Dear all, Apologies for the trouble. I am working on fresh human cancer cells on slides here in Singapore. I am trying to visualize the slides concurrently using both immunofluorescence and a more conventional nuclear stain used in pathology laboratories.
I have tried Diffquik and Giemsa, but both seem to quench the fluorescence signal. I have tried a GFP-expressing cell line, and the same quenching seems to occur as well. I was wondering if anyone has ever needed to do similar work, and if so, what sort of solutions (bad pun) were adopted? Thank you! Min-Han _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
