Good morning! I work with ovarie and mice uterus embebbed in oct. I get 8um frozen sections and put them un super frost slides, the I leave the slides in the fridge until use them. When I am going to perform the immunohistochemistry I leave the slides 1 minute at rt to atemperate the tissue, then I place the slides 10 minutes in acetone (stored at - 20ºC), whit this you fix the tissue and permeabilize it. with this protocol I never loss any sections. If you have any more question just write me. good luck, Carmen
******************************************************* Carmen María García Pascual FIVI/INCLIVA/Facultad de Medicina Universidad de Valencia Departamento de P.O.G (Laboratorios) 96.386.40.48 Avd. Blasco Ibañez 17 46010, Valencia ******************************************************* _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet