Hello, I'm working with very small fish brains and would like to perform the modified Golgi-Cox stain of http://www.ncbi.nlm.nih.gov/pubmed/21228908 . As my area of interest is near the edge of the tissue I need to coat in egg yolk or gelatine to prevent the dark crust which forms on the tissue (is there another way?). Manipulating my tiny brains in this way in a raw unfixed state is likely to cause significant tissue damage. I have found one article where Golgi-Cox was used on neutral buffered formalin (NBF) fixed tissue (the entire pig brain, http://www.ncbi.nlm.nih.gov/pubmed/2459816) but I can't get hold of the article to actually see how this turned out. Has anyone had any experience using the Golgi-Cox stain on NBF tissue? Where there any problems? (I don't want to kill fish for nothing.)
On a related matter, anyone find any faults with the protocol/solutions at http://www.funjournal.org/images/stories/downloads/2011_Volume_10_Issue_1/wright_10_1_a85_a87.pdf ? Thanks -- Tyrone Genade http://tgenade.freeshell.org email: tgen...@gmail.com tel: +27-84-632-1925 (c) ******************************************************************************** Romans 6:23: The gift of God is eternal life through Christ Jesus our Lord. To find out how to receive this FREE gift visit http://www.alpha.org. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
