I agree with Rene. A control (undigested) slide is necessary.

Geoff

On 3/15/2012 1:47 AM, Jennifer MacDonald wrote:
Hi All,
At a local lab when a pathologist orders a PAS diastase the
histotechnicians do just one slide with diastase.  They do not do an
undigested slide.  How would the pathologist know if the digested slide
had a glycogen to begin with?  Am I over thinking this?
Thanks,
Jennifer
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Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
675 Hoes Lane, Piscataway, NJ 08854
voice: (732)-235-4583
mcaul...@umdnj.edu
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