Hi, I agree with Liz Chlipala's suggestion for the Serotec antibody. It works nicely. I recommend a protein block though. It does tend toward the background. I use Proteinase K rather than HIER.
Amos On Wed, Jan 25, 2012 at 11:22 AM, <histonet-requ...@lists.utsouthwestern.edu > wrote: > Message: 20 > Date: Wed, 25 Jan 2012 08:11:14 -0800 (PST) > From: Kim Merriam <kmerriam2...@yahoo.com> > Subject: [Histonet] neutrophil marker for FFPE mouse tissue > To: Histonet <histonet@lists.utsouthwestern.edu> > Message-ID: > <1327507874.8565.yahoomail...@web130104.mail.mud.yahoo.com> > Content-Type: text/plain; charset=us-ascii > > Hello! > > What antibodies are people using to stain for neutrophils in FFPE mouse > tissue? > > Kim > > > > Kim Merriam, MA, HT(ASCP)QIHC Cambridge, MA > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet