Hi, I have done this. It works quite well. It would probably be best to use an alkaline phosphatase detection since the brilliant red of the fast red precipitate is more distinguishable from the brown DAB. (I am assuming you used HRP-DAB detection of the Tunel.) You needn't worry about the left over HRP since you'll be doing an antigen retrieval step which should wash them all out.
Amos On Wed, May 23, 2012 at 1:00 PM, <histonet-requ...@lists.utsouthwestern.edu>wrote: > Message: 16 > Date: Wed, 23 May 2012 08:03:17 -0700 > From: "Alicia R. Lange" <arla...@medicine.nevada.edu> > Subject: [Histonet] RESTAINING WITH KI67 > To: <histonet@lists.utsouthwestern.edu> > Message-ID: > <9809822f80b1414c90eb4438713c896801345...@medx.medicine.nevada.edu> > Content-Type: text/plain; charset="us-ascii" > > Hello Histoland! > > > > Has anyone restained tunel slides with ki67? Since tunel stains > apoptosis and ki67 proliferation, I was just going to restain with ki67. > I wanted to get some input before I did this. Thanks! > > > > Alicia Lange > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet