Re:  SOP for release of tissues and consent form

Rhonda,
We do not have an SOP but if you have a fax, I can send you a form that we use.

Garret Miyamoto
Tripler Army Medical Center

----- Original Message -----
From: histonet-requ...@lists.utsouthwestern.edu
Date: Wednesday, March 7, 2012 8:04 am
Subject: Histonet Digest, Vol 100, Issue 9
To: histonet@lists.utsouthwestern.edu


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> Today's Topics:
> 
>   1. RE: xylene substitute for processing (Marcum, Pamela A)
>   2. unsubscribe (Sparks, Joanne)
>   3. Re: RE: xylene substitute for processing
>      (Grantham, Andrea L - (algranth))
>   4. Re: RE: xylene substitute for processing (Jennifer MacDonald)
>   5. modified Davidson's fixative on testes (Louro, Pedro)
>   6. Histotech Job Opening in TN-Permanent/Direct Hire (Melissa 
> Phelan)   7. SOP for release of tissues & consent form (Rhonda Ford)
>   8. mesocolon lymphnode treatment with aceton (Gudrun Lang)
> 
> 
> -------------------------------------------------------------------
> ---
> 
> Message: 1
> Date: Wed, 7 Mar 2012 15:58:13 +0000
> From: "Marcum, Pamela A" <
> Subject: [Histonet] RE: xylene substitute for processing
> To: "'Masood, Sameena Kathryn'" <,
>       "Cross, Kelly" <, "Histonet Listserv (E-mail)
>       (histonet@lists.utsouthwestern.edu)"
>       <
> Message-ID:
>       <
> Content-Type: text/plain; charset="us-ascii"
> 
> Some people have issues with the smell and the other option is Anatech ProPar 
> or Richard Allan Thermo non-aliphatic hydrocarbon subs which have very low 
> odor and are also about the same price.  We can't use the orange smell in our 
> lab as some people have allergies and others just don't like it.  Both work 
> types work very well and it is a lab choice for the options, not a quality 
> choice.  Be aware most processor companies still require xylene in the clean 
> cycle to keep your system healthy.
> 
> Pam Marcum
> 
> -----Original Message-----
> From: histonet-boun...@lists.utsouthwestern.edu 
> [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Masood, 
> Sameena Kathryn
> Sent: Wednesday, March 07, 2012 9:36 AM
> To: Cross, Kelly; Histonet Listserv (E-mail) 
> (histonet@lists.utsouthwestern.edu)
> Subject: [Histonet] RE: xylene substitute for processing
> 
> Kelly,
> 
> Histo-Clear can be directly substituted for Xylene at every point in 
> processing and staining.  It's completely non-toxic (even has a food grade 
> rating), is made from oranges, and smells delicious. You don't have to work 
> in a hood and it seems to make tissue less brittle than Xylene also. Some 
> guys in our Histology Core told us about it and we have been happy with it. 
> You can purchase it from the manufacturer, National Diagnostics, or Fisher.
> 
> http://www.nationaldiagnostics.com/product_info.php?products_id=175
> 
> Good luck,
> 
> Kathryn
> 
> 
> 
> 
> 
> -----Original Message-----
> From: histonet-boun...@lists.utsouthwestern.edu 
> [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Cross, Kelly
> Sent: Monday, March 05, 2012 10:24 AM
> To: Histonet Listserv (E-mail) (histonet@lists.utsouthwestern.edu)
> Subject: [Histonet] xylene substitute for processing
> 
> Greetings Histonet!
> 
> Does anyone use xylene substitutes for routine over-night processing? If so, 
> what do you use and does it have any adverse effect your special stains?
> 
> Thank you in advance for your help,
> Kelly
> 
> Kelly S. Cross B.S., HT (ASCP)
> Medical Laboratory Supervisor
> Veterinary Pathobiology
> Texas Veterinary Medical Center
> Texas A&M University
> College Station, TX 77843-4467
> 979-862-3658 Office
> 979-845-5149 Lab
> 
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> 
> 
> 
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> 
> ------------------------------
> 
> Message: 2
> Date: Wed, 7 Mar 2012 11:09:05 -0500
> From: "Sparks, Joanne" <
> Subject: [Histonet] unsubscribe
> To: <
> Message-ID:
>       <
> Content-Type: text/plain;     charset="us-ascii"
> 
>                                     Please unsubscribe from histonet.
> 
> 
> 
> ------------------------------
> 
> Message: 3
> Date: Wed, 7 Mar 2012 08:12:24 -0800
> From: "Grantham, Andrea L - (algranth)" <
> Subject: Re: [Histonet] RE: xylene substitute for processing
> Cc: HISTONET <
> Message-ID: <
> Content-Type: text/plain; charset="us-ascii"
> 
> I have been using Clear Rite 3 for years on animal tissue in a VIP for 
> overnite processing and it is great. I get it from Fisher. There has never 
> been a problem with special stains but I use xylene on my stain lines.
> 
> Andi Grantham
> 
> 
> 
> 
> On Mar 7, 2012, at 8:35 AM, Masood, Sameena Kathryn wrote:
> 
> > Kelly,
> > 
> > Histo-Clear can be directly substituted for Xylene at every point in 
> > processing and staining.  It's completely non-toxic (even has a food grade 
> > rating), is made from oranges, and smells delicious. You don't have to work 
> > in a hood and it seems to make tissue less brittle than Xylene also. Some 
> > guys in our Histology Core told us about it and we have been happy with it. 
> > You can purchase it from the manufacturer, National Diagnostics, or Fisher.
> > 
> > http://www.nationaldiagnostics.com/product_info.php?products_id=175
> > 
> > Good luck,
> > 
> > Kathryn
> > 
> > 
> > 
> > 
> > 
> > -----Original Message-----
> > From: histonet-boun...@lists.utsouthwestern.edu 
> > [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Cross, Kelly
> > Sent: Monday, March 05, 2012 10:24 AM
> > To: Histonet Listserv (E-mail) (histonet@lists.utsouthwestern.edu)
> > Subject: [Histonet] xylene substitute for processing
> > 
> > Greetings Histonet!
> > 
> > Does anyone use xylene substitutes for routine over-night processing? If 
> > so, what do you use and does it have any adverse effect your special stains?
> > 
> > Thank you in advance for your help,
> > Kelly
> > 
> > Kelly S. Cross B.S., HT (ASCP)
> > Medical Laboratory Supervisor
> > Veterinary Pathobiology
> > Texas Veterinary Medical Center
> > Texas A&M University
> > College Station, TX 77843-4467
> > 979-862-3658 Office
> > 979-845-5149 Lab
> > 
> > _______________________________________________
> > Histonet mailing list
> > Histonet@lists.utsouthwestern.edu
> > http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> > 
> > 
> > 
> > _______________________________________________
> > Histonet mailing list
> > Histonet@lists.utsouthwestern.edu
> > http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> > 
> 
> 
> ------------------------------
> 
> Message: 4
> Date: Wed, 7 Mar 2012 08:14:43 -0800
> From: Jennifer MacDonald <
> Subject: Re: [Histonet] RE: xylene substitute for processing
> To: "Marcum, Pamela A" <
> Cc: "Histonet Listserv \(E-mail\)
>       \(histonet@lists.utsouthwestern.edu\)"
>       <, "'Masood,    Sameena Kathryn'"
>       <,
>       histonet-boun...@lists.utsouthwestern.edu, "Cross,      Kelly"
>       <
> Message-ID:
>       <
> Content-Type: text/plain; charset="US-ASCII"
> 
> One thing to take into consideration that many of the xylene substitutes 
> take longer to clear than xylene.  You may have to adjust your processing 
> schedule.  We use a xylene substitute with success.
> 
> 
> 
> 
> "Marcum, Pamela A" < 
> Sent by: histonet-boun...@lists.utsouthwestern.edu
> 03/07/2012 07:58 AM
> 
> To
> "'Masood, Sameena Kathryn'" <, "Cross, 
> Kelly" <, "Histonet Listserv (E-mail) 
> (histonet@lists.utsouthwestern.edu)" <
> cc
> 
> Subject
> [Histonet] RE: xylene substitute for processing
> 
> 
> 
> 
> 
> 
> Some people have issues with the smell and the other option is Anatech 
> ProPar or Richard Allan Thermo non-aliphatic hydrocarbon subs which have 
> very low odor and are also about the same price.  We can't use the orange 
> smell in our lab as some people have allergies and others just don't like 
> it.  Both work types work very well and it is a lab choice for the 
> options, not a quality choice.  Be aware most processor companies still 
> require xylene in the clean cycle to keep your system healthy.
> 
> Pam Marcum
> 
> -----Original Message-----
> From: histonet-boun...@lists.utsouthwestern.edu 
> [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Masood, 
> Sameena Kathryn
> Sent: Wednesday, March 07, 2012 9:36 AM
> To: Cross, Kelly; Histonet Listserv (E-mail) 
> (histonet@lists.utsouthwestern.edu)
> Subject: [Histonet] RE: xylene substitute for processing
> 
> Kelly,
> 
> Histo-Clear can be directly substituted for Xylene at every point in 
> processing and staining.  It's completely non-toxic (even has a food grade 
> rating), is made from oranges, and smells delicious. You don't have to 
> work in a hood and it seems to make tissue less brittle than Xylene also. 
> Some guys in our Histology Core told us about it and we have been happy 
> with it. You can purchase it from the manufacturer, National Diagnostics, 
> or Fisher.
> 
> http://www.nationaldiagnostics.com/product_info.php?products_id=175
> 
> Good luck,
> 
> Kathryn
> 
> 
> 
> 
> 
> -----Original Message-----
> From: histonet-boun...@lists.utsouthwestern.edu 
> [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Cross, 
> Kelly
> Sent: Monday, March 05, 2012 10:24 AM
> To: Histonet Listserv (E-mail) (histonet@lists.utsouthwestern.edu)
> Subject: [Histonet] xylene substitute for processing
> 
> Greetings Histonet!
> 
> Does anyone use xylene substitutes for routine over-night processing? If 
> so, what do you use and does it have any adverse effect your special 
> stains?
> 
> Thank you in advance for your help,
> Kelly
> 
> Kelly S. Cross B.S., HT (ASCP)
> Medical Laboratory Supervisor
> Veterinary Pathobiology
> Texas Veterinary Medical Center
> Texas A&M University
> College Station, TX 77843-4467
> 979-862-3658 Office
> 979-845-5149 Lab
> 
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> 
> 
> 
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> Confidentiality Notice: This e-mail message, including any attachments,
> is for the sole use of the intended recipient(s) and may contain
> confidential and privileged information.  Any unauthorized review,
> use, disclosure or distribution is prohibited.  If you are not the 
> intended recipient, please contact the sender by reply
> e-mail and destroy all copies of the original message..
> 
> 
> _______________________________________________
> Histonet mailing list
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> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> 
> 
> 
> ------------------------------
> 
> Message: 5
> Date: Wed, 7 Mar 2012 11:55:26 -0500
> From: "Louro, Pedro" <
> Subject: [Histonet] modified Davidson's fixative on testes
> To: <
> Message-ID: <
> Content-Type: text/plain; charset="us-ascii"
> 
> Hello to all Histo techs and Happy early Histotechnology Professionals
> Day.
> 
> 
> 
> I wanted to know if anyone out there is using modified Davidson's
> fixative to fix rat testes and staining with PAS.
> 
> We have a pathologists that is looking for certain cells within the
> testes and thought that this might work.
> 
> 
> 
> Thanks in advance for any information.
> 
> 
> 
> Pedro Louro
> 
> Technologist
> 
> Histology/Immunohistochemistry 
> 
> 
> 
> 
> 
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> ------------------------------
> 
> Message: 6
> Date: Wed, 07 Mar 2012 12:21:50 -0500
> From: Melissa Phelan <
> Subject: [Histonet] Histotech Job Opening in TN-Permanent/Direct Hire
> To: <
> Message-ID: <
> Content-Type: text/plain;     charset="US-ASCII"
> 
> Hello All,
> 
> I have a permanent/full time job opening for a qualified Histotech in the
> Knoxville, TN area. Please contact me for details if you are interested.
> Everyone have a great day!!!
> -- 
> Melissa Phelan, President Laboratory Staffing
> Allied Search Partners
> http://www.linkedin.com/in/melissaphelan
> <http://www.linkedin.com/in/melissaphelan> P: 888-388-7571
> F: 888-388-7572
> C: 407-697-1175
> www.alliedsearchpartners.com
> 
> 
> 
> 
> 
> 
> ------------------------------
> 
> Message: 7
> Date: Wed, 7 Mar 2012 12:23:48 -0500
> From: Rhonda Ford <
> Subject: [Histonet] SOP for release of tissues & consent form
> To: histonet@lists.utsouthwestern.edu
> Message-ID:
>       <
> Content-Type: text/plain; charset=ISO-8859-1
> 
> Does anyone have a procedure for the release of tissues along with a
> consent form that they like would like to share?  Thanks in advance.
> 
> -- 
> Rhonda Ford, Histology Lab
> Henry County Hospital
> (765) 521-1148
> 
> 
> ------------------------------
> 
> Message: 8
> Date: Wed, 7 Mar 2012 18:24:57 +0100
> From: "Gudrun Lang" <
> Subject: [Histonet] mesocolon lymphnode treatment with aceton
> To: <
> Message-ID: <
> Content-Type: text/plain;     charset="us-ascii"
> 
> Dear all!
> 
> Does anyone out there use this method for mesocolon clearing with aceton? I
> heard only a few facts about it and would like to hear about personal
> experience.
> 
> The mesocolon is first fixed in NBF, then put in pure aceton, then treated
> with a teasing roller and put in a "pipe" with holes. The tissue is
> compressed to squeeze out the fat to form a kind of sausage.
> 
> The sausage is sliced up, the slices are put into cassettes and processed.
> 
> I don't know the exact details. If you have the access to this article, you
> can read more (I don't have). 
> 
> http://journals.lww.com/ajsp/Abstract/2012/02000/Optimal_Lymph_Node_Harvest_
> in_Rectal_Cancer__UICC.5.aspx
> 
> or this:
> 
> http://www.springerlink.com/content/748617k331l06143/
> 
> 
> 
> thank you
> 
> 
> 
> Gudrun Lang
> 
> 
> 
> Biomed. Analytikerin
> 
> Histolab, Linz, Austria
> 
> 
> 
> ------------------------------
> 
> _______________________________________________
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> 
> End of Histonet Digest, Vol 100, Issue 9
> ****************************************

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