I agree with Jackie, we use Ki-67 all of the time and I have never seen it stain apoptotic cells, could you possibly be dealing with some background staining due to the detection system used? I do not know what type of samples you are staining? We have 4 different Ki-67 antibodies we use depending upon the type of tissue we are staining. For human xenograft samples in a mouse background we use the rabbit polyclonal from Santa Cruz. We also use cleaved caspase 3 as a marker of apoptosis routinely.
Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308-1592 (303) 682-3949 office (303) 682-9060 fax (303) 881-0763 cell www.premierlab.com Ship to address: 1567 Skyway Drive, Unit E Longmont, CO 80504 -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jackie O'Connor Sent: Thursday, April 19, 2012 3:23 PM To: nmargar...@childrensmemorial.org; histonet-requ...@lists.utsouthwestern.edu Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] proliferation and apoptosis Ki67 does not stain apoptotic cells. Why do you have that impression? Caspase-3 is a great marker for apoptotic cells. TUNEL will show apoptosis as well as necrosis, but not proliferation. I've used Ki67 for proliferation and Caspase 3 for apoptosis routinely in cancer research for years. Jackie -----Original Message----- From: Margaryan, Naira <nmargar...@childrensmemorial.org> To: histonet-request <histonet-requ...@lists.utsouthwestern.edu> Cc: histonet <histonet@lists.utsouthwestern.edu> Sent: Thu, Apr 19, 2012 3:09 pm Subject: [Histonet] proliferation and apoptosis Hi histonetters, I am looking for the good markers to detect (separately) proliferation and apoptosis of cells in tumor sections. Unfortunately, KI-67 stains apoptotic bodies as well as proliferated cells; and the tunnel assay shows both apoptotic body and proliferation. Any suggestions for the FFPE tissue and the HRP protocol are appreciated. Thanks in advance, Naira _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
