Hi my boss requires me to counterstain some DAB immunohistochemistry with methyl green. I use for that purpose the methyl green in aquos solution 0.5% in 4.2 acetate buffer. The problem is that after the washes I dehydrate and the methyl green becomes extremely faint with the alcohols. My protocol requires long periods of alcoholic dehydration very graded. Is there any idea on how to save the methyl green. Can I use an alcoholic solution? Thank you very much.
Alonso _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet