On 10/10/2011 1:37 PM, sarah Tabatabaei wrote:
Hi histoneters,
I have a question regarding Sodium Cacodylate.
In our lab, after fixing our human tissue, we usually leave it in 10% sucrose
in double distilled water for a couple of days to remove all the fixatives out
of it. Recently, I used a solution of 10% sucrose in 0.1 ml Sodium Cacodylate,
instead of the plain distilled water thing. I have a feeling I have ruined them
for IHC. right? Can someone tell me what I have done to my tissue? I am
supposed to do immunohistochemistry and some histology on these tissues.
Looking forward to hear from you
Sarah
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Why do you think the specimens are ruined?
Certainly no problem with the histology, cacodylate buffer has been used
for many years for EM. Its use has declined as less-toxic alternatives
have been found.
As for immuno, try it, what have you got to lose?
And why did you use cacodylate in the first place?
Geoff
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Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
675 Hoes Lane, Piscataway, NJ 08854
voice: (732)-235-4583
mcaul...@umdnj.edu
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