Maxim, I have used and would recommend the Hacker H/I-76 Microtome Knife Sharpener. With this sharpener, honing and stropping a microtome knife takes only a few minutes, and results in a better knife edge than the automatic glass plate honing/stropping method. Also, as long as the knife edge has not been nicked or damaged, the microtome knife need only be stropped as needed. I like you choice to stay away from the disposable knife blades. I preferred using the solid knife to the disposable blades because of the strength and stability at the cutting edge (less of a chance for the chatter artifact). The very sharp, slightly biconcave edge on the blade (see profile B on their website, below) allows 4 micron sections to be easily sectioned from the paraffin block. A new H/I-76 can be ordered directly from Hacker, http://www.hackerinstruments.com/tissueprep.htm. For less of an investment... be wary, there are a few available on the secondary market. Keith Mikoff, HTL/HT(ASCP) Sacramento, California mik...@gmail.com
Message: 5 Date: Wed, 4 Apr 2012 23:20:15 +0300 From: Maxim Peshkov <maxim...@mail.ru> Subject: [Histonet] Automatic knife sharpener To: histonet@lists.utsouthwestern.edu Message-ID: <1182039392.20120404232...@mail.ru> Content-Type: text/plain; charset=windows-1251 Dear Histonetters! Can anybody to advise, what type automatic knife sharpener we can buy in Europe (Germany) instead Leica SP9000, which now is unavailable for sale? What vendors? We do not like a Shandon "Autosharp 5" type sharpener. Maxim Peshkov Russia, Taganrog. mailto:maxim...@mail.ru _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet