CD3 Rabbit monoclonal from Fisher (MA1-39552) works fine in IF on mouse paraffin sections, B220 rat monoclonal from eBioscience (14-0452-85) works fine in IF on mouse paraffin sections. Used both in 1:100 dilution.
Anatoli Gleiberman, PhD Director of Histopathology Cleveland Biolabs, Inc 73 High Street Buffalo, NY 14203 phone:716-849-6810 ext.354 fax:716-849-6817 e-mail: agleiber...@cbiolabs.com -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Yu, Jian Sent: Wednesday, October 26, 2011 3:44 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Abs for detecting immune cells in mouse FFPE sections Dear all, It will be greatly appreciated if you can share experience/information on the Abs against immune cells (vendor and cat#) that work for IHC or IF using mouse paraffin sections. Info on alternative Abs for these populations can be helpful too. I have a small research lab with limited budget for buying new Abs. Gr-1 (granulocytes) B220 (B cells) CD3 (T cells) Thank you! Jian Yu, Ph.D. University of Pittsburgh _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This communication may contain privileged information. It is intended solely for the use of the addressee. If you are not the intended recipient, you are strictly prohibited from disclosing, copying, distributing or using any of this information. If you received this communication in error, please contact the sender immediately and destroy the material in its entirety, whether electronic or hard copy. This communication may contain nonpublic information about individuals and businesses subject to the restrictions of the Gramm-Leach-Bliley Act. You may not directly or indirectly reuse or redisclose such information for any purpose other than to provide the services for which you are receiving the information. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
