Several sources for mouse antiRat CD31 (PECAM) were found by doing a simple Google search with keywords mouse antiRat CD31 immunohistochemistry. You can buy this monoclonal from Serotec, BD Biosciences and probably eBiosciences plus other companies that specialize in rodent antibodies. As for protocol, IHC World had a mouse antiRat CD31 procedure that worked on FFPE tissue with recommended retrieval - certainly worth a try with your reagents. Be sure to check application e.g. IHC on FFPE or frozen sections for any particular company's technical data sheet before buying the antibody. They sometimes only test on frozen sections or with the formalin free Zinc Tris Buffer (Becksteads ZSF fixative) fixed tissue for paraffin sections ala BD Biosciences Pharmingen.
As for working concentration, one should always do a dilution panel since your laboratory conditions and reagents will never be the same as in someone else's laboratory. We start our dilution panel at 10 ug/ml for solvent fixed fresh tissue frozen sections, and 20ug/ml for FFPE, and often fill in a wide gap if doing a serial dilution. The gap could be 1:500 then 1:1000, so we toss in a 1:750, and sometimes a 1:1500. Gayle M. Callis HTL/HT/MT(ASCP) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet