Hello, Do 30 minutes at 37 degrees on a glass slide. take from oven and apply gentle pressure to center of block and slide complex, cool to RT and repeat 1 or two times.
Helen -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Patricia F Lott Sent: Friday, October 28, 2011 1:13 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] need help with TMA We have recently started doing tissue microarrays. The problem we have is that some of the small cores drop out after the first few sections. I think my problem is in the step where we are supposed to melt the donor and recipient blocks together. We tried 5 minutes at 37 degrees, and then minutes at 60 degrees x3, with cooling to room temp in between. Any suggestions? Thanks, Patty Lott, UAB _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
