Have you tried the "cell safe" cassette insert from Ted Pella? http://www.tedpella.com/embed_html/27141.htm#anchor27165
Cellsafe(tm) Biopsy Cassettes (white) shown open and closed & inserted into a regular tissue cassette. Cellsafe(tm) Biopsy Cassettes The Cellsafe(tm) is designed to contain small tissue fragments and biopsies during tissue processing. The Cellsafe(tm) Biopsy Insert with a hinged cover fits into a regular cassette. Top and bottom consist of extra fine nylon mesh preventing small tissues from getting lost during specimen preparation, keeping them safe. The plastic frame and nylon mesh are suitable for use in common tissue processing reagents including Formaldehyde solutions, Bouin's Fluid, Alcohol solutions, Xylene, Chloroform, Citrus based clearing agents and Paraffin wax (melting point up to 65°C). The plastic frame is made from the same material widely used in the manufacture of plastic processing cassettes. If you are using any unusual reagents it is suggested that you carry out preliminary chemical compatibility tests before using the Cellsafe(tm) to contain tissue samples. The Cellsafe(tm) is designed for single use only. Interior dimesions of a regular cassette are: 30 x 26 x 4.92mm H. Exterior dimensions of the Cellsafe Biopsy Cassettes are: 27.5 x 25 x 4.57mm H. Item # 21765, bag of 100 $40.85 Lyn Stadler Histology Technician Department of Histopathology Cleveland Biolabs, Inc. 73 High Street Buffalo, NY 14203 -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of histonet-requ...@lists.utsouthwestern.edu Sent: Wednesday, August 10, 2011 1:07 PM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 93, Issue 13 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-requ...@lists.utsouthwestern.edu You can reach the person managing the list at histonet-ow...@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. RE: TFE-3 (Bernice Frederick) 2. What's your favorite phospho-histone H3 antibody for IHC? (Connolly, Brett M) 3. Re: Manual embedding (Collette, Nicole M.) 4. Fern tissue loss during in situ hybridization (Cordle, Angela R) 5. Re: Fern tissue loss during in situ hybridization (Rene J Buesa) 6. Sterilized cassette (Brandi Farris) 7. Re:2 (lynn13...@aol.com) 8. RE: Sterilized cassette (Mike Pence) 9. Cell block H&E staining (Dessoye, Michael J) 10. Re: Cell block H&E staining (Rene J Buesa) 11. Histotech needed in San Diego. Can you help? (Pam Barker) 12. CYTOLOGIST JOB OPENING PHILLY (rmweber...@comcast.net) 13. GROSSING SPECIMENS (Sara Baldwin/mhhcc.org) 14. Cyrostat Oppinions (McMahon, Loralee A) ---------------------------------------------------------------------- Message: 1 Date: Tue, 9 Aug 2011 18:05:51 +0000 From: Bernice Frederick <b-freder...@northwestern.edu> Subject: [Histonet] RE: TFE-3 To: "Settembre, Dana" <sette...@umdnj.edu>, "'Houston, Ronald'" <ronald.hous...@nationwidechildrens.org>, "'histonet@lists.utsouthwestern.edu'" <histonet@lists.utsouthwestern.edu> Message-ID: <62c639732d3f274daced033ebdf6adaf1e12d...@evcspmbx3.ads.northwestern.edu> Content-Type: text/plain; charset="us-ascii" Same here. -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Settembre, Dana Sent: Tuesday, August 09, 2011 11:05 AM To: 'Houston, Ronald'; 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] RE: TFE-3 We use TFE-3 (p16) from Santa Cruz, made in Goat. Cat.# SC-5958 Dana Settembre University Hospital - UMDNJ Newark, NJ -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Houston, Ronald Sent: Tuesday, August 09, 2011 12:02 PM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] TFE-3 Is anyone using TFE-3? If so I would appreciate which clone is being used most. Thanks Ronnie Houston, MS HT(ASCP)QIHC Anatomic Pathology Manager ChildLab, a Division of Nationwide Children's Hospital www.childlab.com 700 Children's Drive Columbus, OH 43205 (P) 614-722-5450 (F) 614-722-2899 ronald.hous...@nationwidechildrens.org<mailto:ronald.hous...@nationwidechildrens.org> www.NationwideChildrens.org<http://www.NationwideChildrens.org> "One person with passion is better than forty people merely interested." ~ E.M. Forster ----------------------------------------- Confidentiality Notice: The following mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. The recipient is responsible to maintain the confidentiality of this information and to use the information only for authorized purposes. If you are not the intended recipient (or authorized to receive information for the intended recipient), you are hereby notified that any review, use, disclosure, distribution, copying, printing, or action taken in reliance on the contents of this e-mail is strictly prohibited. If you have received this communication in error, please notify us immediately by reply e-mail and destroy all copies of the original message. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 2 Date: Tue, 9 Aug 2011 15:09:47 -0400 From: "Connolly, Brett M" <brett_conno...@merck.com> Subject: [Histonet] What's your favorite phospho-histone H3 antibody for IHC? To: "histonet@lists.utsouthwestern.edu" <histonet@lists.utsouthwestern.edu> Message-ID: <c01c35b84dcdce49bc60867e87f1c8fe3370a9a...@usctmxp51015.merck.com> Content-Type: text/plain; charset="us-ascii" ....p(Ser10) or p(Ser28) ? And have you found pHH3 (Ser28) to be more M-phase specific? Thanks, Brett Brett M. Connolly, Ph.D. Molecular Imaging Team Leader Merck & Co., Inc. PO Box 4, WP-44K West Point, PA 19486 brett_conno...@merck.com T- 215-652-2501 F- 215-993-6803 Notice: This e-mail message, together with any attachments, contains information of Merck & Co., Inc. (One Merck Drive, Whitehouse Station, New Jersey, USA 08889), and/or its affiliates Direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. ------------------------------ Message: 3 Date: Tue, 9 Aug 2011 12:10:24 -0700 From: "Collette, Nicole M." <collet...@llnl.gov> Subject: Re: [Histonet] Manual embedding To: "Histonet@lists.utsouthwestern.edu" <Histonet@lists.utsouthwestern.edu> Message-ID: <ca66d430.70a2%collet...@llnl.gov> Content-Type: text/plain; charset="us-ascii" Hi, All, I just saw this question and the responses, thought I would add my own solution to the mix. I do manual embedding, I use a hyb oven for my infiltrating/embedding station (like for hybridizing Southern or Northern blots- who does that anymore?). I have taken out the rotating wheel for spinning bottles, and line the tray at the bottom with foil. Temp control works very well. I have several Wheaton staining boxes (the kind that come with glass inserts for staining 20 slides) that I use for my wax changes for infiltrating, and a couple of metal beakers I use for pouring into molds. I have a little real estate left inside the oven (it's probably around 18"x 18" square area) to heat my molds for pouring, and I have a metal heat block in there that you would use for Eppendorf tubes as my forceps warmer. It works pretty well, but does take a long time to heat up those boxes of wax, so I need to plan ahead by about 3 hours. When I pour the molds, I have the oven in front of a drawer under the bench, I line an extra cabinet shelf with foil and lay it over the open drawer to give me some bench space, pour my molds inside the oven and transfer it to the foil-lined shelf to cool so I can move it without the specimen shifting. Then I transfer to a photo tray and cool in the fridge for a couple of hours before releasing the molds. With the door of the hyb oven open I have to embed in shifts and let the molds heat up again, but it works OK. At least it's all contained in one unit. It's hard to do histology in a molecular lab ;) Hope this helps to give a do-it-your-selfer some ideas. Sincerely, Nicole Collette LLNL On 8/2/11 7:53 PM, "Scott Parker" <spar...@vt.edu> wrote: > Dear Histonetters: > > I am interested in acquiring a pitcher and heating jacket for melting and > pouring paraffin during manual embedding. My work is relatively low volume > and in a university research lab setting so I am trying to avoid purchasing > an expensive embedding station. Can anyone recommend an honest supplier of > used histology equipment that might be able to provide me with this item? > > Thank you for your expertise! > > Scott L. Parker > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 4 Date: Tue, 9 Aug 2011 20:46:03 +0000 From: "Cordle, Angela R" <angela-cor...@uiowa.edu> Subject: [Histonet] Fern tissue loss during in situ hybridization To: "histonet@lists.utsouthwestern.edu" <histonet@lists.utsouthwestern.edu> Message-ID: <32f6f3c172009d44a3b5e6702b54dbf602a77...@itsnt443.iowa.uiowa.edu> Content-Type: text/plain; charset="Windows-1252" Dear Histonet members, I?m having problems with tissue loss during in situ hybridization (ISH) with fern tissue. The fern in question is Ceratopteris richardii, and the tissue (tissue does not contain spores) has been fixed in FAA, dehydrated and embedded in Paraplast plus. Sections are 7 um, and mounted on Probe on Plus slides. I?m not an expert at this process, but I?ve done a fair amount of embedding, sectioning, and staining of various tissues (on Haupt?s coated slies), and I?ve had success with Arabidpsis ISH. I?ll outline the relevant and troubleshooting procedures that I?ve gone through here: 1. Varied the fixation times and methods. Nothing seems to make a difference in the retention of the tissue on the slides. 2. Tried a brand new box of Superfrost Plus slides (the ProbeOn Plus slides are kind-of old), but the superfrost slides actually seemed to provide worse tissue retention than the ProbeOn Plus slides. Arabidopsis tissue prepared in parallel was fine on both slides. 3. For mounting, I float tissue on 37?C DEPC H2O in a waterbath that is free from lotions, or any other substance that I am aware of that can cause problems with tissue adherence. I remove the slides with the newly adhered sections completely vertically. Again, Arabidopsis sections mounted in parallel do not fall off slides in subsequent procedures. 4. I?ve paid a (perhaps) compulsive amount of time making sure that there are no blebs or bubbles of water underneath sections after mounting. Additionally, slides are dried for 1-2 hours (vertically) before baking them at 48?C. I have found that baking the slides for 2 days, rather than 1, slightly increases the tissue retention for the fern, but not enough for quality in situ results. 5. Varied the time and temperature of the proteinase K treatment, but the tissue seems to be falling off the slides before this step anyway. It is gone after this step for sure, regardless of the time or temperature. 5. Taken a great amount of care to ensure that there is no residual tert butyl alcohol (we dehydrate through an ethanol series them into 100% TBA before transitioning to Paraplast) in the paraplast before embedding and sectioning. Arabidopsis tissue embedded in the same blocks has performed perfectly fine in ISH. Okay. So, here are my specific questions: Could it be that this fern tissue is not sufficiently negatively charged that the tissue will not adhere to any polyL lysine coated slide? What the beep should I try next? In advance, thank you all so much for taking the time to help me out with this frustrating problem! --Angie ------------------------------ Message: 5 Date: Tue, 9 Aug 2011 13:59:18 -0700 (PDT) From: Rene J Buesa <rjbu...@yahoo.com> Subject: Re: [Histonet] Fern tissue loss during in situ hybridization To: "histonet@lists.utsouthwestern.edu" <histonet@lists.utsouthwestern.edu>, Angela RCordle <angela-cor...@uiowa.edu> Message-ID: <1312923558.59874.yahoomailclas...@web65711.mail.ac4.yahoo.com> Content-Type: text/plain; charset=utf-8 I think you should try to find out about the cellulose contents of the fern you are working with, and compare it to that of Arabidopsis. If the fern (Ceratopteris) contains more cellulose, I think this is the cause of your problem. If that is the case I think you should try to use a paraffin wax of higher melting point (63-65??C) to assure an infiltration more compatible with the cellulose. Also make sure that the infiltration is optimal. Finally if you can use thinner sections (<7 ??m) that could help also. Ren?? J. --- On Tue, 8/9/11, Cordle, Angela R <angela-cor...@uiowa.edu> wrote: From: Cordle, Angela R <angela-cor...@uiowa.edu> Subject: [Histonet] Fern tissue loss during in situ hybridization To: "histonet@lists.utsouthwestern.edu" <histonet@lists.utsouthwestern.edu> Date: Tuesday, August 9, 2011, 4:46 PM Dear Histonet members, I???m having problems with tissue loss during in situ hybridization (ISH) with fern tissue. The fern in question is Ceratopteris richardii, and the tissue (tissue does not contain spores) has been fixed in FAA, dehydrated and embedded in Paraplast plus. Sections are 7 um, and mounted on Probe on Plus slides. I???m not an expert at this process, but I???ve done a fair amount of embedding, sectioning, and staining of various tissues (on Haupt???s coated slies), and I???ve had success with Arabidpsis ISH. I???ll outline the relevant and troubleshooting procedures that I???ve gone through here: 1. Varied the fixation times and methods. Nothing seems to make a difference in the retention of the tissue on the slides. 2. Tried a brand new box of Superfrost Plus slides (the ProbeOn Plus slides are kind-of old), but the superfrost slides actually seemed to provide worse tissue retention than the ProbeOn Plus slides. Arabidopsis tissue prepared in parallel was fine on both slides. 3. For mounting, I float tissue on 37??C DEPC H2O in a waterbath that is free from lotions, or any other substance that I am aware of that can cause problems with tissue adherence. I remove the slides with the newly adhered sections completely vertically. Again, Arabidopsis sections mounted in parallel do not fall off slides in subsequent procedures. 4. I???ve paid a (perhaps) compulsive amount of time making sure that there are no blebs or bubbles of water underneath sections after mounting. Additionally, slides are dried for 1-2 hours (vertically) before baking them at 48??C. I have found that baking the slides for 2 days, rather than 1, slightly increases the tissue retention for the fern, but not enough for quality in situ results. 5. Varied the time and temperature of the proteinase K treatment, but the tissue seems to be falling off the slides before this step anyway. It is gone after this step for sure, regardless of the time or temperature. 5. Taken a great amount of care to ensure that there is no residual tert butyl alcohol (we dehydrate through an ethanol series them into 100% TBA before transitioning to Paraplast) in the paraplast before embedding and sectioning. Arabidopsis tissue embedded in the same blocks has performed perfectly fine in ISH. Okay. So, here are my specific questions: Could it be that this fern tissue is not sufficiently negatively charged that the tissue will not adhere to any polyL lysine coated slide? What the beep should I try next? In advance, thank you all so much for taking the time to help me out with this frustrating problem!?? ?? --Angie _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 6 Date: Tue, 9 Aug 2011 19:46:25 -0500 From: Brandi Farris <brandihi...@hotmail.com> Subject: [Histonet] Sterilized cassette To: <histonet@lists.utsouthwestern.edu> Message-ID: <blu163-w32ba3a7a7396d56df1186b4...@phx.gbl> Content-Type: text/plain; charset="iso-8859-1" My OR department is wanting a small biopsy cassette that they can sterilize, be in the sterile field and place small biopsy specimens directly into a cassette before placing in formalin. We've had a problem with small biopsies dissolving/disappearing in telfa pads. We can't seem to find a cassette that the manufacture approves to be sterilized. I have a sample of a metal biopsy cassette, but the lid closure is questionable. I'm having trouble thinking "out of the box" on this problem. I've never had an OR request this before. Any tips would be greatly appreciated! Thank you, Brandi Capital Region Medical Center Jefferson City, MO ------------------------------ Message: 7 Date: Wed, 10 Aug 2011 03:58:46 -0400 (EDT) From: lynn13...@aol.com Subject: [Histonet] Re:2 To: gmei...@pima.edu, goffma...@msn.com, hi...@nsh.org, histonet@lists.utsouthwestern.edu, bookn...@cox.net, i...@casablancagardens.com Message-ID: <8ce254a777897b7-15c0-2c...@webmail-d108.sysops.aol.com> Content-Type: text/plain; charset="us-ascii"; format=flowed Hello! Tell me your feelings after trying this stuff!. http://aldeiadovale.com.br/com.page.php?pyhID=86tj5 ------------------------------ Message: 8 Date: Wed, 10 Aug 2011 07:57:27 -0500 From: "Mike Pence" <mpe...@grhs.net> Subject: RE: [Histonet] Sterilized cassette To: "Brandi Farris" <brandihi...@hotmail.com>, <histonet@lists.utsouthwestern.edu> Message-ID: <661949901a768e4f9cc16d8af8f2838c03974...@is-e2k3.grhs.net> Content-Type: text/plain; charset="US-ASCII" My question is this. Who will open the cassette and dictate the specimen and what if the specimen does not make it thru processing? Who is responsible then for it missing? -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Brandi Farris Sent: Tuesday, August 09, 2011 7:46 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Sterilized cassette My OR department is wanting a small biopsy cassette that they can sterilize, be in the sterile field and place small biopsy specimens directly into a cassette before placing in formalin. We've had a problem with small biopsies dissolving/disappearing in telfa pads. We can't seem to find a cassette that the manufacture approves to be sterilized. I have a sample of a metal biopsy cassette, but the lid closure is questionable. I'm having trouble thinking "out of the box" on this problem. I've never had an OR request this before. Any tips would be greatly appreciated! Thank you, Brandi Capital Region Medical Center Jefferson City, MO _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 9 Date: Wed, 10 Aug 2011 10:35:01 -0400 From: "Dessoye, Michael J" <mjdess...@wvhcs.org> Subject: [Histonet] Cell block H&E staining To: <histonet@lists.utsouthwestern.edu> Message-ID: <e2547e1cd0ee324488a2940994571efa0401f...@wvhcs-exchange.wvhcs.com> Content-Type: text/plain; charset="iso-8859-1" Hello all, I'm interested to see what staining protocol folks are using for H&E staining of cell blocks. Lately we've been getting varying light and dark staining on cell blocks only. Currently they are run on our standard H&E protocol that we use for all tissues. Does anyone use a separate protocol for cell blocks? Thanks! Mike Michael J. Dessoye, M.S. | Histology Supervisor | Wyoming Valley Health Care System | mjdess...@wvhcs.org <mailto:mjdess...@wvhcs.org> | 575 N. River Street | Wilkes Barre, PA 18764 | Tel: 570-552-1485 | Fax: 570-552-1526 _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you have received this email in error please notify the originator of the message. This footer also confirms that this email message has been scanned for the presence of computer viruses. Any views expressed in this message are those of the individual sender, except where the sender specifies and with authority, states them to be the views of Wyoming Valley Health Care System. Scanning of this message and addition of this footer is performed by Websense Email Security software in conjunction with virus detection software. ------------------------------ Message: 10 Date: Wed, 10 Aug 2011 07:38:17 -0700 (PDT) From: Rene J Buesa <rjbu...@yahoo.com> Subject: Re: [Histonet] Cell block H&E staining To: histonet@lists.utsouthwestern.edu, Michael JDessoye <mjdess...@wvhcs.org> Message-ID: <1312987097.19039.yahoomailclas...@web65707.mail.ac4.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 The? staining variability you are obtaining on cell blocks is caused by the medium you are preparing them in. We always used the same H&E protocol and I do not think you will solve the problems by changing the H&E protocol, but by modifying the way you prepare the cell block. Ren? J. --- On Wed, 8/10/11, Dessoye, Michael J <mjdess...@wvhcs.org> wrote: From: Dessoye, Michael J <mjdess...@wvhcs.org> Subject: [Histonet] Cell block H&E staining To: histonet@lists.utsouthwestern.edu Date: Wednesday, August 10, 2011, 10:35 AM Hello all, I'm interested to see what staining protocol folks are using for H&E staining of cell blocks.? Lately we've been getting varying light and dark staining on cell blocks only.? Currently they are run on our standard H&E protocol that we use for all tissues.? Does anyone use a separate protocol for cell blocks? Thanks! Mike Michael J. Dessoye, M.S. | Histology Supervisor | Wyoming Valley Health Care System | mjdess...@wvhcs.org <mailto:mjdess...@wvhcs.org>? | 575 N. River Street | Wilkes Barre, PA 18764 | Tel: 570-552-1485 | Fax: 570-552-1526 _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you have received this email in error please notify the originator of the message. This footer also confirms that this email message has been scanned for the presence of computer viruses. Any views expressed in this message are those of the individual sender, except where the sender specifies and with authority, states them to be the views of Wyoming Valley Health Care System. Scanning of this message and addition of this footer is performed by Websense Email Security software in conjunction with virus detection software. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 11 Date: Wed, 10 Aug 2011 11:02:18 -0400 From: "Pam Barker" <rel...@earthlink.net> Subject: [Histonet] Histotech needed in San Diego. Can you help? To: "'Histonet'" <histonet@lists.utsouthwestern.edu> Message-ID: <10BE35346E43450B80E74E70B6469EBE@ownerf1abaad51> Content-Type: text/plain; charset="iso-8859-1" Hi Histonetters!! How are you? I have a new histology position and I need your help. I am currently working with a leading edge cancer diagnostic laboratory in the San Diego area that is in need of a histotechnologist with strong immunohistochemistry experience. This is a permanent full time position and the schedule is 9a-530p Tuesday-Saturday. The client offers a great environment, a great crew to work with, excellent salary, great benefits and a generous relocation package. ASCP HT or HTL and a B.S. degree in a science related major are required. QIHC is a plus. My question is do you know of anyone who might be interested in this position? I really appreciate you taking the time to read this e-mail and it means a lot to me when you take the time to refer your friends and coworkers so to show my appreciation I would like to offer you a 500.00 referral fee for anyone you refer to me that I place. So if you think you or someone you know might be interested please contact me. I can be reached at 866-607-3542 or <mailto:rel...@earthlink.net> rel...@earthlink.net Thanks-Pam Thank You! Pam Barker President RELIA Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 E-mail: <mailto:rel...@earthlink.net> rel...@earthlink.net <http://www.facebook.comPamBarkerRELIA> www.facebook.comPamBarkerRELIA <http://www.linkedin.com/reliasolutions> www.linkedin.com/reliasolutions <http://www.myspace.com/pamatrelia> www.myspace.com/pamatrelia <http://www.twitter.com/pamatrelia> www.twitter.com/pamatrelia ------------------------------ Message: 12 Date: Wed, 10 Aug 2011 15:47:29 +0000 (UTC) From: rmweber...@comcast.net Subject: [Histonet] CYTOLOGIST JOB OPENING PHILLY To: histonet <histonet@lists.utsouthwestern.edu> Message-ID: <1743836975.17768.1312991249090.javamail.r...@sz0046a.westchester.pa.mail.comcast.net> Content-Type: text/plain; charset=utf-8 We have a part time job evening??opening for a cytologist to read urine cytology.?? The job is located in the Northeast Philadelphia area in a urology practice.?? Eligible candidates?? must have at least 3 years experience.?? Candidates can fax resumes to 215 947-2015 attention laboratory or call 215 947-4480. Thank you, ------------------------------ Message: 13 Date: Wed, 10 Aug 2011 11:47:42 -0400 From: "Sara Baldwin/mhhcc.org" <sbald...@mhhcc.org> Subject: [Histonet] GROSSING SPECIMENS To: "'Histonet'" <histonet@lists.utsouthwestern.edu> Message-ID: <OFF3D6630D.A0DBBA98-ON852578E8.0056C3CB-852578E8.0056C3D0@LocalDomain> Content-Type: text/plain; charset=ISO-8859-1 Histonetters If my Histo tech has an associates degree with enough chemistry courses can they gross the small specimens?? Acording to the new CAP, JOINT COMMISSION nad CLIAA regs?? Thanks Pathology Supervisor S. Kathy Baldwin, SCT (ASCP) Memorial Hospital and Health Care Center sbald...@mhhcc.org Ph 812-482-0210, 0216, Fax 812-482-0232, Pager 812-481-0897, Cell 812-887-3357 Confidential information, Authorized use only. ------------------------------ Message: 14 Date: Wed, 10 Aug 2011 11:56:33 -0400 From: "McMahon, Loralee A" <loralee_mcma...@urmc.rochester.edu> Subject: [Histonet] Cyrostat Oppinions To: "Histonet@lists.utsouthwestern.edu" <Histonet@lists.utsouthwestern.edu> Message-ID: <c27aa2a01cef31469813089e226f582e055f27b...@urmcms7.urmc-sh.rochester.edu> Content-Type: text/plain; charset="iso-8859-1" Hi We are in the process of getting a new cyrostat and would like some opinions. We are looking at the ThermoFisher HM550. Please feel free to contact me offline if you like. Thank you in advance. Loralee McMahon, HTL (ASCP) Immunohistochemistry Supervisor Strong Memorial Hospital Department of Surgical Pathology (585) 275-7210 ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 93, Issue 13 **************************************** This communication may contain privileged information. It is intended solely for the use of the addressee. If you are not the intended recipient, you are strictly prohibited from disclosing, copying, distributing or using any of this information. If you received this communication in error, please contact the sender immediately and destroy the material in its entirety, whether electronic or hard copy. This communication may contain nonpublic information about individuals and businesses subject to the restrictions of the Gramm-Leach-Bliley Act. You may not directly or indirectly reuse or redisclose such information for any purpose other than to provide the services for which you are receiving the information. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet