We use ProPar on our stainer. We rotate our 4 100% alcohols that are after eosin after every 3rd rack and we have stopped the leaching of eosin in our last ProPars.
Not a big fan of exposing my staff to xylene unless I absolutely have to. Jen Campbell On Thu, Sep 15, 2011 at 9:44 AM, <sdys...@mirnarx.com> wrote: > This happened to me several months ago. Our lab always has high humidity. > If you are going to continue to use clear rite with humidity in the air, I > would change it everyday. Also, change out your 100% because it's > inevitably getting water in it too. I just switched my whole lab back to > xylene...at least you can see the water in it =) > Good Luck > > Sarah Goebel-Dysart, BA, HT(ASCP) > Histotechnologist > Mirna Therapeutics > 2150 Woodward Street > Suite 100 > Austin, Texas 78744 > (512)901-0900 ext. 6912 > > > -----Original Message----- > From: histonet-boun...@lists.utsouthwestern.edu [mailto: > histonet-boun...@lists.utsouthwestern.edu] On Behalf Of anuradha > shrivastava > Sent: Wednesday, September 14, 2011 9:35 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] H&E > > Hello everybody, > Can anyone help me, In Leica autostainer we have after eosin 3 100% series, > clear-rite/100% and then 3 clear rites. I observed that during humid days > eosin was leaching . I replaced 2 clear rite after 50 :50 > Abs/cler. with xylene. The slides were fine after wards. My question is , > is it ok to keep the last clear rite or I should change that too to xylene. > We had never used xylene before in the stainer. The coverslipper’s bath is > filled with the clear rite.( xylene substitute), Please give your expert > advice. Thanks . > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > -- Jen Campbell, HT(ASCP) Supervisor of Technical Services Muhlbauer Dermatopathology Laboratory 61 Monroe Avenue, Ste B Pittsford NY 14534 P: 585.586.5166 F: 585.586.3137 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet