Hello I would like to ask how many time do you put the mice head in xylene after series of Ethanol wash in the way of making paraffin block. I also did all the steps as well, but for embryonic day 17-18, even after observing clearing in xylene and moving to the several times in Gurr/paraplast I find the head to be soft and the initial part of head from which we start to cut is white and when I removed the flecks of wax and touched it was still soft. I am not getting what to do. Pathik
On Tue, Oct 11, 2011 at 11:01 PM, Kraniofacial Biology < kraniofacialbiol...@gmail.com> wrote: > Hello Histonetters > I am new to this site. I am a clinician and very fresh to research. I was > doing whole mount cartilage and bone staining of whole head of mice and was > surprised to see whole mouse head being used for experiment. I was wondering > if there is any other staining method which can be used in place of the > whole mount cartilage and bone staining that would stain the sections on a > slide and works on cartilage and bone. This would also save the whole mouse > to be used and many slides can also be used for other experiments. > Regards > Pathik > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
