You had COTTON in your pipettes? We used hollowed out reeds with a bit of papyrus in one end. No calibration.... But plenty accurate enough for histo in those days.
However, there are a few things I do not miss from back in da' day. Hand-stropping a knife for an hour, only to have it nicked by a staple in the next hour. -Bill -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of histot...@imagesbyhopper.com Sent: Sunday, June 19, 2011 7:48 PM To: Paula Sicurello Cc: histonet@lists.utsouthwestern.edu; Amos Brooks Subject: Re: [Histonet] Retirement And remember mouth pipeting? "oops, that's the cotton..." Eating and smoking in the lab was the norm. Our alcohol had the tax stamp on it! :o) Michelle On Jun 19, 2011, at 4:19 PM, Paula Sicurello <pat...@gmail.com> wrote: > Sheesh! We used to have people smoke while working with propylene oxide. > > Eating in your control pigs was part of the benefit of being a > graduate student to save on grocery money. > > Film? My TEM used glass plates. > > Lab mates used to routinely drink diet coke and 100% ethanol on Fridays. > > Wearing closed toed shoes was for wimps, you were just fast if you > dropped a steel wedge blade. > > We even wrote using the entire word and proper grammar, none of this > acronym stuff for us. > > Retirement? What's that? > > Paula :-) > > On Sun, Jun 19, 2011 at 12:31 PM, Amos Brooks <amosbro...@gmail.com> wrote: >> Agarose Gels! >> ... Listen you whipersnapper Agarose is the easy way out. When I >> learned it we used to have to make up our own polyacrylamide gels. >> That was after having to walk to work up hill both ways in 30 feet of snow! >> >> (No nearer retirement) >> Crotchety Amos >> >> >> >> Message: 7 >> Date: Fri, 17 Jun 2011 13:24:12 -0400 >> From: Emily Sours <talulahg...@gmail.com> >> Subject: Re: [Histonet] Retirement >> To: histonet@lists.utsouthwestern.edu >> Message-ID: <banlktinejxtxyop-byfweuxn3yw-ff3...@mail.gmail.com> >> Content-Type: text/plain; charset=UTF-8 >> >> Retirement? I think by the time I get to that point, social security >> will have run out. >> Then again, technology will be so advanced, I can tell stories about >> the old days, where I logged on to the bbs by modem to post messages >> to my friends and typed in my own html coding. >> We didn't have google when I was young!! Our cameras used film! And >> you couldn't see how bad your pictures were until you developed that film!! >> There was no PCR to sequence your DNA, you ran an agarose gel and >> hoped for the best!! You could drink the 100% ethanol, there was no >> denaturing! (okay that was before my time) You could smoke in the lab >> while you sectioned without gloves!! (okay that was too) >> >> Emily >> >> A great book should leave you with many experiences, and slightly exhausted. >> You should live several lives while reading it. >> -William Styron >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet