We do rotate our alcohols after eosin to minimize this problem. We recycle our alcohol from there so there is a cost savings.
Every lab has their setup that works best for them while pleasing the pathologist(s) too. I love this forum because you can get informed opinions! On Tue, Feb 7, 2012 at 4:32 PM, Victor A. Tobias <vtob...@uw.edu> wrote: > In a previous job we used Pro-Par and recycled it too for many years. I > don't know if I would label it a drawback, but I felt the dehydrating steps > after eosin required changing more often then if using xylene. There were > days when we would get the dreaded bleeding eosin. Keep your reagents fresh > and you shouldn't have any problems. We were hand coverslipping back then. > > Victor > > Victor Tobias HT(ASCP) > Clinical Applications Analyst > Harborview Medical Center > Dept of Pathology Room NJB244 > Seattle, WA 98104 > vtob...@u.washington.edu > 206-744-2735 > 206-744-8240 Fax > ================================================= > Privileged, confidential or patient identifiable information may be > contained in this message. This information is meant only for the use of > the intended recipients. If you are not the intended recipient, or if the > message has been addressed to you in error, do not read, disclose, > reproduce, distribute, disseminate or otherwise use this > transmission. Instead, please notify the sender by reply e-mail, and then > destroy all copies of the message and any attachments. > > -----Original Message----- > From: histonet-boun...@lists.utsouthwestern.edu [mailto: > histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Thomas Jasper > Sent: Tuesday, February 07, 2012 10:30 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Pro-Par > > Hi Folks, > > > > Just curious...anyone using Pro-Par care to share an opinion? Have > considered it off and on over the years and now wondering what folks > think. Also, anyone using it with tape coverslippers? Maybe this has > been asked and I wasn't keeping up with the thread. > > > > Thanks, > > Tom Jasper > > > > Thomas Jasper HT (ASCP) BAS > > Histology Supervisor > > Central Oregon Regional Pathology Services > > Bend, Oregon 97701 > > 541/693-2677 > > tjas...@copc.net > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Jen Campbell, HT(ASCP) Supervisor of Technical Services Muhlbauer Dermatopathology Laboratory 61 Monroe Avenue, Ste B Pittsford NY 14534 P: 585.586.5166 F: 585.586.3137 IMPORTANT NOTICE: This e-mail and any attachments may contain confidential or sensitive information which is, or may be, legally privileged or otherwise protected by law from further disclosure. It is intended only for the addressee. If you received this in error or from someone who was not authorized to send it to you, please do not distribute, copy or use it or any attachments. Please notify the sender immediately by reply e-mail and delete this from your system. Thank you for your cooperation. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet