Susan, I have stained 60um fixed sections on plus slides without any problem. I free float the sections in a multi-well plate containing PBS or TBS. When mounting the sections onto the plus slide, I wipe away the excess fluid after EACH section, so that the previous section will not be exposed to the PBS again which results in unequal drying. I also place the slide in a slanted position on the slide tray and allow them to dry overnight. The next morning you could also dry them in a 37 degree oven for a few minutes to help in adherence.
I have had others in the lab mount sections at the same time as I do and have often observed that their sections will flap on the slide during staining. Mine, on the other hand, don't come off of the slide. I can only surmise that this is due to many years of experience in mounting sections and the importance of "wicking" away the excess buffer. Good luck, Tina -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Michael, Susan Sent: Friday, July 29, 2011 8:12 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Cresyl violet stain on 50 um mouse brain sections I am having trouble keeping my sections on the slides when I stain with cresyl violet. These are fixed frozen sections, 50 um, dried overnight on plus slides, overnight in 1 to 1 alcohol/chloroform, then rehydrated through 100, 95 ETOHs then to water. Into the cresyl violet solution, then 100% to 95% ETOH. Is it the water? Is it the slides? Any suggestions? Susan _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet