Thank you, Ian - the quote from Carson's book with be most helpful. I do not have access to reference texts in my current location - I very much appreciate your time to provide the quote. Good luck with your HTL!
-----Original Message----- From: Ian R Bernard <ibern...@uab.edu> To: Jackie O'Connor <b427...@aol.com>; histonet <histonet@lists.utsouthwestern.edu> Sent: Sun, May 13, 2012 4:48 pm Subject: RE: [Histonet] Can use some help with processing references Rather than research literature (lit), may I refer you to the foremost authority n histotechnology procedures: Histotechonology- A Self Instructional Text, 3rd dition by Frieda Carson and Christa Hladik, Chapter 2, page 38, paragraph 2: Tissue should remain in paraffin the shortest time necessary for good nfiltration because exposure to prolonged heat causes shrinkage and hardening." consider book the foremost (bible) reference on histotechnology. At least I ope so, since I'm studying for my HTL from this book. Note: There are lit at he end of this chapter that may go into more detail. The paragraph goes on to state that: " ...melted paraffin should be kept 2 to 4 egrees Celsius above the melting point because tissues exposed to overheated araffin during infiltration will over harden. Bottom-line, I would not leave tissues in paraffin over the weekend. If you are sing an automatic-closed system processor, your equipment should be able to ork on a delayed status, where the tissues will sit in 10 Neutral Buffered ormalin under vacuum (better for the tissue), all weekend, and start processing chedule on Sunday to come off on Monday morning. At least ours does. Hope this helps. Ian R. Bernard an R. Bernard, MSHA, HT (ASCP) 0th Medical Group- Anatomic Pathology Lab SAF Academy, CO 80840 -----Original Message----- rom: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] n Behalf Of Jackie O'Connor ent: Sunday, May 13, 2012 2:42 PM o: histonet@lists.utsouthwestern.edu ubject: [Histonet] Can use some help with processing references Hi Y'all - I'm kind of in a jam here - I'm looking for references on paraffin issue processing - can someone point me towards any literature that says eaving tissues in molten paraffin over the weekend is a bad idea? I need to rove a point, and need something to back me up. I'm out of the US doing a onsult, having trouble doing a lit search - I'm really not lazy. hanks in advance. ackie O' ______________________________________________ istonet mailing list isto...@lists.utsouthwestern.edu ttp://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet