We use the Biocare concentrated antibody on the Biocare Intellipath. We are diluting at 1:100 and have very good consistent staining. Give me a call if you want to discuss further.
Karen Lahti, HT, QIHC Arizona Digestive Health 602-687-7468 On Jan 12, 2012, at 9:42 AM, "Freeman, Carol" <carol.free...@utoledo.edu> wrote: > > > I am curious if anyone is having trouble validating PMS2 antibody for > the MSI panel. We have worked this antbody up extensively and we are > finding that it is on either side of the spectrum just too light (it is > either light staining if any at all). We have stained a few cases > beautifully but nothing is consistent. We are looking to try another > vendor's antibodies, but were hoping for some feedback as to which labs > have found success in this area. I have never had trouble like this > with an antibody. The severe problem here is that with MSI testing, we > are looking for lack of staining with this antibody to show a positive > result so light patchy staining leaves a lot of room for error. Any > responses are appreciated. > > Thank you, > Carol E. Freeman HTL (ASCP) B.S. > Department of Pathology > University of Toledo Medical Center > 3000 Arlington Avenue > Toledo, OH 43614-5807 > carol.free...@utoledo.edu > (419)383-5639 > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet