help' to histonet-requ...@lists.utsouthwestern.edu You can reach the person managing the list at histonet-ow...@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than Re: Contents of Histonet digest..." oday's Topics: 1. to Rene J Buesa (Maxim Peshkov) --------------------------------------------------------------------- Message: 1 ate: Sun, 22 May 2011 13:43:07 +0400 rom: Maxim Peshkov <maxim...@mail.ru> ubject: [Histonet] to Rene J Buesa o: Rene J Buesa <rjbu...@yahoo.com> c: histonet@lists.utsouthwestern.edu essage-ID: <1585823684.20110522134...@mail.ru> ontent-Type: text/plain; charset=windows-1251 I am sorry, that uses histonet for personal goals. Dear Rene! ast 3 my answers for you are returned back to me. our e-mails I got successfully. upport service of mail.ru wrote that problem is at yahoo.com server. lease check your postmaster service. Here is my post-address: axim Peshkov, l. Mikhailovskaya, 90-A, 47909, aganrog, ussia. Sincerely, axim. mailto:maxim...@mail.ru ----------------------------- _______________________________________________ istonet mailing list isto...@lists.utsouthwestern.edu ttp://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 90, Issue 27 *************************************** ------------------------------ Message: 7 ate: Mon, 23 May 2011 11:22:24 -0500 rom: "Scott, Allison D" <allison_sc...@hchd.tmc.edu> ubject: [Histonet] Freezing bath solutions o: <histonet@lists.utsouthwestern.edu> essage-ID: <1872b4a455b7974391609ad8034c79fc026df...@lbexch01.hchd.local> ontent-Type: text/plain; charset="us-ascii" Hello to all in histoland. What type of solutions are being used if any o freezze your chucks for frozen sections. Our sister hospital is sing methyl butane and there was a flash fire last week. We use the ame solution. Any suggestions will be greatly appreciated. Allison Scott HT(ASCP) istology Supervisor BJ Hospital ONFIDENTIALITY NOTICE: f you have received this e-mail in error, please immediately notify the ender by return e-mail and delete this e-mail and any attachments from our computer system. To the extent the information in this e-mail and any attachments contain rotected health information as defined by the Health Insurance Portability nd Accountability Act of 1996 ("HIPAA"), PL 104-191; 45 CFR Parts 160 and 64; or Chapter 181, Texas Health and Safety Code, it is confidential and/or rivileged. This e-mail may also be confidential and/or privileged under exas law. The e-mail is for the use of only the individual or entity named bove. If you are not the intended recipient, or any authorized epresentative of the intended recipient, you are hereby notified that any eview, dissemination or copying of this e-mail and its attachments is trictly prohibited. ------------------------------ Message: 8 ate: Mon, 23 May 2011 16:31:08 +0000 rom: Joanne Clark <jcl...@pcnm.com> ubject: [Histonet] p63 o: "histonet@lists.utsouthwestern.edu" <histonet@lists.utsouthwestern.edu> essage-ID: <0494a7d4e8cc254ea2fb81464982e3786dc...@s10maild001n1.sh10.lan> ontent-Type: text/plain; charset="us-ascii" I need to find a source for p63 antibody. Where does everyone purchase theirs rom? Joanne Clark,HT athology Consultants of New Mexico ------------------------------ _______________________________________________ istonet mailing list isto...@lists.utsouthwestern.edu ttp://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 90, Issue 28 * _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet