Brains in particular need to be fixed real well If it's a whole brain what I've done is hang the brain by a mesh or strings into a large brain bucket so it's not touching the sides or bottom. Fix for few days then get you sections. I'd go textbook on the 3 mm thick sections for processing and don't over process that will cause them to be friable. Hate that. Try a few blocks a couple different ways and what kind of alcohol are you using? Reagent grade is fine. For processing well fixed brain I've had good success with a straight 30 min for every thing. Hope this helps Kim D
I'm out :) Sent from my iPhone On Apr 10, 2012, at 7:14 PM, Meryl Roberts <mery...@hotmail.com> wrote: > > > > Our lab processes a high number of autopsies; however we always seem to have > tissue that needs to be reprocessed; particularly brains. Does anyone out > there have any suggestions as to what an optimal processing cycle would be? > We are finding it hard to find a happy medium as there always seems to be > something that is underprocessed, or sometimes even overprocessed. Thanks. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
