Hi all, I'm using the Churukian method posted by Gayle Callis a few years ago and I have encountered a strange problem. The lipid stains fine, and the Gill's hematoxylin looks good before I coverslip with Vectashield Hard Set aqueous mounting medium. But within a matter of minutes, the hematoxylin leaches out. I popped off the coverslip and tried again with Mayer's hematoxylin with the same results. I have used Vectashield Hard Set before, in fact I pulled a 5 year old control that still looks great. Does anyone have any ideas?
Denise Crowley Histology Facility Manager Koch Center for Integrative Cancer Research Massachusetts Institute of Technology 500 Main St. 76-182 Cambridge MA 02139 617-258-8183 dencr...@mit.edu _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
