Realy recovered? No! René J. --- On Tue, 11/15/11, Patsy Ruegg <pru...@ihctech.net> wrote:
From: Patsy Ruegg <pru...@ihctech.net> Subject: [Histonet] is there a way to recover ruined tissues To: "'histo net'" <histonet@lists.utsouthwestern.edu> Date: Tuesday, November 15, 2011, 3:26 PM My tissue processor was loaded and although the technician thought they started it, the next morning the tissues were found dry in the retort with the processor not started. Another technician just started the machine so the tissues got processed as they would have been had it started the day before. The tissues look terrible, they shrank so much that the pieces started coming out of the cassettes even though they were plenty large enough in the first place. The tissues were found to be very small and hard but were embedded, sectioned and stained anyway (I was not informed about this until I started questioning why the tissues looked so bad). Of course the investigator is very upset that their tissues are ruined. Does anyone know if there is any way to try and recovery these tissues? They were mouse lungs and livers. The livers are not as bad as the lungs, the lungs were inflated and they look compressed and unrecognizable as lung tissue. If the investigator will ever speak to me again I am going to ask for the blocks back and try melting them down and perhaps try to rehydrate them somehow for reprocessing. Regards, Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech 12635 Montview Blvd. Ste.215 Aurora, CO 80045 720-859-4060 fax 720-859-4110 www.ihctech.net www.ihcrg.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet