I suspect it will be somewhere between rather ugly to totally worthless,
more likely the latter.
The only was to know is to find out for yourself. Thaw, rinse multiple
times in buffer, osmicate in buffer, rinse, dehydrate, clear and embed
in your favorite epoxy.
Good luck.
Geoff
On 9/13/2011 12:17 PM, Daphne Gill wrote:
Hi
I'd like to try some TEM work on rat brain hippocampus. However, the
tissue is less than ideal. Does anyone have any information/insight on
using old (harvested ~2 years ago) tissue that has been subjected to 20
minutes in sodium sulfide (0.4%), fixed using 30% sucrose in 10% BNF for
48 hours, and then frozen?
Thanks very much for any help you can give me.
Daphne
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Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
675 Hoes Lane, Piscataway, NJ 08854
voice: (732)-235-4583
mcaul...@umdnj.edu
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