Hi Christopher, There are differences between how Xylene substitutes works and Xylene. First of all, Xylene subs can be a bit slower to deparaffinize, so you may need to increase the time or # or stations when deparaffinizing. Also, Xylene subs don't tolerate water so your last alcohol must be 100% or else you will see the eosin bleed out of the sections. The other thing about Xylene substitutes is that the mounting media must be compatible with the substitute. If I can help in any other way, please let me know. thanks
Debbie Siena HT(ASCP)QIHC Technical Manager | StatLab Medical Products Direct: 972-436-1010 x229 -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Christopher Conlisk Sent: Friday, June 03, 2011 1:18 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Slide Bright Question Hello Everyone, I have worked in labs that use Xylene my entire career and I just started at a Lab That only uses a Xylene Substitute "Slide-Bright". I am having problems with the H&E. After staining and coverslipping (The slides look fine innitially), Then about 5-10 minutes after coverslipping the Eosin starts bleeding out all around the tissue. I have asked several of my Histotech Friends that are old timers and they say that Xylene Substitutes are awful at deparrifinization and awful at clearing. They told me that the alcohol isnt getting thoroughly cleared in the "Slide Brite" and then it is eventually leeching out after coverslipping??? Is this true and does anyone have any guidance for this issue? We also run MOHS slides on the same stainer and I keep all the reagents clean as a whistle. I really hate Xylene Substitute's.... Thanks C.S. Conlisk HT(ASCP), PBT(ASCP) Kansas City Skin and Cancer Center _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet