Carmen, If the tissues have been fixed in formalin, then this would not be a problem, but if not, why not freeze the tissues in OCT (or similar) and store it at -70oC before cryotomy, rather than placing it in a sucrose solution?
Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Carmen Maria Garcia Pascual Sent: Tuesday, 11 October 2011 1:10 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] ovarian cryosections Hello everybody: I am here requesting your help another time ;) I want to make ovarian cryosections and I want to know if there is any problem if I leave the ovaries in 30% sucrose at 4ºC overnigth. Thank you very much. Kind regards, Carmen _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************************* _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
