My experience with this is that the processing schedule is too long and probably too much heat. I had some skin that was grown in a well plate from human cells processed on a biopsy run, they cut fantastic then the gel rolled onto itself during staining. Once we reduced the processing schedule and took heat away on the processing reagents it was much better. And, try to use super sticky slides, such as those you would use with bone samples.
Carrie L. Schray Univ. of Michigan Medical School Unit for Laboratory Animal Medicine Ann Arbor, Michigan On 4/5/12, Amy Porter <port...@msu.edu> wrote: > To all: I have a client that we are attempting to stain H & E / as well as > immuno on FFPE mouse skin with matrigel plugs. These things section like a > dream and then when we attempt to stain only the matrigel part of the sample > is falling off. Does anyone have any tricks with this stuff they would be > willing to share? We are using charged slides and cutting at 5-6 microns > per the client. Thanks - > > > > Amy S. Porter, HT(ASCP) QIHC > > Michigan State University > > Investigative HistoPathology Laboratory > > William S. Spielman, Ph.D. - Director > > Patricia K. Senagore, M.D. - Consulting Pathologist > > Department of Physiology / Human Pathology > > Biomedical Physical Sciences Building > > 567 Wilson Road - Room 2133 > > East Lansing, MI 48824-3320 > > Phone: 517-884-5026 > > Fax: 517-432-1368 > > port...@msu.edu > > www.humanpathology.msu.edu > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet