Hello Histonet, Does anyone have a working protocol that stains directly for mouse IgG (fluorescent) on frozen sections? We are currently staining with a goat anti mouse IgG 555 (highly cross absorbed) and we have having a hard time deciding what is specific staining and what is no. Information such as micron thickness, method of fixation (acetone/methanol, NBF etc) and staining parameters would be great!
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