I know different people do lot to lot checks in various ways. We use concentrated antibodies, and so we like to do a 'sandwich' titer check on the new lot using our usual tissue control tissue. For example, if the last CD20 lot was at 1:100 we would check the new lot at 1:50, 1:100, 1:200 using tonsil tissues & our standard SOPs. I did have 1 inspector tell me they thought that was unnecessary. It's our preference to do 3 titers & assess the maximum performance in one fell swoop. Our detection lot to lot check is completely different. We check 8-10 different antibodies using our standard control tissue with the new detection lot. we use antibodies with a variety of cellular expressions to assess cytoplasmic, membranous & nuclear expression. We make sure to include some of our 'fussy' antibodies. Of course we have SOPs & forms to go along with this. Call me crazy - I prefer to think of it as meticulous & dedicated! We have had a couple of problems with detections over the years. Just my 2 cents.
Patti Ann Loykasek HTL, QIHC PhenoPath Laboratories On Aug 29, 2011, at 9:06 AM, Mary Helie wrote: > Good Afternoon > > I would like to get an idea of how many slides people are running for lot to > lot antibody and detection testing. For example would a tonsil with good > internal negative and positive elements be good enough to validate a new lot > of LCA? > > Thank you > Mary > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > This e-mail message, including any attachments, is for the sole use of the intended recipients and may contain privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by e-mail and destroy all copies of the original message, or you may call PhenoPath Laboratories, Seattle, WA U.S.A. at (206) 374-9000. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
