CD3 - rabbit polyclonal Ab from ThermoScientific (cat. # RB-360) works on mouse FFPE tissue.

Jan Shivers
Senior Scientist
Histology/IHC/EM Section Head
Pathology Teaching Program
University of Minnesota
Veterinary Diagnostic Laboratory
1333 Gortner Ave.
St. Paul, MN  55108
612-624-7297
shive...@umn.edu

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----- Original Message ----- From: "Yu, Jian" <y...@upmc.edu>
To: <histonet@lists.utsouthwestern.edu>
Sent: Wednesday, October 26, 2011 2:44 PM
Subject: [Histonet] Abs for detecting immune cells in mouse FFPE sections


Dear all,

It will be greatly appreciated if you can share experience/information on the Abs against immune cells (vendor and cat#) that work for IHC or IF using mouse paraffin sections. Info on alternative Abs for these populations can be helpful too. I have a small research lab with limited budget for buying new Abs.

Gr-1 (granulocytes)
B220 (B cells)
CD3 (T cells)

Thank you!

Jian Yu, Ph.D.
University of Pittsburgh

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