From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Scott, Allison D Sent: Thursday, April 19, 2012 3:23 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] GMS on Toenail
Hello to all in histoland. We have a stubborn toenail that keeps coming off when we try to do a GMS stain on the ventana machine. Any suggestions on how to keep the section on the slide during the staining procedure. Allison Scott HT(ASCP) Histology Supervisor LBJ Hospital Houston, Texas Hello Allison, we do PAS and GMS on toenails just about every day since we have a nail clinic in our department. First we dip our slides in Stay On solution (SurgiPath) and allow them to dry then we soak the faced nail block in 4% Ammonium Hydroxide for 45-60 minutes before we cut a ribbon and place on slides. We let the slides sit in a 70 degree oven for about 45 minutes, we Deparaffinize, hydrate to DH2O and we stain on the Artisan Special Stain stainer. Works every time. If it keeps falling off you might need to do it manually. Good Luck -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of histonet-requ...@lists.utsouthwestern.edu Sent: Saturday, April 21, 2012 1:03 PM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 101, Issue 28 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-requ...@lists.utsouthwestern.edu You can reach the person managing the list at histonet-ow...@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Re: Histonet Digest, Vol 101, Issue 27 (Eric Tambutte) 2. Re: Pinning Specimens (Bob Richmond) 3. Immunofluorescence (Thotakura, Anil Kumar) 4. RE: GMS on Toenail (Britton, Josette C) 5. Leica Peloris Processor (Joe W. Walker, Jr.) 6. Re: Leica Peloris Processor (Richard Cartun) 7. Re: Leica Peloris Processor (Patrick Laurie) 8. Re: Histonet Digest, Vol 101, Issue 27 (Amos Brooks) ---------------------------------------------------------------------- Message: 1 Date: Fri, 20 Apr 2012 19:03:08 +0200 From: Eric Tambutte <etambu...@centrescientifique.mc> Subject: [Histonet] Re: Histonet Digest, Vol 101, Issue 27 To: <histonet@lists.utsouthwestern.edu> Message-ID: <1083480...@s15272523.onlinehome-server.info> Bonjour, Je suis absent du laboratoire jusqu'au jeudi 03 mai 2012. Je vous répondrai le plus rapidement possible. Eric Tambutté Thank you for your mail. I will be out of office till May 03rd 2012. I will respond to your e-mail as soon as possible. Thank you for your understanding. Best regards Eric Tambutté ------------------------------ Message: 2 Date: Fri, 20 Apr 2012 13:20:15 -0400 From: Bob Richmond <rsrichm...@gmail.com> Subject: [Histonet] Re: Pinning Specimens To: histonet@lists.utsouthwestern.edu Message-ID: <caoksrh5gcgsm6hesayw40jr0lfxssre_f42ze0hde1pve0e...@mail.gmail.com> Content-Type: text/plain; charset=ISO-8859-1 About pinning specimens so they fix flat: Margaret Horne notes the use of dental wax. I've used it to make small "boats" for the rather exacting procedure of pinning muscle biopsy specimens for electron microscopy. Here a 1 to 2 mm bundle of longitudinal fibers has to be gently stretched to its resting length, pinned in the "boat", and promptly fixed in glutaraldehyde. One technologist noted that she had a young pathologist who was "wet behind the ears" and wanted to pin specimens, and was looking to the older pathologists to bring him in line to The Way We've Always Done It. I try to remain "wet behind the ears", though after nearly 50 years of occasionally pinning specimens I find myself a little dry. We continue to lose skills in the gross room. Today I'm working with a service that has almost no ability to handle calcified tissue - I need to go to the hardware store and buy a hacksaw to replace the worn-out Satterlee saw, which I think was gathered up off a Civil War battlefield. Bob Richmond Samurai Pathologist Knoxville TN ------------------------------ Message: 3 Date: Fri, 20 Apr 2012 18:01:17 +0000 From: "Thotakura, Anil Kumar" <a.thotak...@imperial.ac.uk> Subject: [Histonet] Immunofluorescence To: "histonet@lists.utsouthwestern.edu" <histonet@lists.utsouthwestern.edu> Message-ID: <cbb762fa.14aa6%a.thotak...@imperial.ac.uk> Content-Type: text/plain; charset="us-ascii" Dear All, Can some one please send the protocol for immunofluorescence, I want to stain for FITC CD45.1 on mice spleens. Thank you very much for your help. Many Thanks, Anil kumar. ------------------------------ Message: 4 Date: Fri, 20 Apr 2012 14:54:29 -0400 From: "Britton, Josette C" <jcbrit...@cheshire-med.com> Subject: RE: [Histonet] GMS on Toenail To: "Scott, Allison D" <allison_sc...@hchd.tmc.edu>, <histonet@lists.utsouthwestern.edu> Message-ID: <f644cd64b2313f43bb3d2496b454cdac09a35...@cmc-ex01.cheshire-med.com> Content-Type: text/plain; charset="us-ascii" The way we got around that problem was to do a manual PAS stain instead! Josie Britton HT Cheshire Medical Center Keene NH -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Scott, Allison D Sent: Thursday, April 19, 2012 3:23 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] GMS on Toenail Hello to all in histoland. We have a stubborn toenail that keeps coming off when we try to do a GMS stain on the ventana machine. Any suggestions on how to keep the section on the slide during the staining procedure. Allison Scott HT(ASCP) Histology Supervisor LBJ Hospital Houston, Texas CONFIDENTIALITY NOTICE: If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail and any attachments from your computer system. To the extent the information in this e-mail and any attachments contain protected health information as defined by the Health Insurance Portability and Accountability Act of 1996 ("HIPAA"), PL 104-191; 45 CFR Parts 160 and 164; or Chapter 181, Texas Health and Safety Code, it is confidential and/or privileged. This e-mail may also be confidential and/or privileged under Texas law. The e-mail is for the use of only the individual or entity named above. If you are not the intended recipient, or any authorized representative of the intended recipient, you are hereby notified that any review, dissemination or copying of this e-mail and its attachments is strictly prohibited. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 5 Date: Fri, 20 Apr 2012 19:47:13 +0000 From: "Joe W. Walker, Jr." <joewal...@rrmc.org> Subject: [Histonet] Leica Peloris Processor To: "histonet@lists.utsouthwestern.edu" <histonet@lists.utsouthwestern.edu> Message-ID: <3c2378778400ad448ada6fd6bdb7cccc582...@rrmbx03.rrmc.local> Content-Type: text/plain; charset="us-ascii" Hello all, I am new to your listserve. I am wondering if anyone has experience with the Leica Peloris processor. Specifically, has anyone noticed if the higher processing temperatures has affected any IHC staining results or FISH results. Looking forward to responses, Joe W. Walker, Jr. SCT(ASCP)CM Anatomical Pathology Manager Rutland Regional Medical Center 160 Allen Street, Rutland, VT 05701 Phone: 802.747.1790 Fax:802.747.6525 NEW EMAIL: joewal...@rrmc.org<mailto:joewal...@rrmc.org> www.rrmc.org<http://www.rrmc.org> Our Vision: To be the Best Healthcare System in New England Rutland Regional...Vermont's 1st Hospital to Achieve Both ANCC Magnet Recognition(r) and the Governor's Award for Performance Excellence This message (and any included attachments) is from Rutland Regional Health Services and is intended only for the addressee(s). The information contained herein may include privileged or otherwise confidential information. Unauthorized review, forwarding, printing, copying, distributing, or using such information is strictly prohibited and may be unlawful. If you received this message in error, or have reason to believe you are not authorized to receive it, please promptly delete this message and notify the sender by e-mail. Thank You ------------------------------ Message: 6 Date: Fri, 20 Apr 2012 16:07:16 -0400 From: "Richard Cartun" <rcar...@harthosp.org> Subject: Re: [Histonet] Leica Peloris Processor To: "histonet@lists.utsouthwestern.edu" <histonet@lists.utsouthwestern.edu>, "Jr. Joe W. Walker" <joewal...@rrmc.org> Message-ID: <4f9189b4.7400.007...@harthosp.org> Content-Type: text/plain; charset=US-ASCII In my experience, no. Our current IHC/ISH is as good, if not better than what we were getting with our former processors. Richard Richard W. Cartun, MS, PhD Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 Office (860) 545-2204 Fax >>> "Joe W. Walker, Jr." <joewal...@rrmc.org> 4/20/2012 3:47 PM >>> Hello all, I am new to your listserve. I am wondering if anyone has experience with the Leica Peloris processor. Specifically, has anyone noticed if the higher processing temperatures has affected any IHC staining results or FISH results. Looking forward to responses, Joe W. Walker, Jr. SCT(ASCP)CM Anatomical Pathology Manager Rutland Regional Medical Center 160 Allen Street, Rutland, VT 05701 Phone: 802.747.1790 Fax:802.747.6525 NEW EMAIL: joewal...@rrmc.org<mailto:joewal...@rrmc.org> www.rrmc.org<http://www.rrmc.org> Our Vision: To be the Best Healthcare System in New England Rutland Regional...Vermont's 1st Hospital to Achieve Both ANCC Magnet Recognition(r) and the Governor's Award for Performance Excellence This message (and any included attachments) is from Rutland Regional Health Services and is intended only for the addressee(s). The information contained herein may include privileged or otherwise confidential information. Unauthorized review, forwarding, printing, copying, distributing, or using such information is strictly prohibited and may be unlawful. If you received this message in error, or have reason to believe you are not authorized to receive it, please promptly delete this message and notify the sender by e-mail. Thank You _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 7 Date: Fri, 20 Apr 2012 13:29:30 -0700 From: Patrick Laurie <foreig...@gmail.com> Subject: Re: [Histonet] Leica Peloris Processor To: "Joe W. Walker, Jr." <joewal...@rrmc.org> Cc: "histonet@lists.utsouthwestern.edu" <histonet@lists.utsouthwestern.edu> Message-ID: <CAKEyg-0babdOMrHwET04LxwkBwe9bj51vPO6oz7ehs=TWa5R=a...@mail.gmail.com> Content-Type: text/plain; charset=ISO-8859-1 We have 4 peloris processors, run many IHC and FISH stains, none of them have had any negative effects due to the increased temperatures. The increased temps aren't that high, 45 degrees for all the liquids (we use xylene) and 65 degrees for the paraffins. Good luck Patrick On Fri, Apr 20, 2012 at 12:47 PM, Joe W. Walker, Jr. <joewal...@rrmc.org>wrote: > Hello all, > > I am new to your listserve. I am wondering if anyone has experience with > the Leica Peloris processor. Specifically, has anyone noticed if the > higher processing temperatures has affected any IHC staining results or > FISH results. > > Looking forward to responses, > > Joe W. Walker, Jr. SCT(ASCP)CM > Anatomical Pathology Manager > Rutland Regional Medical Center > 160 Allen Street, Rutland, VT 05701 > Phone: 802.747.1790 Fax:802.747.6525 > NEW EMAIL: joewal...@rrmc.org<mailto:joewal...@rrmc.org> > www.rrmc.org<http://www.rrmc.org> > > Our Vision: To be the Best Healthcare System in New England > Rutland Regional...Vermont's 1st Hospital to Achieve Both ANCC Magnet > Recognition(r) and the Governor's Award for Performance Excellence > > This message (and any included attachments) is from Rutland Regional > Health Services and is intended only for the addressee(s). The information > contained herein may include privileged or otherwise confidential > information. Unauthorized review, forwarding, printing, copying, > distributing, or using such information is strictly prohibited and may be > unlawful. If you received this message in error, or have reason to believe > you are not authorized to receive it, please promptly delete this message > and notify the sender by e-mail. > > Thank You > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Patrick Laurie HT(ASCP)QIHC CellNetix Pathology & Laboratories 1124 Columbia Street, Suite 200 Seattle, WA 98104 plau...@cellnetix.com ------------------------------ Message: 8 Date: Fri, 20 Apr 2012 17:46:16 -0400 From: Amos Brooks <amosbro...@gmail.com> Subject: [Histonet] Re: Histonet Digest, Vol 101, Issue 27 To: histonet@lists.utsouthwestern.edu Message-ID: <CAC95ki908wAAmhw-Ny=wthj9mbak4qg6x1lkfikpcdfnsr3...@mail.gmail.com> Content-Type: text/plain; charset=ISO-8859-1 Hi, Strange issue with your Ki-67. I don't know what would do that unless it was non-specific background. Is it nuclear or cutoplasmic? If you are working in mice or other animals you could try labeling them with BRDu then detecting it with an anti BRDu antibody. Humans don't like being injected with BRDu then being sacrifices but lawyers do. Also Cleaved Caspase 3 works nicely and is probably more specific to apoptosis than Tunel which also picks up necrosis. Have a great weekend, Amos On Fri, Apr 20, 2012 at 1:00 PM, <histonet-requ...@lists.utsouthwestern.edu>wrote: > Message: 9 > Date: Thu, 19 Apr 2012 15:07:43 -0500 > From: "Margaryan, Naira" <nmargar...@childrensmemorial.org> > Subject: [Histonet] proliferation and apoptosis > To: "histonet-requ...@lists.utsouthwestern.edu" > <histonet-requ...@lists.utsouthwestern.edu> > Cc: "histonet@lists.utsouthwestern.edu" > <histonet@lists.utsouthwestern.edu> > Message-ID: > < > c1ba93040c6b9a4a8d84878f93fec36a0ab7d0e...@cmhexcc01mbx.childrensmemorial.org > > > > Content-Type: text/plain; charset="us-ascii" > > Hi histonetters, > > I am looking for the good markers to detect (separately) proliferation and > apoptosis of cells in tumor sections. Unfortunately, KI-67 stains apoptotic > bodies as well as proliferated cells; and the tunnel assay shows both > apoptotic body and proliferation. > > Any suggestions for the FFPE tissue and the HRP protocol are appreciated. > > Thanks in advance, > Naira > ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 101, Issue 28 ***************************************** The information contained in this e-mail message is intended only for the personal and confidential use of the recipient(s) named above. If the reader of this message is not the intended recipient or an agent responsible for delivering it to the intended recipient, you are hereby notified that you have received this document in error and that any review, dissemination, distribution, or copying of this message is strictly prohibited. If you have received this communication in error, please notify us immediately by e-mail, and delete the original message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet