Referring to Her2 FISH, we perform this assay also on tissue with longer fixation. We found the protocol with "retrieval" in citratebuffer followed by protease digestion reliable. Also with SISH techniques I prefer long fixation more than underfixation.
Is there a chemical explanation, why the fluorochrome will fade after long fixation? Gudrun -----Ursprüngliche Nachricht----- Von: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] Im Auftrag von Kuhnla, Melissa Gesendet: Freitag, 03. Februar 2012 18:32 An: Gale Limron; histonet@lists.utsouthwestern.edu Betreff: RE: [Histonet] Breast IHC testing Hi, Keep in mind some of the responses I have seem so far are for just IHC. I think there is some literature stating that over fixation affects FISH less, but we perform FISH only and still stay under 48hrs. We use Pathvysion probes and the package insert also recommends not exceeding 48 hours, as signals will fade. We currently have a short step on the processor where tissue is held in 70% alcohol. We have certain specimen collection cut off times so everything is fixed between 6 and 4 hours. Three day, holiday weekends we have technologists come in on Saturday and embed everything. Melissa :) -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Gale Limron Sent: Friday, February 03, 2012 10:24 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Breast IHC testing I would like to know what other hospitals are doing with breast specimens that are resected on Friday and are in formalin longer than the maximum number of hours that CAP allows for ER/PR and HER2/neu testing. We are running into this problem since we don't currently work Saturday hours. Thank you! Gale Limron CT,HT (ASCP) Histology Supervisor Union Hospital 659 Boulevard Dover, Ohio 44622 330-343-3311 ext 2562 This e-mail is intended only for the person or entity to which it is addressed and may contain information that is privileged, confidential or otherwise protected from disclosure. Dissemination, distribution or copying of this e-mail or the information herein by anyone other than the intended recipient, or an employee or agent responsible for delivering the message to the intended recipient, is prohibited. If you received this message in error, please delete without copying and kindly e-mail a reply to inform us of the mistake in delivery. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information in this e-mail, and any attachments therein, is confidential and for use by the intended addressee only. If this message is received by you in error please do not disseminate or read further. Please reply to the sender that you have received the message in error, then delete the message. Although Catholic Health Services of Long Island attempts to sweep e-mail and attachments for viruses, it does not guarantee that either are virus-free and accepts no liability for any damage sustained as a result of viruses. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet