Hi everyone, I am having trouble cutting frozen sections from visceral fat. I have never cut only fat before. I am also cutting liver sections and they are perfect, but when I try to cut the fat it is not working. The temperature of the cryostat is ( -20) degree Celsius. I wonder if anyone has ever done this. I was thinking that if I lower the temperature or fix the fat with formol-calcium I may have success.
Iliana Dimitrova, MSc, RT Histology Supervisor Room 2808 Medical Education and Laboratory Support Services (MELSS) Faculty of Medicine Memorial University St. John's, NL This electronic communication is governed by the terms and conditions at http://www.mun.ca/cc/policies/electronic_communications_disclaimer_2011.php _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet