We do put controls on each slide in a case. Sometimes it's just one slide that failed in a run. A batch control wouldn't tell you which slide failed if there are no internal controls in the patient tissue. I personally wouldn't feel comfortable doing IHC with batch controls. Mark
On Wed, Jul 20, 2011 at 6:18 AM, Rathborne, Toni < trathbo...@somerset-healthcare.com> wrote: > All of these responses are great. So here's a follow up question. > Do you place a control tissue on EACH slide if you have multiple blocks for > a case, or just on one of the slides? > > -----Original Message----- > From: Richard Cartun [mailto:rcar...@harthosp.org] > Sent: Tuesday, July 19, 2011 6:40 PM > To: histonet@lists.utsouthwestern.edu; Rathborne, Toni > Subject: Re: [Histonet] Controls with patient specimen on same slide > > We do not put our positive control tissue on the test slide; we run batch > controls. Many of the unstained slides (breast, GI, and prostate biopsies) > that we use for IHC testing are cut in our Histology Laboratory as part of a > part-type slide protocol. For example, we cut 7 slides, 2 sections on each > slide, for breast biopsies and stain #1, 4, and 7 with H&E, and then use (if > needed) #2, 3, 5, and 6 for IHC. Therefore, it would be very difficult for > us to place the positive control tissue on the same slide. In addition, I > receive a lot of consult cases from other hospitals where they send us > unstained slides for testing. Once again, it would be difficult to place > the positive control tissue on the same slide and it would slow us down in > terms of starting those slides once they arrive. However, I think the main > reason we don't pursue putting the positive control tissue on the same slide > is the fact that it would consume an enormous amount of control tissue. > > Richard > > Richard W. Cartun, MS, PhD > Director, Histology & Immunopathology > Director, Biospecimen Collection Programs Assistant Director, Anatomic > Pathology Hartford Hospital > 80 Seymour Street > Hartford, CT 06102 > (860) 545-1596 Office > (860) 545-2204 Fax > > > >>> "Rathborne, Toni" <trathbo...@somerset-healthcare.com> 7/19/2011 > >>> 3:27 PM >>> > > Hi, > I'm interested in knowing how many of you are performing ihc with the > control tissue and the patient tissue on the same slide. I have seen slides > available which have designated areas for each tissue to be placed so there > will not be any confusion. If you're doing it, have you encountered any > problems? What benefits have you noticed since implementing this process? > Are your pathologists in favor of this? If you're not, why not? > Thanks, > Toni > > > CONFIDENTIALITY NOTICE > This message and any included attachments are from Somerset Medical Center > and are intended only for the addressee. The information contained in this > message is confidential and may contain privileged, confidential, > proprietary and/or trade secret information entitled to protection and/or > exemption from disclosure under applicable law. Unauthorized forwarding, > printing, copying, distribution, or use of such information is strictly > prohibited and may be unlawful. If you are not the addressee, please > promptly delete this message and notify the sender of the delivery error by > e-mail or you may call Somerset Medical Center's computer Help Desk at > 908-685-2200, > ext. 4050. > > Be sure to visit Somerset Medical Center's Web site - > www.somersetmedicalcenter.com - for the most up-to-date news, event > listings, health information and more. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > ------------------------------------------------------------------------- > This message was secured by ZixCorp(R). > > > CONFIDENTIALITY NOTICE > This message and any included attachments are from Somerset Medical Center > and are intended only for the addressee. The information contained in this > message is confidential and may contain privileged, confidential, > proprietary and/or trade secret information entitled to protection and/or > exemption from disclosure under applicable law. Unauthorized forwarding, > printing, copying, distribution, or use of such information is strictly > prohibited and may be unlawful. If you are not the addressee, please > promptly delete this message and notify the sender of the delivery error > by e-mail or you may call Somerset Medical Center's computer Help Desk > at 908-685-2200, ext. 4050. > > Be sure to visit Somerset Medical Center's Web site - > www.somersetmedicalcenter.com - for the most up-to-date news, > event listings, health information and more. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet