We use Rapid Cal Immuno (formic acid based) from BBC Biocemical out of Washington. Works great and does not compromise IHC as name suggests!
Dorothy Webb -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of histonet-requ...@lists.utsouthwestern.edu Sent: Friday, September 09, 2011 12:05 PM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 94, Issue 9 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-requ...@lists.utsouthwestern.edu You can reach the person managing the list at histonet-ow...@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Re: P63 controls (Troutman, Kenneth A) 2. Full Time HT position in Oklahoma City (Gaiser, Marcia) 3. STP420 vs. Peloris (Davide Costanzo) 4. Seeking new position (Charles O) 5. Comment on HT/HTL (anuradha shrivastava) 6. Facebook (Nicole Tatum) 7. thinprep (tahs...@brain.net.pk) 8. I'm so sad for AFIP to close (Weems, Joyce) 9. RE: I'm so sad for AFIP to close (sdys...@mirnarx.com) 10. RE: I'm so sad for AFIP to close (Edwards, Richard E.) 11. Decal for IHC (Laudon, Heather M) 12. RE: I'm so sad for AFIP to close (Shirley A. Powell) 13. Floater Problem (Laurie Colbert) 14. Re: Decal for IHC (Rene J Buesa) 15. RE: Floater Problem (Bartlett, Jeanine (CDC/OID/NCEZID)) 16. RE: I'm so sad for AFIP to close (Kelly Cross) 17. Re: Floater Problem (Kelly Cross) 18. RE: Floater Problem (Bea DeBrosse-Serra) 19. RE: Decal for IHC (Shirley A. Powell) 20. Traveling HistoTech San Francisco Area (Heckford, Karen - SMMC-SF) 21. RE: Floater Problem (Shirley A. Powell) 22. RE: Decal for IHC (Sebree Linda A) ---------------------------------------------------------------------- Message: 1 Date: Thu, 8 Sep 2011 14:16:21 -0500 From: "Troutman, Kenneth A" <ashley.trout...@vanderbilt.edu> Subject: Re: [Histonet] P63 controls To: "Histonet@lists.utsouthwestern.edu" <Histonet@lists.utsouthwestern.edu> Message-ID: <7b310892042da74cb3590053f424cfe61441fce...@its-hcwnem06.ds.vanderbilt.edu> Content-Type: text/plain; charset="us-ascii" We use normal prostate and we have also used skin in the past-they both work well. I would avoid pre-cutting a lot of controls, though; it has a tendency to loose antigenicity. Ashley Troutman BS, HT(ASCP) QIHC Immunohistochemistry Supervisor Vanderbilt University Histopathology 1301 Medical Center Drive TVC 4531 Nashville, TN 37232 Message: 9 Date: Thu, 8 Sep 2011 09:08:52 -0400 From: "Sara Baldwin/mhhcc.org" <sbald...@mhhcc.org> Subject: [Histonet] P63 controls To: <histonet@lists.utsouthwestern.edu> Message-ID: <OF62E3D6D8.5113CF5C-ON85257905.0048390E-85257905.00483911@LocalDomain> Content-Type: text/plain; charset=ISO-8859-1 Hi Histonetters We are having trouble with our control for P63 what is everyone using? Thanks Pathology Supervisor S. Kathy Baldwin, SCT (ASCP) Memorial Hospital and Health Care Center sbald...@mhhcc.org Ph 812-996-0210, 0216, Fax 812-996-0232, Pager 812-481-0897, Cell 812-887-3357 Confidential information, Authorized use only. ------------------------------ Message: 2 Date: Thu, 8 Sep 2011 15:16:48 -0500 From: "Gaiser, Marcia" <marcia_gai...@ssmhc.com> Subject: [Histonet] Full Time HT position in Oklahoma City To: "histonet@lists.utsouthwestern.edu" <histonet@lists.utsouthwestern.edu> Message-ID: <728f817c02110e498d803a7c3b0c62480690a37...@s009-apexm06.ds.ad.ssmhc.com> Content-Type: text/plain; charset="Windows-1252" POSITION REQUIREMENTS: EDUCATION: High school diploma or GED preferred. CERTIFICATION, LICENSURE, BONDING: Certified as an HT or HLT by the American Society of Clinical Pathologists (ASCP) ? or ? other nationally recognized certifying agency acceptable to the Laboratory Director ? or ? experience acceptable to the Laboratory Director. EXPERIENCE: Two years of satisfactory histology experience. SIGN ON BONUS AVAILABLE FOR QUALIFIED CANDIDATES. Outstanding benefits package including generous paid time off. Apply online at www.saintsok.com<http://www.saintsok.com<http://www.saintsok.com/><https://mobile.ssmhc.com/owa/UrlBlockedError.aspx>>, Ad# 10762, or contact Christina Hughes at (405) 272-6105 for more information. Thank you! Christina Hughes HR Recruiter St. Anthony Hospital (405) 272-6105 - phone (405) 272-6781 - fax Confidentiality Notice: This email message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply email and destroy all copies of the original message. _______________________________________________ ------------------------------ Message: 3 Date: Thu, 8 Sep 2011 13:37:12 -0700 From: Davide Costanzo <pathloc...@gmail.com> Subject: [Histonet] STP420 vs. Peloris To: histonet@lists.utsouthwestern.edu Message-ID: <CA+F+RhoC=fsmo4ufjq+vv8bwu+hc3p3dr40x4_2voubni+n...@mail.gmail.com> Content-Type: text/plain; charset=ISO-8859-1 Considering both options. Can any STP users out there give me opinions? Please give reasons, not just "go with" this one. Thanks. ------------------------------ Message: 4 Date: Thu, 8 Sep 2011 17:07:35 -0500 From: Charles O <charleso.o...@gmail.com> Subject: [Histonet] Seeking new position To: histonet@lists.utsouthwestern.edu Message-ID: <CAJvHW2+d1dERKCEDyrJ=UTXufvNVu=t04+=cwsojneatum8...@mail.gmail.com> Content-Type: text/plain; charset=ISO-8859-1 Hi, I am a certified histotech seeking a new position. I have an extensive experience in the general histological technics and in Immunohistochemistry. I am willing to relocate. Thanks, Charles O. ------------------------------ Message: 5 Date: Thu, 8 Sep 2011 20:55:08 -0400 From: anuradha shrivastava <shrivastavaanura...@hotmail.com> Subject: [Histonet] Comment on HT/HTL To: <histonet@lists.utsouthwestern.edu> Message-ID: <snt115-ds1048065f65f786eaeeb14bad...@phx.gbl> Content-Type: text/plain; charset="iso-8859-1" Hi All, I feel so fortunate that I studied Histo in Cobleskill, with my bio background It was perfect. I think the science sub. background is also needed specially anatomy, so that one knows how to embed different tissues. I hope u all will agree with me. Slide submittion should be there in the exam. Anu Shrivastava. M.S , HTL (ASCP) Histology Team Leader, South County Hospital RI ------------------------------ Message: 6 Date: Fri, 9 Sep 2011 09:21:06 -0400 (EDT) From: "Nicole Tatum" <nic...@dlcjax.com> Subject: [Histonet] Facebook To: histonet@lists.utsouthwestern.edu Message-ID: <4934.208.62.167.196.1315574466.squir...@webmail.realpages.com> Content-Type: text/plain;charset=iso-8859-1 Hey histonetters, Ive created a community page for histologist on Facebook. Please join or like Histo-ville. It a page for people to make friends, troubleshoot, or discuss issues. I hope to create a large community of friends. So stop by and say hi. I look forward to meeting fellow techs. Nicole Tatum, HT ASCP ------------------------------ Message: 7 Date: Fri, 9 Sep 2011 18:34:25 +0500 (PKT) From: tahs...@brain.net.pk Subject: [Histonet] thinprep To: histonet@lists.utsouthwestern.edu Cc: c...@skm.org.pk Message-ID: <62815.203.135.35.66.1315575265.squir...@brain.net.pk> Content-Type: text/plain;charset=iso-8859-1 We want to purchase a latest model machine for thin prep/sure path along with an auto stainer for pap.stain. Can you share your experience regarding both types of machines, 1) Which type of system is best 2)which machine will provide an auto stainer 3)which model of the recommended machine is best 4)will they arrange for training of cytotechnologist and pathologist for processing and reportting of specimen 5)which company is providing best after sale service Thanks ------------------------------ Message: 8 Date: Fri, 9 Sep 2011 10:33:42 -0400 From: "Weems, Joyce" <jwe...@sjha.org> Subject: [Histonet] I'm so sad for AFIP to close To: "histonet@lists.utsouthwestern.edu" <histonet@lists.utsouthwestern.edu> Message-ID: <92ad9b20a6c38c4587a9febe3a30e1640827a8b...@chexcms10.one.ads.che.org> Content-Type: text/plain; charset="us-ascii" I never really thought it would happen but it has... http://www.ascp.org/MainMenu/pathologists/AFIP-Closure-Major-Loss-to-Pathology-Community.html Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 678-843-7376 - Phone 678-843-7831 - Fax Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. ------------------------------ Message: 9 Date: Fri, 9 Sep 2011 10:02:43 -0500 From: <sdys...@mirnarx.com> Subject: RE: [Histonet] I'm so sad for AFIP to close To: <jwe...@sjha.org>, <histonet@lists.utsouthwestern.edu> Message-ID: <d957f2a7d21959488c492a2680f9920a296...@svrexch.asuragen.us> Content-Type: text/plain; charset="us-ascii" Wow...that is sad news. Sarah Goebel-Dysart, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce Sent: Friday, September 09, 2011 9:34 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] I'm so sad for AFIP to close I never really thought it would happen but it has... http://www.ascp.org/MainMenu/pathologists/AFIP-Closure-Major-Loss-to-Pat hology-Community.html Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 678-843-7376 - Phone 678-843-7831 - Fax Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 10 Date: Fri, 9 Sep 2011 16:07:32 +0100 From: "Edwards, Richard E." <r...@leicester.ac.uk> Subject: RE: [Histonet] I'm so sad for AFIP to close To: "'sdys...@mirnarx.com'" <sdys...@mirnarx.com>, "jwe...@sjha.org" <jwe...@sjha.org>, "histonet@lists.utsouthwestern.edu" <histonet@lists.utsouthwestern.edu> Message-ID: <7722595275a4dd4fa225b92cdbf174a101a4ec22f...@exc-mbx3.cfs.le.ac.uk> Content-Type: text/plain; charset="us-ascii" Well unfortunately one can't have public/governmental spending cut backs without losing something important along the way. -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of sdys...@mirnarx.com Sent: 09 September 2011 16:03 To: jwe...@sjha.org; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] I'm so sad for AFIP to close Wow...that is sad news. Sarah Goebel-Dysart, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce Sent: Friday, September 09, 2011 9:34 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] I'm so sad for AFIP to close I never really thought it would happen but it has... http://www.ascp.org/MainMenu/pathologists/AFIP-Closure-Major-Loss-to-Pat hology-Community.html Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 678-843-7376 - Phone 678-843-7831 - Fax Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 11 Date: Fri, 9 Sep 2011 15:23:16 +0000 From: "Laudon, Heather M" <hmlau...@gundluth.org> Subject: [Histonet] Decal for IHC To: "histonet@lists.utsouthwestern.edu" <histonet@lists.utsouthwestern.edu> Message-ID: <d5c20a247e19b546bb7bdd59450b37ddd0a...@lxexmb01.gundluth.org> Content-Type: text/plain; charset="iso-8859-1" Can anyone offer a recommendation for a good decalcifier that does not comprimise Immunohistochemistry results...in particular ER/PR? Thanks for your help! Heather Laudon, HTL Histology Technician Surgical Pathology Laboratory Gundersen Lutheran Hospital 1900 South Avenue Lacrosse, WI 54601 Mail Stop: H04-008 (608)775-3139 ------------------------------ Message: 12 Date: Fri, 9 Sep 2011 11:32:47 -0400 From: "Shirley A. Powell" <powell...@mercer.edu> Subject: [Histonet] RE: I'm so sad for AFIP to close To: "Weems, Joyce" <jwe...@sjha.org>, "histonet@lists.utsouthwestern.edu" <histonet@lists.utsouthwestern.edu> Message-ID: <9BF995BC0E47744E9673A41486E24EE238DE9967AE@MERCERMAIL.MercerU.local> Content-Type: text/plain; charset="us-ascii" I know, just breaks your heart. I spent 3 weeks there in 19...... well a long time ago. -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce Sent: Friday, September 09, 2011 10:34 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] I'm so sad for AFIP to close I never really thought it would happen but it has... http://www.ascp.org/MainMenu/pathologists/AFIP-Closure-Major-Loss-to-Pathology-Community.html Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 678-843-7376 - Phone 678-843-7831 - Fax Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 13 Date: Fri, 9 Sep 2011 08:40:48 -0700 From: "Laurie Colbert" <laurie.colb...@huntingtonhospital.com> Subject: [Histonet] Floater Problem To: <histonet@lists.utsouthwestern.edu> Cc: Debra Cobb <debra.c...@dako.com> Message-ID: <57be698966d5c54eae8612e8941d76830ad2b...@exchange3.huntingtonhospital.com> Content-Type: text/plain; charset="us-ascii" I am starting a PI project concerning floaters on slides. I am collecting data and determining whether the floater is in the block or on the slide. If the floater is in the block, it either came from the grossing step or the embedding step. Of course, both the PA and the embedders swear that they clean their forceps and their work surfaces diligently. My problem is in determining where the floater actually came from. Any suggestions?? Thanks, Laurie Colbert ------------------------------ Message: 14 Date: Fri, 9 Sep 2011 08:45:54 -0700 (PDT) From: Rene J Buesa <rjbu...@yahoo.com> Subject: Re: [Histonet] Decal for IHC To: "histonet@lists.utsouthwestern.edu" <histonet@lists.utsouthwestern.edu>, Heather MLaudon <hmlau...@gundluth.org> Message-ID: <1315583154.12504.yahoomailclas...@web65711.mail.ac4.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 EDTA pH 7 Ren? J. --- On Fri, 9/9/11, Laudon, Heather M <hmlau...@gundluth.org> wrote: From: Laudon, Heather M <hmlau...@gundluth.org> Subject: [Histonet] Decal for IHC To: "histonet@lists.utsouthwestern.edu" <histonet@lists.utsouthwestern.edu> Date: Friday, September 9, 2011, 11:23 AM Can anyone offer a recommendation for a good decalcifier that does not comprimise Immunohistochemistry results...in particular ER/PR? Thanks for your help! Heather Laudon, HTL Histology Technician Surgical Pathology Laboratory Gundersen Lutheran Hospital 1900 South Avenue Lacrosse, WI 54601 Mail Stop: H04-008 (608)775-3139 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 15 Date: Fri, 9 Sep 2011 15:45:08 +0000 From: "Bartlett, Jeanine (CDC/OID/NCEZID)" <j...@cdc.gov> Subject: [Histonet] RE: Floater Problem To: Laurie Colbert <laurie.colb...@huntingtonhospital.com>, "histonet@lists.utsouthwestern.edu" <histonet@lists.utsouthwestern.edu> Cc: Debra Cobb <debra.c...@dako.com> Message-ID: <df1cba3d83d9a344a7d6a045188e448416e35...@embx-clft1.cdc.gov> Content-Type: text/plain; charset="us-ascii" I have also heard of the occasional floater coming from the processor and making its way into the paraffin block. Jeanine Bartlett Infectious Diseases Pathology Branch (404) 639-3590 jeanine.bartl...@cdc.hhs.gov -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Laurie Colbert Sent: Friday, September 09, 2011 11:41 AM To: histonet@lists.utsouthwestern.edu Cc: Debra Cobb Subject: [Histonet] Floater Problem I am starting a PI project concerning floaters on slides. I am collecting data and determining whether the floater is in the block or on the slide. If the floater is in the block, it either came from the grossing step or the embedding step. Of course, both the PA and the embedders swear that they clean their forceps and their work surfaces diligently. My problem is in determining where the floater actually came from. Any suggestions?? Thanks, Laurie Colbert _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 16 Date: Fri, 09 Sep 2011 10:49:29 -0500 From: "Kelly Cross" <kcr...@cvm.tamu.edu> Subject: [Histonet] RE: I'm so sad for AFIP to close To: "histonet@lists.utsouthwestern.edu" <histonet@lists.utsouthwestern.edu>, "Shirley A. Powell" <powell...@mercer.edu>, "Joyce Weems" <jwe...@sjha.org> Message-ID: <4e69ef39020000e3000da...@cvm.tamu.edu> Content-Type: text/plain; charset=US-ASCII As a young histotech I felt I had "arrived" when my supervisor assigned me to take care of the AFIP study set years ago! It is sad... Kelly S. Cross B.S., HT (ASCP) Medical Laboratory Supervisor Texas A&M University Veterinary Pathobiology VMA bldg. 207B College Station, TX 77843-4467 Office: 979-862-3658 Lab: 979-845-5149 Fax: 979-862-1147 kcr...@cvm.tamu.edu >>> "Shirley A. Powell" <powell...@mercer.edu> 9/9/2011 10:32 AM >>> I know, just breaks your heart. I spent 3 weeks there in 19...... well a long time ago. -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce Sent: Friday, September 09, 2011 10:34 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] I'm so sad for AFIP to close I never really thought it would happen but it has... http://www.ascp.org/MainMenu/pathologists/AFIP-Closure-Major-Loss-to-Pathology-Community.html Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 678-843-7376 - Phone 678-843-7831 - Fax Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 17 Date: Fri, 09 Sep 2011 10:50:32 -0500 From: "Kelly Cross" <kcr...@cvm.tamu.edu> Subject: Re: [Histonet] Floater Problem To: "Laurie Colbert" <laurie.colb...@huntingtonhospital.com>, <histonet@lists.utsouthwestern.edu> Cc: Debra Cobb <debra.c...@dako.com> Message-ID: <4e69ef78020000e3000da...@cvm.tamu.edu> Content-Type: text/plain; charset=US-ASCII Water bath???? Kelly S. Cross B.S., HT (ASCP) Medical Laboratory Supervisor Texas A&M University Veterinary Pathobiology VMA bldg. 207B College Station, TX 77843-4467 Office: 979-862-3658 Lab: 979-845-5149 Fax: 979-862-1147 kcr...@cvm.tamu.edu >>> "Laurie Colbert" <laurie.colb...@huntingtonhospital.com> 9/9/2011 10:40 AM >>> I am starting a PI project concerning floaters on slides. I am collecting data and determining whether the floater is in the block or on the slide. If the floater is in the block, it either came from the grossing step or the embedding step. Of course, both the PA and the embedders swear that they clean their forceps and their work surfaces diligently. My problem is in determining where the floater actually came from. Any suggestions?? Thanks, Laurie Colbert _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 18 Date: Fri, 9 Sep 2011 08:52:52 -0700 From: Bea DeBrosse-Serra <bdebrosse-se...@isisph.com> Subject: [Histonet] RE: Floater Problem To: "'Laurie Colbert'" <laurie.colb...@huntingtonhospital.com>, "histonet@lists.utsouthwestern.edu" <histonet@lists.utsouthwestern.edu> Cc: Debra Cobb <debra.c...@dako.com> Message-ID: <493caa64f203e14e8823737b9ee0e25f091d4ed...@exchmb01.isis.local> Content-Type: text/plain; charset="us-ascii" Are gloves being worn during the whole process, including slide printing? Beatrice DeBrosse-Serra HT(ASCP)QIHC Isis Pharmaceuticals Antisense Drug Discovery 1896 Rutherford Road Carlsbad, CA 92008 760-603-2371 -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Laurie Colbert Sent: Friday, September 09, 2011 8:41 AM To: histonet@lists.utsouthwestern.edu Cc: Debra Cobb Subject: [Histonet] Floater Problem I am starting a PI project concerning floaters on slides. I am collecting data and determining whether the floater is in the block or on the slide. If the floater is in the block, it either came from the grossing step or the embedding step. Of course, both the PA and the embedders swear that they clean their forceps and their work surfaces diligently. My problem is in determining where the floater actually came from. Any suggestions?? Thanks, Laurie Colbert _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 19 Date: Fri, 9 Sep 2011 11:55:17 -0400 From: "Shirley A. Powell" <powell...@mercer.edu> Subject: [Histonet] RE: Decal for IHC To: "Laudon, Heather M" <hmlau...@gundluth.org>, "histonet@lists.utsouthwestern.edu" <histonet@lists.utsouthwestern.edu> Message-ID: <9BF995BC0E47744E9673A41486E24EE238DE996815@MERCERMAIL.MercerU.local> Content-Type: text/plain; charset="us-ascii" 5% formic acid -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Laudon, Heather M Sent: Friday, September 09, 2011 11:23 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Decal for IHC Can anyone offer a recommendation for a good decalcifier that does not comprimise Immunohistochemistry results...in particular ER/PR? Thanks for your help! Heather Laudon, HTL Histology Technician Surgical Pathology Laboratory Gundersen Lutheran Hospital 1900 South Avenue Lacrosse, WI 54601 Mail Stop: H04-008 (608)775-3139 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 20 Date: Fri, 9 Sep 2011 08:55:29 -0700 From: "Heckford, Karen - SMMC-SF" <karen.heckf...@chw.edu> Subject: [Histonet] Traveling HistoTech San Francisco Area To: <histonet@lists.utsouthwestern.edu> Message-ID: <9ecf174e7da83046be6ebfde009e28a388c...@chw-msg-301.chw.edu> Content-Type: text/plain; charset="us-ascii" Hello, I am going to need a ASCP certified Histotech to work for me for about 1.5-2 weeks in the next 2-4 weeks. If you now of a temp. service or someone interested. Call me and leave a message and will get back to you. Karen Heckford HT ASCP CE Lead Histology Technician St. Mary's Medical Center 450 Stanyan St. San Francisco, Ca. 94117 415-668-1000 ext. 6167 Caution: This email message, including all content and attachments, is CONFIDENTIAL and may be of a nature that is LEGALLY PRIVILEGED. The information contained in this email message is intended only for the use of the recipient(s) named above. If the reader of this message is not the intended recipient or an agent responsible for delivering it to the intended recipient, you have received this document in error. Any further review, dissemination, distribution, or copying of this message is strictly prohibited. If you have received this communication in error, please notify us immediately by reply email. Thank you." ------------------------------ Message: 21 Date: Fri, 9 Sep 2011 11:59:45 -0400 From: "Shirley A. Powell" <powell...@mercer.edu> Subject: [Histonet] RE: Floater Problem To: Laurie Colbert <laurie.colb...@huntingtonhospital.com>, "histonet@lists.utsouthwestern.edu" <histonet@lists.utsouthwestern.edu> Cc: Debra Cobb <debra.c...@dako.com> Message-ID: <9BF995BC0E47744E9673A41486E24EE238DE99682D@MERCERMAIL.MercerU.local> Content-Type: text/plain; charset="us-ascii" There is a good article in the JOH, June 2011, Vol 34, Number 2, Gadgets for "Floaters" prevention in the Histopathology Laboratory by Izak Dimenstein that addresses ways to help prevent them. He discusses floater sources in the discussion. Shirley -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Laurie Colbert Sent: Friday, September 09, 2011 11:41 AM To: histonet@lists.utsouthwestern.edu Cc: Debra Cobb Subject: [Histonet] Floater Problem I am starting a PI project concerning floaters on slides. I am collecting data and determining whether the floater is in the block or on the slide. If the floater is in the block, it either came from the grossing step or the embedding step. Of course, both the PA and the embedders swear that they clean their forceps and their work surfaces diligently. My problem is in determining where the floater actually came from. Any suggestions?? Thanks, Laurie Colbert _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 22 Date: Fri, 9 Sep 2011 11:26:56 -0500 From: "Sebree Linda A" <lseb...@uwhealth.org> Subject: RE: [Histonet] Decal for IHC To: "Laudon, Heather M" <hmlau...@gundluth.org>, <histonet@lists.utsouthwestern.edu> Message-ID: <f0b9fd4b28c80e43996ffd4408b198aea5b...@uwhc-mail3.uwhis.hosp.wisc.edu> Content-Type: text/plain; charset="us-ascii" Hi Heather, Whatever we use here, if you remember, doesn't compromise IHC results, even for ER/PR. Jamie says its Protocol decalcifier B. -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Laudon, Heather M Sent: Friday, September 09, 2011 10:23 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Decal for IHC Can anyone offer a recommendation for a good decalcifier that does not comprimise Immunohistochemistry results...in particular ER/PR? Thanks for your help! Heather Laudon, HTL Histology Technician Surgical Pathology Laboratory Gundersen Lutheran Hospital 1900 South Avenue Lacrosse, WI 54601 Mail Stop: H04-008 (608)775-3139 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 94, Issue 9 *************************************** This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. 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