I failed to mention that I make up my Karnovsky's and freeze aliquots. I have never had a problem.
-----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Suhyoung Jeong Sent: Monday, June 06, 2011 8:39 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Pre-made paraformaldehyde question Dear Histo people, In my old lab, I have been taught that 4% PFA has to be always made fresh (maybe a day before max). Here in my new lab, I find that people use 32% pre-made PFA from EMS (without expiry date) and only dilute it on the day of use. Does anyone have experience (or thoughts) with this issue? Thank you in advance All the best, Suh _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet