Good Morning and Happy Friday fellow histonetters :) I am looking into getting a Tissue Micro Array Device to make some nice multi-tissue (normal) and multi-tumor blocks for controls, validations and lot checks for IHC. Has anyone any advice for purchasing one, any favorites or any we should stay from....Any responses are appreciated.
Thanks in advance ;) Carol Freeman -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of histonet-requ...@lists.utsouthwestern.edu Sent: Friday, March 23, 2012 8:46 AM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 100, Issue 32 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-requ...@lists.utsouthwestern.edu You can reach the person managing the list at histonet-ow...@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. RE: Nocardia sp in FFPE (gayle callis) 2. Subject: [Histonet] Paraffin Block Shipping Question (White, Lisa M.) 3. RE: re: April fools prank (Podawiltz, Thomas) 4. Fungus control (Cynthia Robinson) 5. softening cartilage (Gerjets, Diane M [V PTH]) 6. Re: Fungus control ( thernandez...@yahoo.com ) 7. Retention Standards (histot...@imagesbyhopper.com) 8. RE: re: April fools prank (O'Donnell, Bill) 9. RE: Retention Standards (Bernice Frederick) 10. RE: softening cartilage (Elizabeth Chlipala) 11. RE: Retention Standards (histot...@imagesbyhopper.com) 12. RE: Retention Standards (histot...@imagesbyhopper.com) 13. Re: Retention Standards (Rich Yeo) 14. RE: Retention Standards (histot...@imagesbyhopper.com) 15. Re: Fixatives for breast (Richard Cartun) 16. Re: Nocardia procedure (Tony Reilly) 17. Part Time Histotech with Benefits near Portland, OR (Katelin Lester) 18. Post question (marc centofante) 19. Problems using tomato lectin (James S.) 20. Mouse abs on mouse tissue method (James S.) 21. PROTOCOL FOR COLOR CODING BIOPSY CASSETTES (Bernadette del Rosario) ---------------------------------------------------------------------- Message: 1 Date: Thu, 22 Mar 2012 11:34:14 -0600 From: "gayle callis" <gayle.cal...@bresnan.net> Subject: [Histonet] RE: Nocardia sp in FFPE To: "Histonet" <histonet@lists.utsouthwestern.edu> Message-ID: <001101cd0852$01a196c0$04e4c440$@bresnan.net> Content-Type: text/plain; charset="us-ascii" Jeanine, I always recall what the third edition AFIP manual (green) had to say about staining Nocardia successfully. Unfortunately, I didn't see an antibody for Nocardia. Several stains work and maybe have to do a combination of stains correlating results using adjacent sections? First choice would be GMS but you have to do some extra times in silver solution according to AFIP manual/Luna. In order to get complete development of filaments if different types/strains are suspected, run two slides with one at 60 minutes and the other at 90 minutes in the silver staining solution. Be sure to use chromic acid as the oxidizer. Churukian Shenk had a modified GMS that is supposed to work too, but nothing was said about extended times in methenamine silver solution. Fite's Rojas-Darby-Nochomovitz Method for Norcardia, TB and Leprae. This is similar to Fites but uses a Ziehl Neelsen carbol fuchsin modified with mineral oil (from Luna's Histopathological Methods and Color Atlas book) Luna Parker Method for Nocardia Asteroides (Luna book) with filaments appearing red. Brown and Brenn should show the organism too. Good luck Gayle M. Callis HTL/HT/MT(ASCP) You Wrote: I need the absolute best, most fool-proof special stain protocol for the staining of Nocardia in FFPE tissue. Thanks in advance! Jeanine H. Bartlett, BS HT(ASCP), QIHC Centers for Disease Control and Prevention Infectious Diseases Pathology Branch 1600 Clifton Road, NE MS/G-32 Atlanta, Ga 30333 404-639-3590 Jeanine.bartlett <@t> cdc.hhs.gov ------------------------------ Message: 2 Date: Thu, 22 Mar 2012 14:15:36 -0400 From: "White, Lisa M." <lisa.whi...@va.gov> Subject: Subject: [Histonet] Paraffin Block Shipping Question To: <histonet@lists.utsouthwestern.edu> Message-ID: <2b2ecf33934f5d4996d8be03efdf397609767...@vhav09msga3.v09.med.va.gov> Content-Type: text/plain; charset="us-ascii" EXAKT Technologies: Cold D-Pak for transport stock# MD8702V06; Frozen d-Pak for transport stock # MD8703V06 With current regulations on labeling and shipping of diagnostic material if you need to ship on ice or dry ice the above company has pre-labeled containers with all the federal requirements, etc as well as all the required packaging for the specimen and documents. We have to take training every year and it was explained to us that if something leaks all the powers that be out there go after the person who has their name on the shipping label (also whoever packs it has to be the one to do the label with their name). Blocks with a cold pack is a good idea during warm days. After such a mild winter I bet summer will be interesting. Also recently I was trying to find something to put a block in to mail and I had been putting them in a side loaded 5 slide container but could only get one in each one (but they cost $$$). I had been coverslipping and looked down at the empty coverslip container, VIOLA! Take the packaging out and you can get 2-3 blocks in there and they won't get squashed in the shipment and the container is free. Lisa White, HT(ASCP) Supervisory HT James H. Quillen VAMC PO Box 4000 Corner of Veterans Way and Lamont PLMS 113 Mountain Home, TN 37684 423-979-3567 423-979-3401 fax ------------------------------ Message: 3 Date: Thu, 22 Mar 2012 14:20:26 -0400 From: "Podawiltz, Thomas" <tpodawi...@lrgh.org> Subject: RE: [Histonet] re: April fools prank To: Kara Lee <karabo...@hotmail.com>, "histonet@lists.utsouthwestern.edu" <histonet@lists.utsouthwestern.edu> Message-ID: <38667e7fb77ecd4e91bfaeb8d986386324fb7e2...@lrghexvs1.practice.lrgh.org> Content-Type: text/plain; charset="us-ascii" I used to be real bad about pulling pranks in my younger days. If fact, there were three of use in our Navy lab that pranked each other all the time. Then to the horror of the rest of the staff we joined forces and starting pranking everyone else. It finally got to the point that all we had to do was make a comment about something and watch everyone's paranoia go up. Funny thing was on April first we never did a thing...and that drove the staff nuts. Tom Podawiltz HT (ASCP) Histology Section Head/Laboratory Safety Officer. LRGHealthcare Laconia, NH 03246 603-524-3211 ext: 3220 -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Kara Lee Sent: Thursday, March 22, 2012 11:59 AM To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] re: april fools prank One must always consider who the prank is being pulled on. If the person is sensitive or not quite in line with your own "Humor" a prank should not be pulled. As people working in the science industry, we have all been trained to observe. If a person is not the type to deal well with that type of humor, don't bother them and let them be. Or, ask if they want to be included in playing a prank on someone else who lives for seeing what their co-workers are doing to them next..I had a co-worker who was bummed when we were all too busy to mess with each others stuff one April 1st one year. Pranks and jokes should always make the other person laugh or smile just as much as you do, and don't forget to help them clean up the mess you made...but I think we all know that, right? ;) Enjoy life with your co-worker friends, and laugh! I mean, we do spend over 50% of our lives with these people. Cheers everyone! Kara > From: s...@stowers.org > To: hmarlat...@gmail.com; histonet@lists.utsouthwestern.edu > Date: Thu, 22 Mar 2012 07:14:41 -0500 > Subject: RE: [Histonet] re: april fools prank > CC: > > Heather, I see no harm in a fake spider. That would be fun. But, I totally > agree with what Barry just wrote. Some of these other pranks are out of > line, in my opinion. I'm all for having fun and enjoying the spirit of the > day, but I don't believe in aggravating or embarrassing someone for your > satisfaction. I have also pulled the hot dog trick on a resident > pathologist. He loved it. > Sharon > > > > -----Original Message----- > From: histonet-boun...@lists.utsouthwestern.edu > [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of heather > marlatt > Sent: Wednesday, March 21, 2012 10:25 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] re: april fools prank > > I've been known to leave a fake spider in an embedder for the morning > person.....although it wasn't april fools just for fun it got a great > reaction :) _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet THIS MESSAGE IS CONFIDENTIAL. This e-mail message and any attachments are proprietary and confidential information intended only for the use of the recipient(s) named above. If you are not the intended recipient, you may not print,distribute, or copy this message or any attachments. If you have received this communication in error, please notify the sender by return e-mail and delete this message and any attachments from your computer. Any views or opinions expressed are solely those of the author and do not necessarily represent those of LRGHealthcare. ------------------------------ Message: 4 Date: Thu, 22 Mar 2012 14:24:09 -0400 From: "Cynthia Robinson" <robin...@mercyhealth.com> Subject: [Histonet] Fungus control To: "histonet" <histonet@lists.utsouthwestern.edu> Message-ID: <4f6b27f9020000af00008...@nodcdmg2.no.trinity-health.org> Content-Type: text/plain; charset=US-ASCII All, We have a lung case that is loaded with fungus. Is anyone in need? Cindi Robinson HT(ASCP) Mercy Medical Center Dunes Medical Laboratories 350 W Anchor Dr Dakota Dunes SD 57049 phone-712-279-2768 robin...@mercyhealth.com ------------------------------ Message: 5 Date: Thu, 22 Mar 2012 18:31:50 +0000 From: "Gerjets, Diane M [V PTH]" <dmnel...@iastate.edu> Subject: [Histonet] softening cartilage To: "histonet@lists.utsouthwestern.edu" <histonet@lists.utsouthwestern.edu> Message-ID: <56a357dcb599a04eafc9c954b0a33dc20159d...@itsdag2d.its.iastate.edu> Content-Type: text/plain; charset="us-ascii" I'm sorry if this topic has already been discussed, I don't get on the histonet very often. Our lab will be getting a research project of horse cartilage. We don't have a good protocol for softening cartilage. We would appreciate any suggestions that any one might have. Thank you so much! Diane ------------------------------ Message: 6 Date: Thu, 22 Mar 2012 13:32:52 -0500 From: " thernandez...@yahoo.com " <thernandez...@yahoo.com> Subject: Re: [Histonet] Fungus control To: robin...@mercyhealth.com,"histonet" <histonet@lists.utsouthwestern.edu> Message-ID: <860854.13965...@smtp110.mail.bf1.yahoo.com> Content-Type: text/plain; charset=utf-8 Yes,we could definitely use some fungus. How can we arrange to get it ? Sent from my HTC smartphone on the Now Network from Sprint! ----- Reply message ----- From: "Cynthia Robinson" <robin...@mercyhealth.com> To: "histonet" <histonet@lists.utsouthwestern.edu> Subject: [Histonet] Fungus control Date: Thu, Mar 22, 2012 1:24 pm All, We have a lung case that is loaded with fungus. Is anyone in need? Cindi Robinson HT(ASCP) Mercy Medical Center Dunes Medical Laboratories 350 W Anchor Dr Dakota Dunes SD 57049 phone-712-279-2768 robin...@mercyhealth.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 7 Date: Thu, 22 Mar 2012 14:37:38 -0400 From: <histot...@imagesbyhopper.com> Subject: [Histonet] Retention Standards To: <histonet@lists.utsouthwestern.edu> Message-ID: <008601cd085a$deabc3d0$9c034b70$@imagesbyhopper.com> Content-Type: text/plain; charset=US-ASCII Does anyone have specific web links for CLIA, CAP, JC and, more specifically for me, Florida specimen retention guidelines? I want to update my retention policy, but I want to ensure that I am using the strictest of the guidelines. I am looking for guidelines on retention of service worksheets, specimen worksheets, requisitions etc and all things paper as well. THANKS for any help and direction you can provide, Michelle ------------------------------ Message: 8 Date: Thu, 22 Mar 2012 12:40:19 -0600 From: "O'Donnell, Bill" <billodonn...@catholichealth.net> Subject: RE: [Histonet] re: April fools prank To: "Podawiltz, Thomas" <tpodawi...@lrgh.org>, "Kara Lee" <karabo...@hotmail.com>, <histonet@lists.utsouthwestern.edu> Message-ID: <4940df6d1c5fdf48931b6966aaef93954d5...@chimsx08.chi.catholichealth.net> Content-Type: text/plain; charset="ISO-8859-1" Tom, Not always a simple, fun prank, but bordering on the, I don't know......psycopathic? (I won't mention them, as someone might actually do them, then I'd have all those court appearences to deal with and phycological evaluations.... I just don't have that kind of time anymore) Of all the stunts we pulled, the ONLY one that ever got me in trouble was putting carbonated water in someones waterbath. Certainly one of the more benign efforts. I will likely do a lot of time in purgatory for some of those, mostly because they still cause me to smile, even after all these years. And to think we have become respected professionals and pillars of society. Who'd-a-thunk it? - Bill -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Podawiltz, Thomas Sent: Thursday, March 22, 2012 1:20 PM To: Kara Lee; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] re: April fools prank I used to be real bad about pulling pranks in my younger days. If fact, there were three of use in our Navy lab that pranked each other all the time. Then to the horror of the rest of the staff we joined forces and starting pranking everyone else. It finally got to the point that all we had to do was make a comment about something and watch everyone's paranoia go up. Funny thing was on April first we never did a thing...and that drove the staff nuts. Tom Podawiltz HT (ASCP) Histology Section Head/Laboratory Safety Officer. LRGHealthcare Laconia, NH 03246 603-524-3211 ext: 3220 -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Kara Lee Sent: Thursday, March 22, 2012 11:59 AM To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] re: april fools prank One must always consider who the prank is being pulled on. If the person is sensitive or not quite in line with your own "Humor" a prank should not be pulled. As people working in the science industry, we have all been trained to observe. If a person is not the type to deal well with that type of humor, don't bother them and let them be. Or, ask if they want to be included in playing a prank on someone else who lives for seeing what their co-workers are doing to them next..I had a co-worker who was bummed when we were all too busy to mess with each others stuff one April 1st one year. Pranks and jokes should always make the other person laugh or smile just as much as you do, and don't forget to help them clean up the mess you made...but I think we all know that, right? ;) Enjoy life with your co-worker friends, and laugh! I mean, we do spend over 50% of our lives with these people. Cheers everyone! Kara > From: s...@stowers.org > To: hmarlat...@gmail.com; histonet@lists.utsouthwestern.edu > Date: Thu, 22 Mar 2012 07:14:41 -0500 > Subject: RE: [Histonet] re: april fools prank > CC: > > Heather, I see no harm in a fake spider. That would be fun. But, I totally agree with what Barry just wrote. Some of these other pranks are out of line, in my opinion. I'm all for having fun and enjoying the spirit of the day, but I don't believe in aggravating or embarrassing someone for your satisfaction. I have also pulled the hot dog trick on a resident pathologist. He loved it. > Sharon > > > > -----Original Message----- > From: histonet-boun...@lists.utsouthwestern.edu > [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of > heather marlatt > Sent: Wednesday, March 21, 2012 10:25 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] re: april fools prank > > I've been known to leave a fake spider in an embedder for the morning > person.....although it wasn't april fools just for fun it got a great > reaction :) _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet THIS MESSAGE IS CONFIDENTIAL. This e-mail message and any attachments are proprietary and confidential information intended only for the use of the recipient(s) named above. If you are not the intended recipient, you may not print,distribute, or copy this message or any attachments. If you have received this communication in error, please notify the sender by return e-mail and delete this message and any attachments from your computer. Any views or opinions expressed are solely those of the author and do not necessarily represent those of LRGHealthcare. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This electronic mail and any attached documents are intended solely for the named addressee(s) and contain confidential information. If you are not an addressee, or responsible for delivering this email to an addressee, you have received this email in error and are notified that reading, copying, or disclosing this email is prohibited. If you received this email in error, immediately reply to the sender and delete the message completely from your computer system. ------------------------------ Message: 9 Date: Thu, 22 Mar 2012 18:43:57 +0000 From: Bernice Frederick <b-freder...@northwestern.edu> Subject: RE: [Histonet] Retention Standards To: "histot...@imagesbyhopper.com" <histot...@imagesbyhopper.com>, "histonet@lists.utsouthwestern.edu" <histonet@lists.utsouthwestern.edu> Message-ID: <62c639732d3f274daced033ebdf6adaf1e227...@chcspmbx4.ads.northwestern.edu> Content-Type: text/plain; charset="us-ascii" You can find the CAP retention policies on their web site http://www.cap.org/apps/cap.portal?_nfpb=true&cntvwrPtlt_actionOverride=%2Fportlets%2FcontentViewer%2Fshow&_windowLabel=cntvwrPtlt&cntvwrPtlt%7BactionForm.contentReference%7D=laboratory_accreditation%2Fnewsletters%2F0301_2newsletter.html&_state=maximized&_pageLabel=cntvwr Bernice Frederick HTL (ASCP) Senior Research Tech Pathology Core Facility ECOGPCO-RL Robert. H. Lurie Cancer Center Northwestern University 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 b-freder...@northwestern.edu -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of histot...@imagesbyhopper.com Sent: Thursday, March 22, 2012 1:38 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Retention Standards Does anyone have specific web links for CLIA, CAP, JC and, more specifically for me, Florida specimen retention guidelines? I want to update my retention policy, but I want to ensure that I am using the strictest of the guidelines. I am looking for guidelines on retention of service worksheets, specimen worksheets, requisitions etc and all things paper as well. THANKS for any help and direction you can provide, Michelle _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 10 Date: Thu, 22 Mar 2012 12:46:41 -0600 From: Elizabeth Chlipala <l...@premierlab.com> Subject: [Histonet] RE: softening cartilage To: "'Gerjets, Diane M [V PTH]'" <dmnel...@iastate.edu>, "histonet@lists.utsouthwestern.edu" <histonet@lists.utsouthwestern.edu> Message-ID: <14E2C6176416974295479C64A11CB9AE011390CC5A09@SBS2K8.premierlab.local> Content-Type: text/plain; charset="us-ascii" Diane You should not need to soften cartilage, we section cartilage from many species without any additional steps to routine processing. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308-1592 (303) 682-3949 office (303) 682-9060 fax (303) 881-0763 cell www.premierlab.com Ship to address: 1567 Skyway Drive, Unit E Longmont, CO 80504 -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Gerjets, Diane M [V PTH] Sent: Thursday, March 22, 2012 12:32 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] softening cartilage I'm sorry if this topic has already been discussed, I don't get on the histonet very often. Our lab will be getting a research project of horse cartilage. We don't have a good protocol for softening cartilage. We would appreciate any suggestions that any one might have. Thank you so much! Diane _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 11 Date: Thu, 22 Mar 2012 14:46:36 -0400 From: <histot...@imagesbyhopper.com> Subject: RE: [Histonet] Retention Standards To: "'Bernice Frederick'" <b-freder...@northwestern.edu>, <histonet@lists.utsouthwestern.edu> Message-ID: <008a01cd085c$1f945280$5ebcf780$@imagesbyhopper.com> Content-Type: text/plain; charset=US-ASCII Bernice, Thanks for that one! That's one down. :o) JC anyone? Florida?? :) Michelle -----Original Message----- From: Bernice Frederick [mailto:b-freder...@northwestern.edu] Sent: Thursday, March 22, 2012 2:44 PM To: histot...@imagesbyhopper.com; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Retention Standards You can find the CAP retention policies on their web site http://www.cap.org/apps/cap.portal?_nfpb=true&cntvwrPtlt_actionOverride=%2Fp ortlets%2FcontentViewer%2Fshow&_windowLabel=cntvwrPtlt&cntvwrPtlt%7BactionFo rm.contentReference%7D=laboratory_accreditation%2Fnewsletters%2F0301_2newsle tter.html&_state=maximized&_pageLabel=cntvwr Bernice Frederick HTL (ASCP) Senior Research Tech Pathology Core Facility ECOGPCO-RL Robert. H. Lurie Cancer Center Northwestern University 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 b-freder...@northwestern.edu -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of histot...@imagesbyhopper.com Sent: Thursday, March 22, 2012 1:38 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Retention Standards Does anyone have specific web links for CLIA, CAP, JC and, more specifically for me, Florida specimen retention guidelines? I want to update my retention policy, but I want to ensure that I am using the strictest of the guidelines. I am looking for guidelines on retention of service worksheets, specimen worksheets, requisitions etc and all things paper as well. THANKS for any help and direction you can provide, Michelle _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ----- No virus found in this message. Checked by AVG - www.avg.com Version: 2012.0.1913 / Virus Database: 2114/4886 - Release Date: 03/22/12 ------------------------------ Message: 12 Date: Thu, 22 Mar 2012 15:22:48 -0400 From: <histot...@imagesbyhopper.com> Subject: RE: [Histonet] Retention Standards To: "'Bernice Frederick'" <b-freder...@northwestern.edu>, <histonet@lists.utsouthwestern.edu> Message-ID: <009401cd0861$2e0f3190$8a2d94b0$@imagesbyhopper.com> Content-Type: text/plain; charset=US-ASCII Here is an updated CAP compendium retention guidelines list. This was last updated in March, 2010 http://www.cap.org/apps/cap.portal?_nfpb=true&cntvwrPtlt_actionOverride=%2Fp ortlets%2FcontentViewer%2Fshow&_windowLabel=cntvwrPtlt&cntvwrPtlt%7BactionFo rm.contentReference%7D=policies%2Fpolicy_appPP.html&_state=maximized&_pageLa bel=cntvwr Michelle -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of histot...@imagesbyhopper.com Sent: Thursday, March 22, 2012 2:47 PM To: 'Bernice Frederick'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Retention Standards Bernice, Thanks for that one! That's one down. :o) JC anyone? Florida?? :) Michelle -----Original Message----- From: Bernice Frederick [mailto:b-freder...@northwestern.edu] Sent: Thursday, March 22, 2012 2:44 PM To: histot...@imagesbyhopper.com; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Retention Standards You can find the CAP retention policies on their web site http://www.cap.org/apps/cap.portal?_nfpb=true&cntvwrPtlt_actionOverride=%2Fp ortlets%2FcontentViewer%2Fshow&_windowLabel=cntvwrPtlt&cntvwrPtlt%7BactionFo rm.contentReference%7D=laboratory_accreditation%2Fnewsletters%2F0301_2newsle tter.html&_state=maximized&_pageLabel=cntvwr Bernice Frederick HTL (ASCP) Senior Research Tech Pathology Core Facility ECOGPCO-RL Robert. H. Lurie Cancer Center Northwestern University 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 b-freder...@northwestern.edu -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of histot...@imagesbyhopper.com Sent: Thursday, March 22, 2012 1:38 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Retention Standards Does anyone have specific web links for CLIA, CAP, JC and, more specifically for me, Florida specimen retention guidelines? I want to update my retention policy, but I want to ensure that I am using the strictest of the guidelines. I am looking for guidelines on retention of service worksheets, specimen worksheets, requisitions etc and all things paper as well. THANKS for any help and direction you can provide, Michelle _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ----- No virus found in this message. Checked by AVG - www.avg.com Version: 2012.0.1913 / Virus Database: 2114/4886 - Release Date: 03/22/12 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ----- No virus found in this message. Checked by AVG - www.avg.com Version: 2012.0.1913 / Virus Database: 2114/4886 - Release Date: 03/22/12 ------------------------------ Message: 13 Date: Thu, 22 Mar 2012 12:35:49 -0700 (PDT) From: Rich Yeo <histocontr...@yahoo.com> Subject: Re: [Histonet] Retention Standards To: "histot...@imagesbyhopper.com" <histot...@imagesbyhopper.com>, 'Bernice Frederick' <b-freder...@northwestern.edu>, "histonet@lists.utsouthwestern.edu" <histonet@lists.utsouthwestern.edu> Message-ID: <1332444949.74857.yahoomail...@web140417.mail.bf1.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 There is another update from Jan. 2012. It should be on thier web site R ________________________________ From: "histot...@imagesbyhopper.com" <histot...@imagesbyhopper.com> To: 'Bernice Frederick' <b-freder...@northwestern.edu>; histonet@lists.utsouthwestern.edu Sent: Thursday, March 22, 2012 3:22 PM Subject: RE: [Histonet] Retention Standards Here is an updated CAP compendium retention guidelines list. This was last updated in March, 2010 http://www.cap.org/apps/cap.portal?_nfpb=true&cntvwrPtlt_actionOverride=%2Fp ortlets%2FcontentViewer%2Fshow&_windowLabel=cntvwrPtlt&cntvwrPtlt%7BactionFo rm.contentReference%7D=policies%2Fpolicy_appPP.html&_state=maximized&_pageLa bel=cntvwr Michelle -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of histot...@imagesbyhopper.com Sent: Thursday, March 22, 2012 2:47 PM To: 'Bernice Frederick'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Retention Standards Bernice, Thanks for that one! That's one down. :o) JC anyone? Florida?? :) Michelle -----Original Message----- From: Bernice Frederick [mailto:b-freder...@northwestern.edu] Sent: Thursday, March 22, 2012 2:44 PM To: histot...@imagesbyhopper.com; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Retention Standards You can find the CAP retention policies on their web site http://www.cap.org/apps/cap.portal?_nfpb=true&cntvwrPtlt_actionOverride=%2Fp ortlets%2FcontentViewer%2Fshow&_windowLabel=cntvwrPtlt&cntvwrPtlt%7BactionFo rm.contentReference%7D=laboratory_accreditation%2Fnewsletters%2F0301_2newsle tter.html&_state=maximized&_pageLabel=cntvwr Bernice Frederick HTL (ASCP) Senior Research Tech Pathology Core Facility ECOGPCO-RL Robert. H. Lurie Cancer Center Northwestern University 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 b-freder...@northwestern.edu -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of histot...@imagesbyhopper.com Sent: Thursday, March 22, 2012 1:38 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Retention Standards Does anyone have specific web links for CLIA, CAP, JC and, more specifically for me, Florida specimen retention guidelines? I want to update my retention policy, but I want to ensure that I am using the strictest of the guidelines. I am looking for guidelines on retention of service worksheets, specimen worksheets, requisitions etc and all things paper as well. THANKS for any help and direction you can provide, Michelle _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ----- No virus found in this message. Checked by AVG - www.avg.com Version: 2012.0.1913 / Virus Database: 2114/4886 - Release Date: 03/22/12 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ----- No virus found in this message. Checked by AVG - www.avg.com Version: 2012.0.1913 / Virus Database: 2114/4886 - Release Date: 03/22/12 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 14 Date: Thu, 22 Mar 2012 16:50:24 -0400 From: <histot...@imagesbyhopper.com> Subject: RE: [Histonet] Retention Standards To: "'Rich Yeo'" <histocontr...@yahoo.com>, "'Bernice Frederick'" <b-freder...@northwestern.edu>, <histonet@lists.utsouthwestern.edu> Message-ID: <000c01cd086d$6bac16b0$43044410$@imagesbyhopper.com> Content-Type: text/plain; charset="us-ascii" I just got off the phone with CAP and the 2010 revision is their most recent. :o) I also found the CLIA retention guidelines. they can be found at the following URL: https://www.cms.gov/CLIA/03_Interpretive_Guidelines_for_Laboratories.asp 2 down, 2 to go! Michelle From: Rich Yeo [mailto:histocontr...@yahoo.com] Sent: Thursday, March 22, 2012 3:36 PM To: histot...@imagesbyhopper.com; 'Bernice Frederick'; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Retention Standards There is another update from Jan. 2012. It should be on thier web site R _____ From: "histot...@imagesbyhopper.com" <histot...@imagesbyhopper.com> To: 'Bernice Frederick' <b-freder...@northwestern.edu>; histonet@lists.utsouthwestern.edu Sent: Thursday, March 22, 2012 3:22 PM Subject: RE: [Histonet] Retention Standards Here is an updated CAP compendium retention guidelines list. This was last updated in March, 2010 http://www.cap.org/apps/cap.portal?_nfpb=true <http://www.cap.org/apps/cap.portal?_nfpb=true&cntvwrPtlt_actionOverride=%2F p> &cntvwrPtlt_actionOverride=%2Fp ortlets%2FcontentViewer%2Fshow&_windowLabel=cntvwrPtlt&cntvwrPtlt%7BactionFo rm.contentReference%7D=policies%2Fpolicy_appPP.html&_state=maximized&_pageLa bel=cntvwr Michelle -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of histot...@imagesbyhopper.com Sent: Thursday, March 22, 2012 2:47 PM To: 'Bernice Frederick'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Retention Standards Bernice, Thanks for that one! That's one down. :o) JC anyone? Florida?? :) Michelle -----Original Message----- From: Bernice Frederick [mailto:b-freder...@northwestern.edu] Sent: Thursday, March 22, 2012 2:44 PM To: histot...@imagesbyhopper.com; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Retention Standards You can find the CAP retention policies on their web site http://www.cap.org/apps/cap.portal?_nfpb=true <http://www.cap.org/apps/cap.portal?_nfpb=true&cntvwrPtlt_actionOverride=%2F p> &cntvwrPtlt_actionOverride=%2Fp ortlets%2FcontentViewer%2Fshow&_windowLabel=cntvwrPtlt&cntvwrPtlt%7BactionFo rm.contentReference%7D=laboratory_accreditation%2Fnewsletters%2F0301_2newsle tter.html&_state=maximized&_pageLabel=cntvwr Bernice Frederick HTL (ASCP) Senior Research Tech Pathology Core Facility ECOGPCO-RL Robert. H. Lurie Cancer Center Northwestern University 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 b-freder...@northwestern.edu -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of histot...@imagesbyhopper.com Sent: Thursday, March 22, 2012 1:38 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Retention Standards Does anyone have specific web links for CLIA, CAP, JC and, more specifically for me, Florida specimen retention guidelines? I want to update my retention policy, but I want to ensure that I am using the strictest of the guidelines. I am looking for guidelines on retention of service worksheets, specimen worksheets, requisitions etc and all things paper as well. THANKS for any help and direction you can provide, Michelle _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 15 Date: Thu, 22 Mar 2012 17:09:52 -0400 From: "Richard Cartun" <rcar...@harthosp.org> Subject: Re: [Histonet] Fixatives for breast To: "LaSalette Charette" <las.chare...@comcast.net>, "histonet@lists.utsouthwestern.edu" <histonet@lists.utsouthwestern.edu> Message-ID: <4f6b5ce0.7400.007...@harthosp.org> Content-Type: text/plain; charset=US-ASCII 1.) Formalin, 2.) Formalin, and 3.) Formalin. Richard Richard W. Cartun, MS, PhD Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 Office (860) 545-2204 Fax >>> LaSalette Charette <las.chare...@comcast.net> 3/21/2012 12:59 PM >>> What are the best fixatives for breast specimens? Also what the most commonly used fixatives out there? LaSalette _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 16 Date: Fri, 23 Mar 2012 09:23:54 +1000 From: "Tony Reilly" <tony_rei...@health.qld.gov.au> Subject: Re: [Histonet] Nocardia procedure To: "Jeanine (CDC/OID/NCEZID) Bartlett" <j...@cdc.gov>, "histonet@lists.utsouthwestern.edu" <histonet@lists.utsouthwestern.edu> Message-ID: <4f6c4129.411c.003...@health.qld.gov.au> Content-Type: text/plain; charset="us-ascii" Hi Jeanine I have always just used a Fite and it has worked well. If you use a Gram you will find that they are Gram variable in their staining. regards Tony Tony Reilly B.App.Sc. , M.Sc. Chief Scientist, Anatomical Pathology Pathology Queensland-PA Laboratory _________________________________________________ Clinical and Statewide Services Division| QueenslandHealth Level 1, Building 15,Princess Alexandra Hospital Ipswich Road,WOOLLOONGABBA Qld4102 Ph: 07 3176 2412 Mob: 0402 139411 Fax: 07 3176 2930 Email: tony_rei...@health.qld.gov.au Web: www.health.qld.gov.au/qhcss/ >>> "Bartlett, Jeanine (CDC/OID/NCEZID)" <j...@cdc.gov> 3/23/2012 1:54 am >>> Hi all, I need the absolute best, most fool-proof special stain protocol for the staining of Nocardia in FFPE tissue. Thanks in advance! Jeanine H. Bartlett, BS HT(ASCP), QIHC Centers for Disease Control and Prevention Infectious Diseases Pathology Branch 1600 Clifton Road, NE MS/G-32 Atlanta, Ga 30333 404-639-3590 jeanine.bartl...@cdc.hhs.gov _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ******************************************************************************** This email, including any attachments sent with it, is confidential and for the sole use of the intended recipient(s). This confidentiality is not waived or lost, if you receive it and you are not the intended recipient(s), or if it is transmitted/received in error. Any unauthorised use, alteration, disclosure, distribution or review of this email is strictly prohibited. The information contained in this email, including any attachment sent with it, may be subject to a statutory duty of confidentiality if it relates to health service matters. If you are not the intended recipient(s), or if you have received this email in error, you are asked to immediately notify the sender by telephone collect on Australia +61 1800 198 175 or by return email. You should also delete this email, and any copies, from your computer system network and destroy any hard copies produced. If not an intended recipient of this email, you must not copy, distribute or take any action(s) that relies on it; any form of disclosure, modification, distribution and/or publication of this email is also prohibited. Although Queensland Health takes all reasonable steps to ensure this email does not contain malicious software, Queensland Health does not accept responsibility for the consequences if any person's computer inadvertently suffers any disruption to services, loss of information, harm or is infected with a virus, other malicious computer programme or code that may occur as a consequence of receiving this email. Unless stated otherwise, this email represents only the views of the sender and not the views of the Queensland Government. ********************************************************************************** ------------------------------ Message: 17 Date: Thu, 22 Mar 2012 16:42:49 -0700 From: Katelin Lester <katelin09...@gmail.com> Subject: [Histonet] Part Time Histotech with Benefits near Portland, OR To: Histonet <histonet@lists.utsouthwestern.edu> Message-ID: <caoezybsmr6uxu2s0wpt8ggn5p+qnrbb9sczzqmzwdruwdnk...@mail.gmail.com> Content-Type: text/plain; charset=ISO-8859-1 Part time certified HT or HTL wanted for busy GI practice in Tualatin, Oregon. Candidate must be ASCP certified and meet CLIA requirements to perform gross dissection. GI experience preferred. This is a M-F position with no weekends , holidays or call. Includes Full Medical & Dental benefits plus a 401K program. Interested applicants should forward their resume to gba...@gispecialists.org ------------------------------ Message: 18 Date: Fri, 23 Mar 2012 07:54:07 -0400 From: marc centofante <marccentofa...@gmail.com> Subject: [Histonet] Post question To: histonet@lists.utsouthwestern.edu Message-ID: <calbveuaz3g+epdnejkbhp7qfo1dey3b-vuebw0mnd4kwonr...@mail.gmail.com> Content-Type: text/plain; charset=ISO-8859-1 I am not sure how to post to histonet but if I email this address and then it gets posted here is what I would like to post: My employer is looking to buy an anatomic pathology lab with frozen section service. Does anyone know of any labs for sale? -- Thank you, Marc Centofante marccentofa...@gmail.com ------------------------------ Message: 19 Date: Fri, 23 Mar 2012 12:25:27 +0000 From: "James S." <sonya.mar...@soton.ac.uk> Subject: [Histonet] Problems using tomato lectin To: "'histonet@lists.utsouthwestern.edu'" <histonet@lists.utsouthwestern.edu> Message-ID: <5be0438a6d85e645a67a4a2f58f41693f...@uos-msg00041-si.soton.ac.uk> Content-Type: text/plain; charset="us-ascii" Hi all, I've just purchased Texas-Red conjugated tomato lectin from Vector and have been trying it out on some frozen mouse liver sections but cant see anything. Protocol - fresh frozen sections 8um cut and air dried overnight. Fixed (10min) acetone, washed PBS, tomato lectin applied (I've done serial dilutions from 1/100 - 1/5000 in PBS/0.05% Tween) 1hr at room temp, wash PBS, mount Vectashield + dapi. Any suggestions? Thanks Sonya ------------------------------ Message: 20 Date: Fri, 23 Mar 2012 12:34:01 +0000 From: "James S." <sonya.mar...@soton.ac.uk> Subject: [Histonet] Mouse abs on mouse tissue method To: "'histonet@lists.utsouthwestern.edu'" <histonet@lists.utsouthwestern.edu> Message-ID: <5be0438a6d85e645a67a4a2f58f41693f...@uos-msg00041-si.soton.ac.uk> Content-Type: text/plain; charset="us-ascii" I've been trying out the method from IHC world to use mouse primary plus anti-mouse secondary abs on mouse tissue. Protocol; Fresh frozen sections (mouse spleen) cut 8um and air dried Fixed acetone 10min Wash PBS Block with 2.5% normal goat serum in PBS/Tw, 30min Block with unconjugated Fab fragment goat anti-mouse IgG (Jackson Immuno, diluted 1/10 in PBS/Tw), 2hrs room temp Wash PBS/Tw Seconary ab - DyLight488 conjugated Fab fragment goat anti-mouse IgG (Jackson Immuno, 1/500 PBS/Tw), 20min room temp Wash PBS/Tw Mount Blocking with unconjugated Fab did decrease background if I used an IgG goat anti-mouse secondary but the background was actually increased when I used the Fab goat anti-mouse!! Any suggestions? Thanks Sonya ------------------------------ Message: 21 Date: Fri, 23 Mar 2012 05:42:33 -0700 (PDT) From: Bernadette del Rosario <badzros...@yahoo.com> Subject: [Histonet] PROTOCOL FOR COLOR CODING BIOPSY CASSETTES To: "histonet@lists.utsouthwestern.edu" <histonet@lists.utsouthwestern.edu> Message-ID: <1332506553.66638.yahoomail...@web130204.mail.mud.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Good day histonetters.We are a new university hospital and setting up histopatholology lab.I used to work with white biopsy cassettes only but not technicolors.Got this boss who ask me protocols on colored cassettes etc...No idea about this.Is there any standard pattern which i can just base and copy (example skin-yellow;breast-pink etc..)Im trying to surf in the net but cant find..Please someone help me??? ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 100, Issue 32 ***************************************** _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet